Plasma mediated collagen‐I‐coating of metal implant materials to improve biocompatibility

Abstract

This study describes the collagen-I coating of titanium and steel implants via cold low-pressure gas plasma treatment. To analyze the coatings in terms of biocompatibility osteoblast-like osteosarcoma cells and human leukocytes were cultivated on the metal surfaces. Two different implant materials were assessed (Ti6Al4V, X2CrNiMo18) and four different surface properties were evaluated: (a) plasma pretreated and collagen-I coated implant materials; (b) collagen-I dip-coated without plasma pretreatment; (c) plasma treated but not collagen-I coated; (d) standard implant materials served as control. The different coating characteristics were analyzed by scanning electron microscopy (SEM). For adhesion and viability tests calcein-AM staining of the cells and Alamar blue assays were performed. The quantitative analysis was conducted by computer assisted microfluorophotography and spectrometer measurements. SEM analysis revealed that stable collagen-I coatings could not be achieved on the dip-coated steel and titanium alloys. Only due to pretreatment with low-pressure gas plasma a robust deposition of collagen I on the surface could be achieved. The cell viability and cell attachment rate on the plasma pretreated, collagen coated surfaces was significantly (p < 0.017) increased compared to the non coated surfaces. Gas plasma treatment is a feasible method for the deposition of proteins on metal implant materials resulting in an improved biocompatibility in vitro. (c) 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010.

Immobilization of epidermal growth factor on titanium and stainless steel surfaces via dopamine treatment

“…In addition to surface modifications aimed at preventing biofouling [1,5,6], extracellular matrix, active peptides, or derivatives have been immobilized on metals to promote cell adhesion [7][8][9][10][11][12][13].…”

In vitro response of human peripheral blood mononuclear cells to AISI 316L austenitic stainless steel subjected to nitriding and collagen coating treatments

“…Half of the samples (untreated or nitrided) were collagen coated, following the guidelines of Hauser et al [12]. Collagen type I from rat tail 4 mg/ml in 20 mM acetic acid (BD Biosciences, Bedford, MA) was diluted 1:8 with sterilized deionized water to 0.5 mg/ml end concentration.…”

“…Another adopted strategy is the coating of medical devices with more biocompatible materials. Hauser et al [12] improved the biocompatibility of titanium and stainless steel alloys by plasma mediated collagen-Icoating. As reported in an our previous paper [13], collagen coating can be also promoted by low temperature nitriding treatment of AISI 316L austenitic stainless steel, and this collagen protection may be favourable for endothelial cell proliferation and for the control of metalloproteinase (MMP)-2 release.…”

Materials in Fracture Fixation

“…PTH-and bisphosphonate-coated stainless steel screws increased pullout strength and energy as well as fixation. 58,59 Plates that elute recombinant human BMP 2 were able to induce new bone formation and heal large segmental bone defects. [60][61][62] Lastly, strontium-eluting implants are capable of simultaneously stimulating bone formation and inhibiting bone resorption.…”