Plasma Metabolome Profiling by High-Performance Chemical Isotope-Labelling LC-MS after Acute and Medium-Term Intervention with Golden Berry Fruit (Physalis peruviana L.), Confirming Its Impact on Insulin-Associated Signaling Pathways

Abstract

Purpose: Golden berry (Physalis peruviana L.) is an exotic fruit exported from Colombia to different countries around the world. A review of the literature tends to demonstrate a hypoglycaemic effect with an improvement in insulin sensitivity after oral ingestion of fruit extracts in animal models. However, little is known about their potential effects in humans, and very little is known about the mechanisms involved. This study aimed at identifying discriminant metabolites after acute and chronic intake of golden berry. Method: An untargeted metabolomics strategy using high-performance chemical isotope-labelling LC-MS was applied. The blood samples of eighteen healthy adults were analysed at baseline, at 6 h after the intake of 250 g of golden berry (acute intervention), and after 19 days of daily consumption of 150 g (medium-term intervention). Results: Forty-nine and 36 discriminant metabolites were identified with high confidence, respectively, after the acute and medium-term interventions. Taking into account up- and downregulated metabolites, three biological networks mainly involving insulin, epidermal growth factor receptor (EGFR), and the phosphatidylinositol 3-kinase pathway (PI3K/Akt/mTOR) were identified. Conclusions: The biological intracellular networks identified are highly interconnected with the insulin signalling pathway, showing that berry intake may be associated with insulin signalling, which could reduce some risk factors related to metabolic syndrome. Primary registry of WHO.

Daily Consumption of Golden Berry (Physalis peruviana) Has Been Shown to Halt the Progression of Insulin Resistance and Obesity in Obese Rats with Metabolic Syndrome

“…ICA UCH-16-02) which are grown by the company Caribbean Exotic S.A.S. at the farm "La Bendición" (6.23779 latitude/75.32.45 longitude) in San Vicente Ferrer, Antioquia, Colombia [8,11].…”

“…This fruit is notably rich in antioxidants such as carotenoids, flavonoids, and phenolic acids, known for their role in preventing chronic diseases, including metabolic disorders. Furthermore, both in vitro and in vivo studies indicate that Physalis peruviana may possess anti-inflammatory, antidiabetic, and antihyperlipidemic properties [8].…”

Flash vacuum expansion, a low-cost and energy-efficient alternative process to produce high-quality fruit puree: Application to Physalis peruviana

“…There is great interest in the consumption and industrial use of this fruit for its nutritional and medicinal properties [ 2 , 3 ]. Recently, its functional value for health has been published, and insulin signaling has been shown to reduce the risk of type 2 diabetes [ 4 ].…”

Physicochemical characterization of cape gooseberry (Physalis peruviana L.) fruits ecotype Colombia during preharvest development and growth

“…In recent times, research on cape gooseberry ( Physalis Peruviana ) has increased considerably due to the greater relevance in the agro‐industrial sector that it has been gaining in several Latin American and African countries (Álvarez‐Herrera et al., 2021; Fischer & Melgarejo, 2020; Guevara‐Collazos et al., 2019; Vaillant et al., 2021). Cape gooseberry fruits have high agro‐industrial potential due to their high juice content (between 63.0% and 69.3% of fresh weight) and low seed content (4.45–5.04%).…”

“…In South Africa, annual yields of 8 t/ha can be expected in most soils and climates (Berriesforafrica, 2022). Cape gooseberry is a very attractive product for consumption due to its pleasant organoleptic characteristics (smell, taste, and appearance) and its nutritional properties (Fischer & Melgarejo, 2020; Olivares‐Tenorio et al., 2017; Vaillant et al., 2021). The main use of this product is fresh consumption due to its high nutritional value since it is a natural source of nutrients such as proteins (2.54–1.88 g/100 g), lipids (< 1.0%), sugars, dietary fibers such as pectin (1.85%), cellulose (4.29% to 6.64%), natural pigments such as carotenoids (3.62 and 22.36 µg/g) and essential macro and microelements, especially K, Mg, Cu, and Zn (Petkova, et al., 2021).…”