Plasma Lipid Profile Reveals Plasmalogens as Potential Biomarkers for Colon Cancer Screening

Fernandes, Anna Maria A. P.; Messias, Márcia Cristina Fernandes; Duarte, Gustavo Henrique Bueno; Santis, Gabrielle Kristine Doratiotto; Mecatti, Giovana Colozza; Porcari, Andréia M.; Murgu, Michael; Simionato, Ana Valéria Colnaghi; Rocha, Thalita; Carvalho, Patrícia O.

doi:10.3390/metabo10060262

Cited by 15 publications

(12 citation statements)

References 61 publications

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“…Following a method previously published by our group [ 47 ], untargeted LC-MS analysis was performed using an ACQUITY UPLC coupled to a XEVO-G2XS QTOF mass spectrometer (Waters, Manchester, UK). Liquid chromatography was performed using an Acquity UPLC CSHC18 column (2.1 × 100 mm, 1.7 mm, Waters).…”

Section: Methodsmentioning

confidence: 99%

Potential Lipid Signatures for Diagnosis and Prognosis of Sepsis and Systemic Inflammatory Response Syndrome

et al. 2020

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Systemic inflammatory response syndrome (SIRS) and sepsis are two conditions which are difficult to differentiate clinically and which are strongly impacted for prompt intervention. This study identified potential lipid signatures that are able to differentiate SIRS from sepsis and to predict prognosis. Forty-two patients, including 21 patients with sepsis and 21 patients with SIRS, were involved in the study. Liquid chromatography coupled to mass spectrometry and multivariate statistical methods were used to determine lipids present in patient plasma. The obtained lipid signatures revealed 355 features for the negative ion mode and 297 for the positive ion mode, which were relevant for differential diagnosis of sepsis and SIRS. These lipids were also tested as prognosis predictors. Lastly, L-octanoylcarnitine was found to be the most promising lipid signature for both the diagnosis and prognosis of critically ill patients, with accuracies of 75% for both purposes. In short, we presented the determination of lipid signatures as a potential tool for differential diagnosis of sepsis and SIRS and prognosis of these patients.

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Section: Methodsmentioning

confidence: 99%

Potential Lipid Signatures for Diagnosis and Prognosis of Sepsis and Systemic Inflammatory Response Syndrome

et al. 2020

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Section: Significance Of Studying the Effect Of Plasmalogen On Membrane Morphologymentioning

confidence: 99%

“…First, plasma plasmalogen levels could be used as a biomarker to indicate the onset of cellular dysfunction and monitor the rate and extent of the deterioration or improvement ( Fernandes et al, 2020 ). Detecting plasmalogens in the blood, for example, would be an advantage over other methods of detection ( Fernandes et al, 2020 ). Second, restoring plasmalogen levels (by oral supplements for example) at the appropriate intervention time could potentially delay or prevent the onset of cellular dysfunction that is associated with neurodegenerative and/or age-related diseases.…”

Section: Significance Of Studying the Effect Of Plasmalogen On Membrane Morphologymentioning

confidence: 99%

Potential Role of Plasmalogens in the Modulation of Biomembrane Morphology

Almsherqi

2021

Front. Cell Dev. Biol.

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Plasmalogens are a subclass of cell membrane glycerophospholipids that typically include vinyl- ether bond at the sn-1 position and polyunsaturated fatty acid at the sn-2 position. They are highly abundant in the neuronal, immune, and cardiovascular cell membranes. Despite the abundance of plasmalogens in a plethora of cells, tissues, and organs, the role of plasmalogens remains unclear. Plasmalogens are required for the proper function of integral membrane proteins, lipid rafts, cell signaling, and differentiation. More importantly, plasmalogens play a crucial role in the cell as an endogenous antioxidant that protects the cell membrane components such as phospholipids, unsaturated fatty acids, and lipoproteins from oxidative stress. The incorporation of vinyl-ether linked with alkyl chains in phospholipids alter the physicochemical properties (e.g., the hydrophilicity of the headgroup), packing density, and conformational order of the phospholipids within the biomembranes. Thus, plasmalogens play a significant role in determining the physical and chemical properties of the biomembrane such as its fluidity, thickness, and lateral pressure of the biomembrane. Insights on the important structural and functional properties of plasmalogens may help us to understand the molecular mechanism of membrane transformation, vesicle formation, and vesicular fusion, especially at the synaptic vesicles where plasmalogens are rich and essential for neuronal function. Although many aspects of plasmalogen phospholipid involvement in membrane transformation identified through in vitro experiments and membrane mimic systems, remain to be confirmed in vivo, the compiled data show many intriguing properties of vinyl-ether bonded lipids that may play a significant role in the structural and morphological changes of the biomembranes. In this review, we present the current limited knowledge of the emerging potential role of plasmalogens as a modulator of the biomembrane morphology.

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“…Epigenetic modification, such as MLH1 hypermethylation that results in a loss of mismatch repair methylation change, has been suggested as a longitudinal biomarker for CRC [ 11 ]. Moreover, lipid peroxidation and altered metabolites are currently considered a hallmark characteristic of CRC [ 12 , 13 , 14 ]. Therefore, genetic, epigenetic, and lipidic biomarkers are needed to provide new insights into CRC risk, development, and progression.…”

Section: Introductionmentioning

confidence: 99%

Metabolic Evidence Rather Than Amounts of Red or Processed Meat as a Risk on Korean Colorectal Cancer

Kim

Lee²,

Kim

et al. 2021

Metabolites

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The incidence of colorectal cancer (CRC) has increased in Korea, a newly-industrialized Asian country, with the dramatic increase of meat intake. To assess the risks of red or processed meat consumption on CRC, we performed a case-control study with biological monitoring of urinary1-OHP, PhIP, and MeIQx for the meat exposure; dG-C8 MeIQx and dG-C8 PhIP for HCA-induced DNA adducts; and homocysteine and CRP in blood as well as malondialdehyde (MDA) and 31fatty acids in urine for inflammation and lipid alteration. We further analyzed global DNA methylation and expression of 15 CRC-related genes. As a result, the consumption of red or processed meat was not higher in the cases than in the controls. However, urinary MeIQx and PhIP were associated with the intake of red meat and urinary 1-OHP. MDA and multiple fatty acids were related to the exposure biomarkers. Most of the 31 fatty acids and multiple saturated fatty acids were higher in the cases than in the controls. Finally, the cases showed upregulation of PTGS2, which is related to pro-inflammatory fatty acids. This study describes indirect mechanisms of CRC via lipid alteration with a series of processes including exposure to red meat, alteration of fatty acids, and relevant gene expression.

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Plasma Lipid Profile Reveals Plasmalogens as Potential Biomarkers for Colon Cancer Screening

Cited by 15 publications

References 61 publications

Potential Lipid Signatures for Diagnosis and Prognosis of Sepsis and Systemic Inflammatory Response Syndrome

Potential Lipid Signatures for Diagnosis and Prognosis of Sepsis and Systemic Inflammatory Response Syndrome

Potential Role of Plasmalogens in the Modulation of Biomembrane Morphology

Metabolic Evidence Rather Than Amounts of Red or Processed Meat as a Risk on Korean Colorectal Cancer

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