Plasma Metabolites and Gut Microbiota Are Associated With T cell Imbalance in BALB/c Model of Eosinophilic Asthma

Zhou, Yumei; Wang, Tieshan; Zhao, Xiaoshan; Wang, Ji; Wang, Qi

doi:10.3389/fphar.2022.819747

Cited by 9 publications

(10 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While, until now there are none research about the DL-glutamine, L-pyroglutamic acid, prostaglandin b1, prostaglandin e2, and 3,4-dihydroxyhydrocinnamic acid associated with genus_ Muriculum . 1,5-anhydro-d-sorbitol is associated with Treg/Th17 cell balance in allergic asthma mouse model ( Zhou et al, 2022 ). There are none research based on the penh value associated them.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Guominkang formula alleviate inflammation in eosinophilic asthma by regulating immune balance of Th1/2 and Treg/Th17 cells

Zhou

Zhang

et al. 2022

Front. Pharmacol.

Self Cite

View full text Add to dashboard Cite

The number of patients with allergic asthma is rising yearly, and hormonal drugs, such as dexamethasone, have unique advantages and certain limitations. In the treatment of allergic diseases especially allergic asthma, increasing the percentage or the function of immunosuppressive cells, such as Treg cells, may achieve a good effect. On the basis of good clinical results, we found that Guominkang (GMK) especially high-concentration GMK can achieve a similar effect with dexamethasone in controlling the symptoms of allergic asthma and inhibiting inflammation of allergic asthma. In our study, GMK can inhibit the recruitment of inflammatory cells, decrease mucus production, and reduce airway resistance. Besides, GMK can reconstruct the cellular immune balance of Th1/2 and Treg/Th17 cells. Metabolome results show that DL-glutamine, L-pyroglutamic acid, prostaglandin b1, prostaglandin e2, and 3,4-dihydroxyhydrocinnamic acid are the metabolic biomarkers and are associated with Th1/2 and Treg/Th17 cell balance. GMK can also change the gut microbiota in the allergic asthma mouse model. The genus_Muriculum, genus_(Clostridium) GCA900066575, genus_klebsiella, genus_Desulfovibrio, genus_Rikenellaceae RC9 gut group, family_Chitinophagaceae, family_Nocardioidaceae, and genus_Corynebacterium are gut microbiota biomarkers treated by GMK. Among these biomarkers, genus_Muriculum is the gut microbiota biomarker associated with Th1/2 and Treg/Th17 cell balance. Interestingly, we first found that DL-glutamine, L-pyroglutamic acid, prostaglandin b1, prostaglandin e2, and 3,4-dihydroxyhydrocinnamic acid are all associated with genus_Muriculum. GMK will be a new strategy for the treatment of eosinophilic asthma, and biomarkers will also be a new research direction.

show abstract

Section: Discussionmentioning

confidence: 99%

“…The amount of mRNA of the indicated genes after normalization of β -actin mRNA. The primers of GAPDH, Foxp3, RORγt, IFN-γ and IL-4 genes were as described as in a previous study ( Zhou et al, 2022 ).…”

Section: Methodsmentioning

confidence: 99%

Guominkang formula alleviate inflammation in eosinophilic asthma by regulating immune balance of Th1/2 and Treg/Th17 cells

Zhou

Zhang

et al. 2022

Front. Pharmacol.

Self Cite

View full text Add to dashboard Cite

show abstract

“… 23 Th2 cells mainly produce IL-4, IL-5 and IL-13, which increase the level of IgE in serum and recruit eosinophils. 24 Previous studies showed that the proportion and functional imbalance of Th1 and Th2 subsets form the main case mechanism of asthma attack, 25 as evidenced by a decline in the function of the Th1 cell subsets and the hyperfunction of theTh2 cell subsets. In line with this, the present study also revealed that the expression of Th2-related cytokines was increased in patients with CVA and CA.…”

Section: Discussionmentioning

confidence: 99%

Study on the Relationship Between Bronchoalveolar Lavage Fluid Cell Count, Th1/Th2 Cytokines and Pulmonary Function in Patients with Cough Variant Asthma

