Plasma metabolic profiling of dairy cows affected with clinical ketosis using LC/MS technology

Li, Y; Xu, Chuchu; Xia, Cheng; Hy, Zhang; Sun, Lw; Gao, Yang

doi:10.1080/01652176.2014.962116

Cited by 50 publications

(49 citation statements)

References 31 publications

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“…Among the metabolites, 7 were amino acids. Similar to our study, they found decreased lysine and tryptophan in cattle showing clinical signs of ketosis 19 . Not surprisingly, they found a relationship between glucogenic amino acids such as arginine, valine, and glycine which are processed into phosphoenolpyruvate to synthesize glucose, and clinical ketosis 28 .…”

Section: Discussionsupporting

confidence: 92%

“…Not surprisingly, they found a relationship between glucogenic amino acids such as arginine, valine, and glycine which are processed into phosphoenolpyruvate to synthesize glucose, and clinical ketosis 28 . They also found a correlation between clinical ketosis and the levels of ketogenic amino acids such as leucine and lysine, which can be converted to ketones in the liver 29 , and amino acids that are both glucogenic and ketogenic such as tryptophan and isoleucine 19,30 . These studies support amino acid levels in the blood plasma being dynamic and reflecting the metabolic status of the animal.…”

Section: Discussionmentioning

confidence: 96%

“…In our study, we found lower levels of asparagine, lysine, glutamine, histidine, tryptophan, cysteine, and ornithine. Previously, the differential levels of amino acids in the blood of dairy cattle have been shown to correlate with ketosis 18,19,27 . One study looking at dairy cows with clinical ketosis found differential levels of 13 metabolites 19 .…”

Section: Discussionmentioning

confidence: 99%

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Plasma metabolomic profiles differ at the time of artificial insemination based on pregnancy outcome, in Bos taurus beef heifers

Phillips

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Kriese-Anderson

et al. 2018

Sci Rep

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Infertility remains the most prevalent reason for cattle being removed from production environments. We utilized metabolomic profiling to identify metabolites in the blood plasma that may be useful in identifying infertile heifers at the time of artificial insemination (AI). Prior to AI, phenotypic parameters including body condition, weight, and reproductive organ measurements were collected. These were determined not effective at differentiating between fertile and infertile heifers. Analysis of the resulting metabolomic profiles revealed 15 metabolites at significantly different levels (T-test P ≤ 0.05), with seven metabolites having a greater than 2-fold difference (T-test P ≤ 0.05, fold change ≥2, ROC-AUC ≥ 0.80) between infertile and fertile heifers. We further characterized the utility of using the levels of these metabolites in the blood plasma to discriminate between fertile and infertile heifers. Finally, we investigated the potential role inflammation may play by comparing the expression of inflammatory cytokines in the white blood cells of infertile heifers to that of fertile heifers. We found significantly higher expression in infertile heifers of the proinflammatory markers tumor necrosis factor alpha (TNFα), interleukin 6 (IL6), and the C-X-C motif chemokine 5 (CXCL5). Our work offers potentially valuable information regarding the diagnosis of fertility problems in heifers undergoing AI.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 96%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Plasma metabolomic profiles differ at the time of artificial insemination based on pregnancy outcome, in Bos taurus beef heifers

Phillips

Read

Kriese-Anderson

et al. 2018

Sci Rep

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“…In Holstein-Friesian cows, a milk glycerophosphocholine/phosphocholine ratio of 2.5 early in lactation was associated with a very low risk of developing ketosis (Klein et al 2012). Plasma metabolomic profiling was able to distinguish between lactating clinically normal cows, cows with subclinical ketosis, and cows with clinical ketosis (Zhang, Davis et al 2013;Li et al 2014;Sun et al 2014). Hepatic lipidosis could also be diagnosed using the plasma metabolome in transition Holstein-Friesian and Red-Holstein cows (Imhasly et al 2014) and early lactation Holstein-Friesian and Simmental cows (Humer et al 2016).…”

Section: Metabolome and Ketosismentioning

confidence: 99%

Metabolic health, the metabolome and reproduction in female cattle: a review

D’Occhio

Baruselli

Campanile

2019

Italian Journal of Animal Science

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Studies over the past 30 years have confirmed the important role of metabolic hormones and metabolic substrates in reproductive function in female cattle. The emergence of metabolomics is providing a deeper understanding of the role of specific metabolites, and clusters of metabolites, in reproduction and also health and disease. Dairy cows undergo major fluctuations in metabolic health and metabolomics is helping to better understand the changes in metabolite profiles associated with negative energy balance and ketosis. New knowledge that emerges from this work should lead to improved nutritional management of dairy cows. In reproduction, it is now possible to gain a metabolomic signature of ovarian follicular fluid and of developing embryos. This should likewise lead to improvements in both natural and assisted reproduction in cattle. Systems biology integrates genomics, transcriptomics, proteomics and metabolomics, and contributes to gaining an understanding of complex biological networks. HIGHLIGHTSMetabolic hormones and metabolic substrates have a major influence on reproduction in female cattle. Negative energy balance and ketosis are associated with changes in the systemic and liver metabolome in dairy cows. The metabolome of ovarian follicular fluid influences oocyte quality and embryo development. Systems biology integrates genomics, transcriptomics, proteomics and metabolomics, and provides a deeper understanding of complex biological networks. ARTICLE HISTORY

