“…It is essential to employ appropriate metabolomics analysis to efficiently identify metabolites with low sensitivity. For example, untargeted metabolomics identified the presence of glutathione metabolites in healthy human plasma samples, which was associated with the antioxidant benefits of the broccoli diet [ 164 ]. However, glutathione and its precursors, glutamine, and cysteine, were transiently downregulated at 6 and 12 h after the broccoli diet [ 165 ], whereas fatty acid metabolites associated with lipid metabolism were consistently downregulated [ 165 ].…”