Plasma lipid peroxidation and antioxidant levels in patients with rheumatoid arthritis

Kamanlı, Ayhan; Nazıroğlu, Mustafa; Aydılek, Nurettın; Hacıevlıyagil, Cengiz

doi:10.1002/cbf.1055

Cited by 158 publications

(139 citation statements)

References 20 publications

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“…However our study is in line with [13,22] who have reported increased SOD levels in RA patients. Mazetti et al [13] have reported higher serum copper/Zn superoxide in patients with RA.…”

Section: Mdasupporting

confidence: 93%

“…Lipid peroxides are generated at the site of tissue injury due to inflammation and diffuses into blood and can be estimated in serum or plasma [20]. Studies [21][22][23][24] have reported raised levels of MDA in the serum, plasma and erythrocytes of RA patients. In our study SOD levels are highly increased (Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…They showed reduced activities for catalase and glutathione reductase ( Table 1). The GR activity was positively correlated to MDA, SOD and ALP ( Reactive oxygen species and oxidative stress have a role in the pathogenesis of RA [22]. Free radicals and other reactive species play an important role of super oxidant leading to oxidation of biomolecules like proteins, amino acids, lipids and DNA [26], which are ultimately responsible for cell injury and death [27].…”

Section: Resultsmentioning

confidence: 99%

“…Measurement of MDA is widely used as an indicator of LPO. Many studies have reported high MDA in the serum, plasma and synovial fluid of RA patients [22,28,31]. MDA has an important role in pathogenesis of RA.…”

Section: Resultsmentioning

confidence: 99%

“…The study by Aryaein et al [54] showed that GR, vitamin E, Beta-carotene was lower and MDA was higher in the patient group than in controls. Kamanli et al [22] observed significantly lower GSH-Px, catalase, levels of GSH in plasma of RA patients. However higher GR activity have also been reported in RA [55].…”

Section: Mdamentioning

confidence: 97%

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Antioxidant Enzymes in Rheumatoid Arthritis

Kumar¹,

Prakash²,

Gupta³

et al. 2016

J Arthritis

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Joint destruction in rheumatoid arthritis (RA) is due to tissue injury in the area caused by inflammatory reactions, release of MMPs and free radicals produced by neutrophils and macrophages. The control of free radical production may have therapeutic roles thus the study was done to check the status of lipid peroxidation product malondialdehyde (MDA) and a few antioxidant enzymes in RA patients. 45 RA patients and 40 controls were selected. Controls were asymptomatic and RA patients were selected according to ACR criteria. RA patients had significantly high MDA, SOD and ALP and reduced activity of catalase and GR as compared to controls. SOD showed positive correlation with ALP. GR was positively related with MDA, SOD and ALP. The study shows that MDA is involved in the pathogenesis of RA. The system is trying to quench free radicals by high SOD activity. Higher production of H 2 O 2 or some other mechanism is responsible for inhibition of catalase and GR. However system is trying to reduce the damage by neutralizing superoxide anion. Therapeutic intervention of the oxidative stress may be considered for effective control of inflammation in RA patients.

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“…However our study is in line with [13,22] who have reported increased SOD levels in RA patients. Mazetti et al [13] have reported higher serum copper/Zn superoxide in patients with RA.…”

Section: Mdasupporting

confidence: 93%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Mdamentioning

confidence: 97%

See 3 more Smart Citations

Antioxidant Enzymes in Rheumatoid Arthritis

Kumar¹,

Prakash²,

Gupta³

et al. 2016

J Arthritis

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Induction of CCR2‐dependent macrophage accumulation by oxidized phospholipids in the air‐pouch model of inflammation

