Plasma Membrane Proteins and Glycoproteins Induced by Human Cytomegalovirus Infection of Human Embryonic Fibroblasts

Sullivan-Tailyour, G.; Garnett, Helen M.

doi:10.1099/0022-1317-67-3-515

Cited by 4 publications

(1 citation statement)

References 29 publications

(18 reference statements)

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“…Because infected cells are known to express CMV antigens on their surface (Holtappels et al, 2002;Loh et al, 1991;Pereira et al, 1982;Stinski et al, 1979;Sullivan-Tailyour and Garnett, 1986;Tripathy et al, 2006), infected cells were expected to serve as suitable targets for MCMV antibody in sera from infected mice. This required exposure of target cells to MCMV reactive sera, and identification of antigen bound anti-CMV antibodies using PE-labeled mouse antiIgG 1 secondary antibody.…”

Section: Detection Of MCMV Antibodymentioning

confidence: 99%

A flow cytometry-based method for detecting antibody responses to murine cytomegalovirus infection

Bickerstaff

Zimmerman

Wing

et al. 2007

Journal of Virological Methods

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An assay based on target cells infected with green fluorescent protein labeled murine cytomegalovirus (GFP-MCMV) and dual color flow cytometry for detecting antibody to MCMV is described. After optimizing conditions for this technique, kinetics of anti-MCMV IgG antibody response were tested in susceptible (BALB/c) and resistant (C57BL/6) mouse strains following primary MCMV infection. Previously published antibody kinetics were confirmed in susceptible mice, with peak IgG response seen ~8 weeks after primary infection, decreasing by 20 weeks after infection. In contrast, MCMV resistant C57BL/6 mice showed significantly lower IgG antibody responses than susceptible mice. Although several techniques have been previously described to detect murine antibody responses to MCMV, including nuclear anti-complement immunofluorescence, viral immunoblotting, complement fixation, indirect immunofluorescence, indirect hemagglutination, and enzyme-liked immunosorbent assay techniques, these techniques are all time consuming and laborious. The technique presented is a simple time efficient alternative to detect previous MCMV antibody responses in experimentally infected mice.

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Section: Detection Of MCMV Antibodymentioning

confidence: 99%

A flow cytometry-based method for detecting antibody responses to murine cytomegalovirus infection

Bickerstaff

Zimmerman

Wing

et al. 2007

Journal of Virological Methods

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Virus‐specific serum IgG, IgM, and IgA antibodies in cytomegalovirus mononucleosis patients as determined by immunoblotting technique

Porath

Hanuka

Keynan

et al. 1987

Journal of Medical Virology

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The immune response to individual human cytomegalovirus (CMV) structural polypeptides was studied in paired sera from 15 adult CMV mononucleosis (CMV-MN) patients and healthy controls by immunoblotting technique (IB). IgM and IgG antibodies to at least 11 structural polypeptides with molecular weights of 28K, 49K, 55K, 57K, 66-70K, 82K, 87K, 110K, 150K, 205K, and 235K were detected in the patients' sera in the serum sample obtained in the acute phase of the disease. IgA antibodies to polypeptides with molecular weights of 66-70K, 82K, 110K, and 150K were also detected in these sera. In healthy seropositive adults, IgG antibodies with the same molecular weight polypeptides, excluding the 205K and 235K polypeptides, were detected as in convalescent CMV-MN patients. A prominent reactivity of IgM and IgA antibodies to the 66-70K and 150K polypeptides was noted in the acute sera from all the CMV-MN patients examined, but not in a number of late convalescent sera. The potential implications of these findings in the development of specific serological tests are discussed.

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Characterization of membrane antigens on human cytomegalovirus-infected fibroblasts recognized by human antibodies

Voort

Leij

The

1989

J Virol

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The antigens on the surface of human cytomegalovirus (HCMV)-infected fibroblasts which are recognized by human HCMV antibody-positive sera were characterized. Three HCMV-induced polypeptides, with apparent molecular masses of 53 to 63, 94, and 94 to 120 kilodaltons, were precipitated from '251-surface-labeled cell extracts with different sera obtained from healthy individuals. Renal transplant recipients who were suffering from active HCMV infections recognized the same set of antigens. By the use of monoclonal antibodies, these antigens were identified as polypeptides belonging to the gcI and gcIII families of HCMV glycoproteins.

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Plasma Membrane Proteins and Glycoproteins Induced by Human Cytomegalovirus Infection of Human Embryonic Fibroblasts

Cited by 4 publications

References 29 publications

A flow cytometry-based method for detecting antibody responses to murine cytomegalovirus infection

A flow cytometry-based method for detecting antibody responses to murine cytomegalovirus infection

Virus‐specific serum IgG, IgM, and IgA antibodies in cytomegalovirus mononucleosis patients as determined by immunoblotting technique

Characterization of membrane antigens on human cytomegalovirus-infected fibroblasts recognized by human antibodies

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