Plasma membrane glycoproteins of cultured rheumatoid synovial fibroblasts

Kouri, Timo; Vuorio, Eero

doi:10.1007/bf02032172

Cited by 8 publications

(6 citation statements)

References 21 publications

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“…Here we were unable to determine a specific protein pattern in rheumatoid arthritis. This is in line with results cited earlier (Kouri & Vuorio, 1983;Kouri et al, 1984;Trapani et al, 1984;Glennas et al, 1985;Inman et al, 1985;Mairs & Beeley, 1985), which were based on conventional 2D-electrophoresis and previous results based on a microelectrophoresis technique (Fritz et al, 1990). When the patients were grouped not on the basis of the clinical diagnosis but on that of the histomorphological synovitis pattern, a statistically significant correlation was found between the synovitis type and the protein spot number.…”

Section: Discussionsupporting

confidence: 90%

“…In rheumatology there are so far only rare examples of its application. These mainly failed to demonstrate a rheumatoid arthritis-specific protein pattern (Kouri & Vuorio, 1983;Kouri et al, 1984;Trapani et a]., 1984;Glennas et al, 1985;Mairs & Beeley, 1985;Inman et aI., 1985;Fritz et al, 1990). In this paper we describe a variant of 2D-electrophoresis which permits the analysis of proteins occurring in the synovial membrane without prior tissue homogenization.…”

Section: Discussionmentioning

confidence: 95%

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Two-dimensional cryostat section electrophoresis: a novel method and its application to the diseased synovial membrane

et al. 1994

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A two-dimensional electrophoresis technique for analysing sections of human tissue is described. Cryostat sections, 10 microns thick, are placed on an isoelectric focusing gel and then transferred to an SDS gel in the second dimension. The protein pattern is visualized by silver staining and is thought to represent soluble proteins. The silver-stained proteins were found to be both reproducible and, to the extent tested, organ-specific. This method was used to analyse 43 synovial membranes from patients suffering from rheumatoid arthritis or degenerative joint diseases. The analysis did not reveal any specific protein pattern for rheumatoid arthritis. The protein spot number was not related to the cause of arthritis. However, the total protein spot number was related to the histomorphological synovitis type, with those exhibiting either an exudative or proliferative synovitis pattern possessing significantly higher protein spot numbers than those specimens exhibiting a sero-fibrous or lympho-plasmacytic pattern of synovitis.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 95%

Two-dimensional cryostat section electrophoresis: a novel method and its application to the diseased synovial membrane

et al. 1994

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“…3). The same results have been obtained for their galactose/galactosamine-containing surface glycoproteins [13] and their lentil lectin-bound glycoproteins [12].…”

Section: Discussionsupporting

confidence: 81%

Surface Membrane Glycoproteins of Macrophage‐like Synovial Cells from Rheumatoid and Control Patients

1984

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Synovial cells were prepared by enzyme digestion and Percoll gradient centrifugation of rheumatoid arthritis (RA) synovial specimens or by trypsin-rinsing of non-inflammatory cadaver joints. Most (70-80%) of the cells from RA patients were OKIa -positive macrophage-like cells, 10-20% other OKIa -positive cells, and about 10% fibroblastic cells, whereas 90% of the normal synovial cells were OKIa -positive macrophage-like cells and the rest fibroblasts. These adherent synovial cells were compared with fibroblastic synovial cells obtained by sequential passaging of explanted dividing cells. Periodate-[3H]borohydride labelling followed by SDS-gradient gel electrophoresis demonstrated similar sialo-glycoprotein patterns in both the adherent synovial cells and synovial fibroblasts. The molecular weights of the main surface glycoproteins resembled closely those of skin fibroblasts but not those of peripheral blood monocytes. RA samples showed inconsistent heterogeneity. The results indicate either that all synovial cells possess a similar basic structure or that macrophages of peripheral blood origin express fibroblastic contact glycoproteins when settling down into synovium.

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“…There have only been a few reports concerning the application of electrophoretic techniques to studies of samples taken from RA patients or from patients with related diseases [49][50][51][52][53][54][55]. Studying a group of RA patients, Kouri et al [49,50] did not find disease-related differences in the 2D polypeptide pattern of 3 s S methionine-labelled plasma membrane and rough endoplasmatic reticulum extracts obtained from lysates of synovial cell cultures derived from RA or non-RA patients.…”

Section: Discussionmentioning

confidence: 96%

“…Studying a group of RA patients, Kouri et al [49,50] did not find disease-related differences in the 2D polypeptide pattern of 3 s S methionine-labelled plasma membrane and rough endoplasmatic reticulum extracts obtained from lysates of synovial cell cultures derived from RA or non-RA patients. Using 1D electrophoresis, Inman et al [51] were also unable to demonstrate disease-specific protein patterns.…”

Section: Discussionmentioning

confidence: 97%

Two-dimensional electrophoresis of synovial tissue, synovial fluid and serum in patients with rheumatoid arthritis and related diseases

et al. 1990

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Using the technique of two-dimensional (2D) electrophoresis with consecutive silver staining, we investigated samples of serum, synovial fluid and synovial tissue obtained from 19 patients suffering from rheumatoid arthritis (RA) or non-RA arthritis. From these experiments we have drawn the following conclusions. 2D electrophoresis of serum, synovial fluid and synovial tissue extracts taken from patients suffering from joint diseases is a reproducible method. Repeated runs of the same sample reveal an essentially constant protein spot pattern. The time period between surgery and tissue preparation did not influence the number of protein spots when less than 15 h was involved. The protein spot number is always lower in synovial fluid than in either synovial tissue or serum in RA and non-RA patients. The mean value for the number of spots is 68 for the inflamed tissue irrespective of the cause of arthritis (RA and non-RA group taken together) and 47 for the control group. This difference is significant. We were able to definitely identify 7 spots in the tissue extract. We did not find RA-specific protein spots in either serum, synovial fluid or tissue extracts from the synovial membrane. The only significant difference between RA patients and either non-RA or control group patients concerning the protein spot pattern is the increased size of the immunoglobulin spot (mainly IgG) in RA. In addition, we discuss possible reasons for failure of the 2D electrophoresis technique to detect disease-specific protein patterns.

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Plasma membrane glycoproteins of cultured rheumatoid synovial fibroblasts

Cited by 8 publications

References 21 publications

Two-dimensional cryostat section electrophoresis: a novel method and its application to the diseased synovial membrane

Two-dimensional cryostat section electrophoresis: a novel method and its application to the diseased synovial membrane

Surface Membrane Glycoproteins of Macrophage‐like Synovial Cells from Rheumatoid and Control Patients

Two-dimensional electrophoresis of synovial tissue, synovial fluid and serum in patients with rheumatoid arthritis and related diseases

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