Plasma membrane order and fluidity are diversely triggered by elicitors of plant defence

Sándor, Roman; Der, Christophe; Grosjean, Kévin; Anca, Iulia; Noirot, Elodie; Leborgne-Castel, Nathalie; Lochman, Jan; Simon-Plas, Françoise; Gerbeau-Pissot, Patricia

doi:10.1093/jxb/erw284

Cited by 36 publications

(34 citation statements)

References 103 publications

(130 reference statements)

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“…Some MAMPs have also been reported to alter the plasma membrane state. Tobacco and Arabidopsis cells treated with flg22, oligogalacturonides or cryptogein induced an increase of lipid self-association in specific domains of the plasma membrane upon elicitation coinciding with the onset of plant defence signalling 67 , 68 . The same reports state that cryptogein enhances ROS production by recruiting plant sterols from the plasma membrane increasing its fluidity.…”

Section: Discussionmentioning

confidence: 99%

Synthetic Rhamnolipid Bolaforms trigger an innate immune response in Arabidopsis thaliana

Luzuriaga-Loaiza

Schellenberger

Gaetano

et al. 2018

Sci Rep

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Stimulation of plant innate immunity by natural and synthetic elicitors is a promising alternative to conventional pesticides for a more sustainable agriculture. Sugar-based bolaamphiphiles are known for their biocompatibility, biodegradability and low toxicity. In this work, we show that Synthetic Rhamnolipid Bolaforms (SRBs) that have been synthesized by green chemistry trigger Arabidopsis innate immunity. Using structure-function analysis, we demonstrate that SRBs, depending on the acyl chain length, differentially activate early and late immunity-related plant defense responses and provide local increase in resistance to plant pathogenic bacteria. Our biophysical data suggest that SRBs can interact with plant biomimetic plasma membrane and open the possibility of a lipid driven process for plant-triggered immunity by SRBs.

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Section: Discussionmentioning

confidence: 99%

Synthetic Rhamnolipid Bolaforms trigger an innate immune response in Arabidopsis thaliana

Luzuriaga-Loaiza

Schellenberger

Gaetano

et al. 2018

Sci Rep

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“…A plausible explanation for reduced lateral mobility is the well-established formation or stabilisation of receptor (hetero-)oligomers within the PM for activation of signal transduction as previously reported for FLS2 and BRI1 (Chinchilla et al, 2007; Somssich et al, 2015; Wang et al, 2008; Bücherl et al, 2013). The low lateral mobility of PM proteins in general and the additional confinement in response to ligands also suggests a ligand-independent pre-organisation of receptors and/or signalling units (Martinière et al, 2012; Abulrob et al, 2010; Sandor et al, 2016). …”

Section: Discussionmentioning

confidence: 99%

Plant immune and growth receptors share common signalling components but localise to distinct plasma membrane nanodomains

Bücherl

Jarsch

Schudoma

et al. 2017

eLife

198

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Cell surface receptors govern a multitude of signalling pathways in multicellular organisms. In plants, prominent examples are the receptor kinases FLS2 and BRI1, which activate immunity and steroid-mediated growth, respectively. Intriguingly, despite inducing distinct signalling outputs, both receptors employ common downstream signalling components, which exist in plasma membrane (PM)-localised protein complexes. An important question is thus how these receptor complexes maintain signalling specificity. Live-cell imaging revealed that FLS2 and BRI1 form PM nanoclusters. Using single-particle tracking we could discriminate both cluster populations and we observed spatiotemporal separation between immune and growth signalling platforms. This finding was confirmed by visualising FLS2 and BRI1 within distinct PM nanodomains marked by specific remorin proteins and differential co-localisation with the cytoskeleton. Our results thus suggest that signalling specificity between these pathways may be explained by the spatial separation of FLS2 and BRI1 with their associated signalling components within dedicated PM nanodomains.DOI: http://dx.doi.org/10.7554/eLife.25114.001

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“…These chemical effects could translate into changes in membrane biophysical properties, such as viscosity or curvature, and act on membrane internalization (Eichenberger et al, 1982;Bruch and Thayer, 1983). In addition, it cannot be excluded that ROS accumulation triggers formation of ordered microdomains at the PM to promote endocytosis, as was described in cells exposed to pathogen elicitors (Sandor et al, 2016).…”

Section: The Two Ros-producing Pathways Contribute In Synergy To Lipimentioning

confidence: 97%

Osmotic Stress Activates Two Reactive Oxygen Species Pathways with Distinct Effects on Protein Nanodomains and Diffusion

et al. 2019

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Y.J.); 0000-0002-4255-6440 (C.M.).Physiological acclimation of plants to an everchanging environment is governed by complex combinatorial signaling networks that perceive and transduce various abiotic and biotic stimuli. Reactive oxygen species (ROS) serve as one of the second messengers in plant responses to hyperosmotic stress. The molecular bases of ROS production and the primary cellular processes that they target were investigated in the Arabidopsis (Arabidopsis thaliana) root. Combined pharmacological and genetic approaches showed that the RESPIRATORY BURST OXIDASE HOMOLOG (RBOH) pathway and an additional pathway involving apoplastic ascorbate and iron can account for ROS production upon hyperosmotic stimulation. The two pathways determine synergistically the rate of membrane internalization, within minutes after activation. Live superresolution microscopy revealed at single-molecule scale how ROS control specific diffusion and nano-organization of membrane cargo proteins. In particular, ROS generated by RBOHs initiated clustering of the PLASMA MEMBRANE INTRINSIC PROTEIN2;1 aquaporin and its removal from the plasma membrane. This process is contributed to by clathrin-mediated endocytosis, with a positive role of RBOH-dependent ROS, specifically under hyperosmotic stress.

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Plasma membrane order and fluidity are diversely triggered by elicitors of plant defence

Abstract: HighlightThe ROS-dependent increase of plasma membrane order is a generic event triggered by elicitors of plant defence, whereas fluidity enhancement is specifically triggered by cryptogein, a sterol-carrier elicitin

Cited by 36 publications

References 103 publications

Synthetic Rhamnolipid Bolaforms trigger an innate immune response in Arabidopsis thaliana

Synthetic Rhamnolipid Bolaforms trigger an innate immune response in Arabidopsis thaliana

Plant immune and growth receptors share common signalling components but localise to distinct plasma membrane nanodomains

Osmotic Stress Activates Two Reactive Oxygen Species Pathways with Distinct Effects on Protein Nanodomains and Diffusion

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