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“…Although the correlation between excessive fat intake and non-alcoholic fatty liver disease in humans remains controversial [4] , its prevalence has continued to increase concomitant with the epidemic of obesity. In addition, a severe negative energy balance (NEB) during the early lactation period, as a consequence of insufficient energy intake to sustain the high energy requirements for milk production, can also cause fatty liver, especially in dairy cows [5][6][7] , thereby resulting in major economic losses to the dairy industry [8,9] . Although liver biopsy is the gold standard for assessment of fatty liver disease, the procedure is invasive and only evaluates approximately 0.002% of the liver parenchyma [10] .…”

Section: Introductionmentioning

confidence: 99%

Negatif Enerji Dengesiyle Oluşturulmuş Koyun Yağlı Karaciğerinin Ultrasonografik Değerlendirmesi

Yang¹,

Xu²,

Cheng³

et al. 2017

Kafkas Univ Vet Fak Derg

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Fatty liver disease is a common liver disease in humans and animals alike, and macrofauna are considered an ideal model of fatty liver disease for ultrasonography (US)-based diagnostic research. Here, a model of fatty liver disease in lactating thin-tail sheep induced by negative energy balance was developed by restricted feeding. Ultrasonographic images were divided into the following fat classes according to liver triglyceride concentrations (ratio of triglyceride weight to wet liver weight): healthy liver, <2%; mild hepatic steatosis (HS), ≥2% to 5%; moderate HS, ≥5% to 10%; and severe HS, ≥10%. Characteristics of Ultrasonographic images according to HS severity were evaluated. As the results, mild to severe HS was detected by restricted feeding. The sensitivity of 5 MHz for diagnostic differentiation of healthy liver, and mild, moderate, and severe HS were 66.7%, 60.0%, 66.7%, and 82.5%, respectively, with a moderate κ statistic ranging from 0.667 to 0.735 among observers. The sensitivity of 3.5 MHz for diagnosis of healthy liver, and mild, moderate, and severe HS were 69.7%, 60.5%, 81.5%, and 75.4%, respectively, with moderate κ statistics ranging from 0.575 to 0.684 among observers. The sensitivities of 3.5 and 5 MHz for moderate to severe HS reached 97.4% and 98.2%, respectively. In summary, visualization at 3.5 and 5 MHz was ideal for diagnosis of HS except mild cases with established US diagnostic standards. Furthermore, with the established model, newer methods, such as image digitizing analysis, can be used for US-based diagnosis of fatty liver in further investigations. Keywords: Ultrasound, Fatty liver, Sheep, Negative energy balance Negatif Enerji Dengesiyle Oluşturulmuş Koyun Yağlı Karaciğerinin Ultrasonografik Değerlendirmesi ÖzetYağlı karaciğer hastalığı insanlarda ve benzer olarak hayvanlarda yaygın bir karaciğer hastalığı olup, makrofauna yağlı karaciğer hastalığının ultrasonografi (US)-temelli diagnostik araştırmaları için ideal bir modeldir. Bu çalışmada, laktasyondaki ince kuyruklu koyun ırkında negatif enerji dengesi ile yağlı karaciğer hastalığı modeli beslemenin kısıtlaması yoluyla oluşturulmuştur. Ultrasonografik görüntüler karaciğer trigliserid konsantrasyonu (yaş karaciğer ağırlığının trigliserid ağırlığına oranı) baz alınarak yağ sınıflarına ayrılmıştır; sağlıklı karaciğer, <2%; hafif hepatik steatozis (HS), ≥2% ile 5% arası; orta derecede HS, ≥5% ile 10% arası ve şiddetli HS, ≥10%. HS şiddetine göre ultrasonografik görüntülerin özellikleri değerlendirildi. Sonuç olarak kısıtlı besleme ile hafif ve orta derecelerde HS tespit edildi. Sağlıklı, hafif, orta ve şiddetli HS'nin diagnostik ayırımı için 5 MHz'nin hassasiyeti sırasıyla %66.7, %60.0, %66.7 ve %82.5 olarak belirlendi. Gözlemciler arasında ortalama κ istatistik 0.667 ile 0.735 arasında değişim gösterdi. Sağlıklı, hafif, orta ve şiddetli HS'nin diagnostik ayırımı için 3.5 MHz'nin hassasiyeti sırasıyla %69.7, %60.5, %81.5 ve %75.4 olarak belirlendi. Gözlemciler arasında ortalama κ istatistik 0.575 ile 0.684 arasında değiş...

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Plasma metabolic profiling of dairy cows affected with clinical ketosis using LC/MS technology

Abstract: Our results showed that the metabolic changes in cows with clinical ketosis involve complex metabolic networks and signal transduction. These results are important for future studies elucidating the pathogenesis, diagnosis, and prevention of clinical ketosis in dairy cows.

Cited by 50 publications

References 31 publications

Plasma metabolomic profiles differ at the time of artificial insemination based on pregnancy outcome, in Bos taurus beef heifers

Plasma metabolomic profiles differ at the time of artificial insemination based on pregnancy outcome, in Bos taurus beef heifers

Metabolic health, the metabolome and reproduction in female cattle: a review

Negatif Enerji Dengesiyle Oluşturulmuş Koyun Yağlı Karaciğerinin Ultrasonografik Değerlendirmesi

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