Chen¹,

Lin²,

Gu³

et al. 2022

JAA

View full text Add to dashboard Cite

This study aimed to compare lung function and airway inflammation among cough variant asthma (CVA), chronic cough and classical asthma (CA) and investigate the relationship between these indicators and their possible mechanisms of action in the progression of CVA to CA. Methods: 36 patients with chronic cough, 39 patients with CA, and 57 patients with CVA were included in this study. Pulmonary function tests, bronchial provocation tests and FeNO tests were performed. The patients' bronchoalveolar lavage fluid (BALF) was collected, the cells in BALF were counted, and the levels of Th1 and Th2 cytokines were detected. Results: The neutrophils, lymphocytes, and eosinophils in BALF in the CA and CVA groups were significantly higher than those in the chronic cough group. Also, they were negatively correlated with FEV1, FVC, and FEV1/FVC and positively correlated with IgE and FeNO. The expression of Th2-related cytokines was increased in CVA and CA patients, and it was positively correlated with FEV1, FVC and FEV1/FVC and negatively correlated with IgE and FeNO, while the results of Th1-related cytokines were the opposite of those for Th2-related cytokines. Conclusion: CVA differs from asthma and chronic cough in terms of Th1/Th2 cytokines and lung function and provides a reference for understanding the disease mechanism of early clinical progression of CVA to CA.

show abstract

“…Zhou and colleagues identified a link between the diversity of gut microbiota and the control of allergic asthma via modulation of immune responses. Specifically, the presence of the bacterial strains Ruminiclostridium 6 and Candidatus Arthromitus has been associated with balancing the Th1/Th2 and Treg/Th17 cell ratios in eosinophilic asthma cases (74). Zhou et al (2022) found 15 significant metabolism pathways, from gut microbiota, for eosinophilic asthma.…”

Section: Microbiome As a Risk Factor For Bronchial Asthmamentioning

confidence: 99%

“…Specifically, the presence of the bacterial strains Ruminiclostridium 6 and Candidatus Arthromitus has been associated with balancing the Th1/Th2 and Treg/Th17 cell ratios in eosinophilic asthma cases (74). Zhou et al (2022) found 15 significant metabolism pathways, from gut microbiota, for eosinophilic asthma. These included malate, I-dihydroorotate, 1,5-anhydro-d-sorbitol and imidazoleacetic acid which were linked with Th17/Treg.…”

Section: Microbiome As a Risk Factor For Bronchial Asthmamentioning

confidence: 99%

Exploring the immunopathology of type 2 inflammatory airway diseases

AlBloushi,

Al-Ahmad

2024

Front. Immunol.

View full text Add to dashboard Cite

Significant advancements have been achieved in understanding the roles of different immune cells, as well as cytokines and chemokines, in the pathogenesis of eosinophilic airway conditions. This review examines the pathogenesis of Chronic Rhinosinusitis with Nasal Polyps (CRSwNP), marked by complex immune dysregulation, with major contributions from type 2 inflammation and dysfunctional airway epithelium. The presence of eosinophils and the role of T-cell subsets, particularly an imbalance between Treg and Th17 cells, are crucial to the disease’s pathogenesis. The review also investigates the pathogenesis of eosinophilic asthma, a unique asthma subtype. It is characterized by inflammation and high eosinophil levels, with eosinophils playing a pivotal role in triggering type 2 inflammation. The immune response involves Th2 cells, eosinophils, and IgE, among others, all activated by genetic and environmental factors. The intricate interplay among these elements, chemokines, and innate lymphoid cells results in airway inflammation and hyper-responsiveness, contributing to the pathogenesis of eosinophilic asthma. Another scope of this review is the pathogenesis of Eosinophilic Granulomatosis with Polyangiitis (EGPA); a complex inflammatory disease that commonly affects the respiratory tract and small to medium-sized blood vessels. It is characterized by elevated eosinophil levels in blood and tissues. The pathogenesis involves the activation of adaptive immune responses by antigens leading to T and B cell activation and eosinophil stimulation, which causes tissue and vessel damage. On the other hand, Allergic Bronchopulmonary Aspergillosis (ABPA) is a hypersensitive response that occurs when the airways become colonized by aspergillus fungus, with the pathogenesis involving activation of Th2 immune responses, production of IgE antibodies, and eosinophilic action leading to bronchial inflammation and subsequent lung damage. This analysis scrutinizes how an imbalanced immune system contributes to these eosinophilic diseases. The understanding derived from this assessment can steer researchers toward designing new potential therapeutic targets for efficient control of these disorders.

show abstract

Plasma Metabolites and Gut Microbiota Are Associated With T cell Imbalance in BALB/c Model of Eosinophilic Asthma

Cited by 9 publications

References 36 publications

Guominkang formula alleviate inflammation in eosinophilic asthma by regulating immune balance of Th1/2 and Treg/Th17 cells

Guominkang formula alleviate inflammation in eosinophilic asthma by regulating immune balance of Th1/2 and Treg/Th17 cells

Study on the Relationship Between Bronchoalveolar Lavage Fluid Cell Count, Th1/Th2 Cytokines and Pulmonary Function in Patients with Cough Variant Asthma

Exploring the immunopathology of type 2 inflammatory airway diseases

Contact Info

Product

Resources

About