Plasma-membrane-associated immunoglobulins and other polypeptides of pig mesenteric node lymphocytes

Chavin, Stephen I.; Sm, Johnson; Holliman, Andrew

doi:10.1042/bj1480417

Cited by 11 publications

(5 citation statements)

References 45 publications

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“…The remaining lipid was made up of ceramides, glycosphingolipids and gangliosides. The phospho-lipid composition ( Table 2) resembled closely that reported for the plasma membrane of pig mesenteric lymph-node lymphocytes (Chavin et al, 1975).…”

Section: Discussionsupporting

confidence: 78%

Lipid composition of lymphocyte plasma membrane from pig mesenteric lymph node

Levis

Evangelatos

Crumpton³

1976

Biochemical Journal

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1. The lipid fraction of the plasma membrane of pig mesenteric lymph-node lymphocytes contained primarily (94%) neutral lipids and phospholipids in about equal weights. The remianing lipid comprised glycosphingolipids (1.8%), gangliosides (o.27%)and probably ceramides (1.3%). The major phospholipid was phosphatidylcholine (46% of the total), and mono- and tri-hexosylceramides accounted for 72% of the glycosphingolipids. Haematoside was distributed between the glycosphingolipid and ganglioside fractions. The major ganglioside was monosialoganglioside. About 90% of the sialic acid was N-glycollylated. 2. A comparision of the lipid composition of the plasma-membrane fraction with that of the initial lymph-node homogenate showed that the purified membrane contained increased proportions of phospholipids, especially sphingomyelin, glycosphingolipids and gangliosides. 3. Fatty acid analyses showed that the membrane phosphatidylcholine was rich in palmitic acid, that the sphingomeyelin and phosphatidylethanolamine were high in myristic acid and that the glycosphingolipids were rich in oleic acid.

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Section: Discussionsupporting

confidence: 78%

Lipid composition of lymphocyte plasma membrane from pig mesenteric lymph node

Levis

Evangelatos

Crumpton³

1976

Biochemical Journal

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“…According to various morphological, biochemical, and immunological criteria, the plasma membrane fraction was not contaminated by significant amounts of endoplasmic reticulum or golgi membrane (Allan and Crumpton, 1970). Various arguments suggest that the purified plasma membrane fraction was derived primarily from small lymphocytes (Allan and Crumpton, 1970;Chavin et al, 1975). This interpretation is endorsed by the observation that the polypeptide composition of the plasma membrane preparations, as revealed by polyacrylamide gel electrophoresis in sodium dodecyl sulfate, did not differ significantly from that of plasma membrane prepared from suspensions of pig mesenteric lymph node lymphocytes (B. H. Barber, M. J. Crumpton, and F. S. Walsh, unpublished observations).…”

Section: Methodsmentioning

confidence: 99%

Preparation of inside-out vesicles of pig lymphocyte plasma membrane

Walsh¹,

Barber²,

Crumpton³

1976

Biochemistry

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Between 30 and 50% of pig lymphocyte plasma membrane vesicles were not bound by concanavalin A (Con A)-Sepharose. Various results suggest that the Con A-unretarded fraction represents "inside-out" membrane vesicles. First, an alternative cell surface ligand, anti-lymphocytic serum, gave a similar fractionation to Con A. Second, lack of binding by Con A was not due to lack of carbohydrate or to masking of carbohydrate by extraneous protein, because the unfractionated membrane and the unretarded fraction had similar carbohydrate and polypeptide compositions. Third although the carbohydrate of the unretarded membrane vesicles was accessible to 125I-labelled Con A and to release by soluble trypsin, it was not accessible to ferritin-Con A or trypsin-Sepharose. Fourth, antisera against the external surface of the Con A-unretarded vesicles strongly agglutinated the unretarded membrane, but caused negligible agglutination of whole lymphocytes. When attached to Sepharose these antisera bound all of the Con A-unretarded fraction, but failed to bind the membrane that adhered to Con A-Sepharose.

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“…Membranes were prepared from intact mesenteric lymph nodes of young pigs by the method of Allan & Crumpton (1970) as modified in our laboratory (Chavin et al, 1975). dissolve the membranes.…”

Section: Materials and Methods Preparation Ofmembranesmentioning

confidence: 99%

“…Polyacrylamide-gel electrophoresis (Neville & Glossmann, 1971) and immunological procedures (Heremans, 1971) were performed as reported (Chavin et al, 1975). Protein was determined by the method of Lowry et al (1951), except that Mg2+ was included in blanks and standard bovine serum albumin solutions when the unknowns contained this cation.…”

Section: Other Methodsmentioning

confidence: 99%