Plasma markers of COVID-19 severity: a pilot study

Beimdiek, Julia; Janciauskiene, Sabina; Wrenger, Sabine; Volland, Sonja; Roży, Adriana; Fuge, Jan; Olejnicka, Beata; Pink, Isabell; Illig, Thomas; Popov, Alexander; Chorostowska, Joanna; Buettner, Falk F. R.; Welte, Tobias

doi:10.1186/s12931-022-02272-7

Cited by 13 publications

(16 citation statements)

References 66 publications

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“…Beimdiek et al reported an elevation in the level of sialylated bi-antennary glycans supporting our results, although they have also found increased level of oligomannose structures, which was not significantly altered and rather decreased in our results. [ 16 ]. Higher sialylation in response to influenza vaccine was also documented suggesting that glycosylation alterations can improve the identification of responders [ 19 ].…”

Section: Resultsmentioning

confidence: 99%

“…The changes in serum N-glycosylation have also been identified in several infectious diseases including tuberculosis, HIV, influenza, ebola and viral hepatitis [ 14 ]. Recent studies suggest that serum glycosylation can be significantly altered in patients after SARS-CoV-2 infection, and the analysis of serum N-glycome might be significant in the surveillance of COVID-19 [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

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The Alterations of Serum N-glycome in Response to SARS-CoV-2 Vaccination

Dojcsák

Kardos

Szabó

et al. 2023

IJMS

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The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has caused a global concern since its outbreak in 2019, with one of the main solutions being vaccination. Altered glycosylation has been described in patients after SARS-CoV-2 infection, while the effect of vaccination on serum glycoproteins remained unexplored. In this study, total serum glycosylation was analyzed in patients after SARS-CoV-2 infection and/or mRNA vaccination in order to identify potential glycosylation-based alterations. Enzyme-linked immunosorbent assay was applied to identify post-COVID-19 and post-Vaccinated patients and rule out potential outliers. Serum samples were deglycosylated by PNGase F digestion, and the released glycans were fluorescently derivatized using procainamide labeling. Solid-phase extraction was used to purify the labeled glycans followed by the analysis of hydrophilic-interaction liquid chromatography with fluorescence and mass-spectrometric detection. Alterations of serum N-glycome in response to SARS-CoV-2 infection and mRNA vaccination were revealed by linear discriminant analysis.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

The Alterations of Serum N-glycome in Response to SARS-CoV-2 Vaccination

Dojcsák

Kardos

Szabó

et al. 2023

IJMS

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“…Several proteins linked to the acute phase response, such as CRP , SERPINA3 , SAA1 and SAA2 , have already been shown as increased in sera of severe COVID-19 patients, through an MS based Data Independent Analysis by Lee et al [ 32 ]. ORM1 and HP were also highlighted as altered depending on the severity of the patients by Shen et al in serum [ 33 ] and by Beimdiek J et al in plasma, respectively [ 34 ]. The modulation and the polymorphism of the human leukocyte antigen (HLA) play a key role in the immune response, and its variants could affect COVID-19 progression and severity [ 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Plasma Proteomic Variables Related to COVID-19 Severity: An Untargeted nLC-MS/MS Investigation

Pagani

Chinello

Risca

et al. 2023

IJMS

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Severe Acute Respiratory Syndrome Coronavirus-2 (SARS-CoV-2) infection leads to a wide range of clinical manifestations and determines the need for personalized and precision medicine. To better understand the biological determinants of this heterogeneity, we explored the plasma proteome of 43 COVID-19 patients with different outcomes by an untargeted liquid chromatography-mass spectrometry approach. The comparison between asymptomatic or pauci-symptomatic subjects (MILDs), and hospitalised patients in need of oxygen support therapy (SEVEREs) highlighted 29 proteins emerged as differentially expressed: 12 overexpressed in MILDs and 17 in SEVEREs. Moreover, a supervised analysis based on a decision-tree recognised three proteins (Fetuin-A, Ig lambda-2chain-C-region, Vitronectin) that are able to robustly discriminate between the two classes independently from the infection stage. In silico functional annotation of the 29 deregulated proteins pinpointed several functions possibly related to the severity; no pathway was associated exclusively to MILDs, while several only to SEVEREs, and some associated to both MILDs and SEVEREs; SARS-CoV-2 signalling pathway was significantly enriched by proteins up-expressed in SEVEREs (SAA1/2, CRP, HP, LRG1) and in MILDs (GSN, HRG). In conclusion, our analysis could provide key information for ‘proteomically’ defining possible upstream mechanisms and mediators triggering or limiting the domino effect of the immune-related response and characterizing severe exacerbations.

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“…Cell death, In ammation, N-glycosylation, thrombosis have been studied and among the critical COVID-19 biomarkers that illustrate the case severity [45].…”

Section: Discussionmentioning

confidence: 99%

Accumulation of oxysterols in the erythrocytes of COVID-19 patients as a biomarker for case severity

Khedr

Khayat

Khayyat

et al. 2023

Preprint

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Background: Due to the high risk of COVID-19 patients to the formation of thrombosis in the circulating blood, atherosclerosis, and myocardial infarction, it was necessary to study the lipidomic of the erythrocytes. The aim of this work was to analyze the pathogenic oxysterols and acylcarnitines in the erythrocyte’s homogenate of COVID-19 patients and to estimate the case severity from the level of oxysterols. Methods: A linear ion trap mass spectrometry coupled with high-performance liquid chromatography was used to investigate the extract of erythrocytes homogenate. The toxic biomarkers that primarily induce the generation of dead red blood cells, were characterized, and quantified in the erythrocytes of COVID-19 patients and matched with healthy volunteers. Results: A total of 30 patients and 30 healthy volunteers were enrolled. The concentration of five oxysterols and six acylcarnitines in the erythrocyte’s homogenate of COVID-19 patients was significantly upregulated matching with healthy subjects at p <0.05. The average total concentration of oxysterols was 23.36 ± 13.47 μg/mL in the erythrocytes of COVID-19 patients, while samples of healthy volunteers showed a total concentration of 4.92 ± 1.61 μg/mL. The average concentration level of 7-ketocholesterol and 4-cholestenone in the COVID-19 patients was higher by five and ten-fold compared to the healthy subjects. Also, the average concentration of acylcarnitines in the erythrocyte's homogenate of COVID-19 patients was high by 2-to-4-fold in comparison with the healthy volunteers. Conclusions: The abnormally high levels of oxysterols and acylcarnitines found in the erythrocytes of COVID-19 patients were associated with the severity of the case's complications and substantial risk of thrombosis. The concentration of oxysterols in the erythrocyte homogenate could be useful as a diagnostic biomarker to stand on the COVID-19 case severity.

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Plasma markers of COVID-19 severity: a pilot study

Cited by 13 publications

References 66 publications

The Alterations of Serum N-glycome in Response to SARS-CoV-2 Vaccination

The Alterations of Serum N-glycome in Response to SARS-CoV-2 Vaccination

Plasma Proteomic Variables Related to COVID-19 Severity: An Untargeted nLC-MS/MS Investigation

Accumulation of oxysterols in the erythrocytes of COVID-19 patients as a biomarker for case severity

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