Kadl

Galkina

Leitinger

2009

Arthritis & Rheumatism

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Objective. Macrophages are key players in the pathogenesis of rheumatoid synovitis as well as in atherosclerosis. To determine whether atherogenic oxidized phospholipids potentially contribute to synovial inflammation and subsequent monocyte/macrophage recruitment, we examined the effects of oxidized 1-palmitoyl-2-arachidonoyl-sn-3-glycero-phosphorylcholine (OxPAPC) on chemokine expression and leukocyte recruitment in a facsimile synovium in vivo using the murine air-pouch model.Methods. Air pouches were raised by 2 injections of sterile air, and inflammation was induced by injecting either lipopolysaccharide (LPS) or OxPAPC into the pouch lumen. Inflammation was assessed by analysis of inflammatory gene expression using reverse transcription-polymerase chain reaction or immunohistochemical analysis, and leukocytes were quantified in the lavage fluid and in the pouch wall after staining with Giemsa or after enzymatic digestion followed by fluorescence-activated cell sorter analysis.Results. Application of OxPAPC resulted in selective recruitment of monocyte/macrophages into the airpouch wall, but not in the lumen. In contrast, LPS induced both monocyte and neutrophil accumulation in the pouch lumen as well as in the wall. LPS, but not OxPAPC, induced the expression of adhesion molecules E-selectin, P-selectin, intercellular adhesion molecule 1, and vascular cell adhesion molecule 1. OxPAPC increased the expression of the CCR2 ligands monocyte chemotactic protein 1 (MCP-1), MCP-3, and MCP-5, as well as RANTES and growth-related oncogene ␣ (GRO␣), while it down-regulated the expression of CCR2 on macrophages. Moreover, oxidized phospholipid-induced macrophage accumulation was abrogated in CCR2 ؊/؊ mice. Conclusion. These data demonstrate that oxidized phospholipids trigger a type of inflammatory response that leads to selective macrophage accumulation in vivo, a process relevant for the pathogenesis of chronic inflammatory rheumatic diseases.

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Markers of oxidative and nitrosative stress in systemic lupus erythematosus: Correlation with disease activity

Wang¹,

Pierangeli²,

Papalardo³

et al. 2010

Arthritis & Rheumatism

148

172

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Objective. Free radical-mediated reactions have been implicated as contributors in a number of autoimmune diseases, including systemic lupus erythematosus (SLE). However, the potential for oxidative/ nitrosative stress to elicit an autoimmune response or to contribute to disease pathogenesis, and thus be useful when determining a prognosis, remains largely unexplored in humans. This study was undertaken to investigate the status and contribution of oxidative/ nitrosative stress in patients with SLE.Methods. Sera from 72 SLE patients with varying levels of disease activity according to the SLE Disease Activity Index (SLEDAI) and 36 age-and sex-matched healthy controls were evaluated for serum levels of oxidative/nitrosative stress markers, including antibodies to malondialdehyde (anti-MDA) protein adducts and to 4-hydroxynonenal (anti-HNE) protein adducts, MDA/HNE protein adducts, superoxide dismutase (SOD), nitrotyrosine (NT), and inducible nitric oxide synthase (iNOS).Results. Serum analysis showed significantly higher levels of both anti-MDA/anti-HNE protein adduct antibodies and MDA/HNE protein adducts in SLE patients compared with healthy controls. Interestingly, not only was there an increased number of subjects positive for anti-MDA or anti-HNE antibodies, but also the levels of both of these antibodies were statistically significantly higher among SLE patients whose SLEDAI scores were >6 as compared with SLE patients with lower SLEDAI scores (SLEDAI score <6). In addition, a significant correlation was observed between the levels of anti-MDA or anti-HNE antibodies and the SLEDAI score (r ‫؍‬ 0.734 and r ‫؍‬ 0.647, respectively), suggesting a possible causal relationship between these antibodies and SLE. Furthermore, sera from SLE patients had lower levels of SOD and higher levels of iNOS and NT compared with healthy control sera.Conclusion. These findings support an association between oxidative/nitrosative stress and SLE. The stronger response observed in serum samples from patients with higher SLEDAI scores suggests that markers of oxidative/nitrosative stress may be useful in evaluating the progression of SLE and in elucidating the mechanisms of disease pathogenesis.

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Plasma lipid peroxidation and antioxidant levels in patients with rheumatoid arthritis

Cited by 158 publications

References 20 publications

Antioxidant Enzymes in Rheumatoid Arthritis

Antioxidant Enzymes in Rheumatoid Arthritis

Induction of CCR2‐dependent macrophage accumulation by oxidized phospholipids in the air‐pouch model of inflammation

Markers of oxidative and nitrosative stress in systemic lupus erythematosus: Correlation with disease activity

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