Plasma membrane proteomic analysis by TMT-PRM provides insight into mechanisms of aluminum resistance in tamba black soybean roots tips

Abstract

Aluminum (Al) toxicity in acid soil is a worldwide agricultural problem that inhibits crop growth and productivity. However, the signal pathways associated with Al tolerance in plants remain largely unclear. In this study, tandem mass tag (TMT)-based quantitative proteomic methods were used to identify the differentially expressed plasma membrane (PM) proteins in Tamba black soybean (TBS) root tips under Al stress. Data are available via ProteomeXchange with identifier PXD017160. In addition, parallel reaction monitoring (PRM) was used to verify the protein quantitative data. The results showed that 907 PM proteins were identified in Al-treated plants. Among them, compared to untreated plants, 90 proteins were differentially expressed (DEPs) with 46 up-regulated and 44 down-regulated (fold change > 1.3 or < 0.77, p < 0.05). Functional enrichment based on GO, KEGG and protein domain revealed that the DEPs were associated with membrane trafficking and transporters, modifying cell wall composition, defense response and signal transduction. In conclusion, our results highlight the involvement of GmMATE13, GmMATE75, GmMATE87 and H+-ATPase in Al-induced citrate secretion in PM of TBS roots, and ABC transporters and Ca2+ have been implicated in internal detoxification and signaling of Al, respectively. Importantly, our data provides six receptor-like protein kinases (RLKs) as candidate proteins for further investigating Al signal transmembrane mechanisms.

Integrated physiological, proteomic, and metabolomic analyses of pecan cultivar ‘Pawnee’ adaptation to salt stress

“…Various studies have shown that salt stress has a significant impact on both the photosynthetic structure and efficiency of plant leaves 29 , 30 . It has been found that the maximum quantum yield of Photosystem II (PSII; Fv/Fm) in salt-sensitive plants is significantly reduced during high salt stress 28 . For example, in the salt-sensitive sweet sorghum, salt stress was also found to significantly reduce P n, C i, and G s 31 .…”

“…Among them, the core DEPs and metabolites were determined through the MetaboAnalyst 5.0 online analysis platform ( https://www.metaboanalyst.ca/MetaboAnalyst/ ) to establish a regulatory network diagram. Furthermore, 20 proteins were chosen and quantified to verify the iTRAQ data by PRM analysis, with the detailed parameters for the liquid chromatography (LC) system and mass spectrometer taken from the relevant literature 28 . Three biological replicates of each group were performed.…”

Uncovering the transcriptional response of popcorn (Zea mays L. var. everta) under long-term aluminum toxicity

“…CASPs are involved in the formation and location of Casparian strips, nutrient uptake, and stress resistance 36 . Its expression is increased in cotton during cadmium stress 37 and in Tamba black soybean 38 during Al stress, thus affecting plant root growth. This interaction suggests the importance of ABC6 in the downstream regulation of CASP-like 5 under Al stress in popcorn roots.…”

Recent Advances in Understanding Mechanisms of Plant Tolerance and Response to Aluminum Toxicity

“…The sensing of Al stress by plants and the transduction of the Al signal to activate various downstream Al-tolerant strategies are fundamental frontier issues in plant sciences. Studies have shown that signaling cascades of Al-induced citrate exudation comprised heterotrimeric G-proteins, phospholipase C, inositol triphosphate, diacylglycerol, Ca 2+ , and protein kinases [2,6]. However, there are limited reports on Al transmembrane signal transduction.…”

“…Al mainly exists as aluminosilicates and oxides with non-phytotoxicity in neutral or slightly acidic soils. In acidic conditions (pH < 5.0), the mineral form of Al dissolves to release the soluble Al 3+ species, which can rapidly inhibit root elongation and further affect the uptake of water and nutrients, eventually resulting in nutritional deficiency and drought stress, which lead to severe loss of plant productivity [1,2]. Acidic soils occupy approximately 50% of potentially arable lands worldwide, most of which are distributed in Southwest Asia, Central Africa, and South America, as well as in Australia, eastern North America, and throughout Europe [3,4].…”