Plasma Levels of Interleukin 12 Family Cytokines and Their Relevant Cytokines in Adult Patients with Chronic Immune Thrombocytopenia before and after High-Dose Dexamethasome Treatment

Li, Qingsheng; Zhang, Linjie; Xia, Ruixiang; Zeng, Qingshu; Wang, Yongqing; Xia, Liang; Xia, Leiming; Yang, Mingzhen

doi:10.1159/000433472

Cited by 12 publications

(9 citation statements)

References 30 publications

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“…Knowing this discrepancy regarding the importance of IL‐12 in endometriosis, we measured IL‐12 serum levels in endometriosis patients and healthy controls; however, we were unable to detect this cytokine in the serum samples. According to previous studies, serum levels of IL‐12 p70 varied from 1.5 to 2000 pg/ml in the control and different pathological conditions (Biswas et al., 2020; Li et al., 2015); therefore, we used an ELISA kit whose detection range was 4–500 pg/ml. We suggest using ELISA kits with higher sensitivity (0.1 pg/ml) might help to detect IL‐12 in the serum samples of controls or patients suffering from endometriosis.…”

Section: Discussionmentioning

confidence: 99%

Association between gene polymorphisms of IL‐12, IL‐12 receptor and IL‐27 and organ involvement in Iranian endometriosis patients

Zare

Hesampour

Poordast

et al. 2022

Int J Immunogenetics

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Endometriosis is an inflammatory disease characterized by the presence of ectopic endometrial tissue, immune cell dysfunction and abnormal cytokine secretion. In addition to immunological factors, genetic variations that influence endometriosis severity and cytokine expression levels play important roles in the pathogenesis of this disease. Interleukin-12 (IL-12), specifically its p40 subunit encoded by IL-12B gene and the interleukin-12 receptor β1 (IL-12Rβ2) chain of its receptor, as well as interleukin-27 (IL-27) are important in the establishment of endometriosis. So, in this study, we measured IL-12 and IL-27 serum levels and investigated the possible links between IL-12B rs3212227, IL-12Rβ2 rs3790565 and IL-27 rs153109 polymorphisms and the risk of developing endometriosis in a group of Iranian women. In this case-control study, 162 endometriosis patients and 151 healthy women were included and tested for the aforementioned polymorphisms using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. The enzyme-linked immunosorbent assay (ELISA) method was also used to measure IL-12 and IL-27 serum levels. Although there was no statistically significant association between the genotypes and alleles of the studied polymorphisms and the development of endometriosis in general, the AA genotype of IL-12B rs3212227 showed a significant association with uterine endometriosis when compared to AC+CC genotypes (p = .04, CI = 0.270-0.988, OR = 0.517). Indeed, the AA genotype of the IL-12B rs3212227 single nucleotide polymorphism (SNP) may be linked with a lower risk of developing uterine endometriosis.There was no significant difference in IL-27 levels between the two studied groups (p = .49), and IL-12 levels were undetectable in both groups. In conclusion, the AA genotype of IL-12B rs3212227 might be associated with a decreased risk of uterine involvement in endometriosis patients.

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Section: Discussionmentioning

confidence: 99%

Association between gene polymorphisms of IL‐12, IL‐12 receptor and IL‐27 and organ involvement in Iranian endometriosis patients

Zare

Hesampour

Poordast

et al. 2022

Int J Immunogenetics

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“…An imbalance in Th1/Th2 cells and an increased proportion of Th17 cells were observed in patients with ITP, suggesting a role of T-cell dysfunction in ITP pathogenesis [14][15][16]. Several observational studies showed that HD-DXM treatment corrects the Th1-/Th2-chemokine imbalance [17], abnormal T-cell subsets [18], and serum cytokine expression imbalance in ITP [19,20]. Regulatory B-cells may play a role in ITP pathogenesis.…”

Section: Discussionmentioning

confidence: 99%

Very Early Increased Platelet Count within a Week after Initiation of High-Dose Dexamethasone Treatment Is Associated with Long-Term Response in Newly Diagnosed Immune Thrombocytopenia Patients

et al. 2021

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To evaluate the long-term efficacy of high-dose dexamethasone (HD-DXM) treatment for immune thrombocytopenia (ITP), we retrospectively analysed 36 newly diagnosed ITP patients treated with HD-DXM as a first-line treatment. An initial response was obtained in 23 (63.9%) patients, including 11 with a complete response (CR) and 12 with a partial response (PR). Six months after HD-DXM treatment, 26 of 33 (78.8%) evaluable patients achieved objective responses, including 18 CR and 8 PR. Among 13 patients without initial response, very early increased platelet count within a week (VEIP) was observed in 7 patients, 5 (71.4%) of whom achieved a response at 6 months. In 29 patients who had available platelet count within a week, patients showing VEIP revealed longer survival than those who did not (<i>p</i> = 0.026). HD-DXM was an effective treatment for newly diagnosed ITP patients. VEIP after HD-DXM treatment initiation was associated with a long-term objective response in newly diagnosed ITP patients.

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“… 60 After high-dose dexamethasone, the plasma levels of IL-12p70, IL-23, IL-27, IFN-γ and IL-17A were significantly decreased in patients with ITP compared with patients before high-dose dexamethasone treatment. 61 Conversely, the levels of IL-4, IL-10 and IL-35 in patients with ITP were considerably higher than those before treatment. 61 Similarly, intravenous immunoglobulin treatment corrects the peripheral deficiency of T reg cells, and increases IL-35 levels and platelet counts.…”

Section: Potential Relationship Between Il-35 and Itpmentioning

confidence: 90%

“… 61 Conversely, the levels of IL-4, IL-10 and IL-35 in patients with ITP were considerably higher than those before treatment. 61 Similarly, intravenous immunoglobulin treatment corrects the peripheral deficiency of T reg cells, and increases IL-35 levels and platelet counts. 62 These findings suggest that IL-35 levels might be used to evaluate the effectiveness of clinical treatment and predict disease severity.…”

Section: Potential Relationship Between Il-35 and Itpmentioning

confidence: 90%

Immunomodulatory cytokine interleukin-35 and immune thrombocytopaenia

Zhu

Shan

2020

J Int Med Res

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Considerable attention has been paid to interleukin (IL)-35 because of its immunosuppressive effects in a variety of autoimmune diseases. IL-35, a recently identified cytokine of the IL-12 family, is a negative regulatory factor secreted by IL-35-inducible regulatory T cells (iTr35 cells) and the recently reported regulatory B cells (Breg cells). Four biological effects of IL-35 have been discovered in vitro and in vivo: (i) suppression of T cell proliferation; (ii) conversion of naive T cells into iTr35 cells; (iii) downregulation of type 17 helper T (Th17) cells; and (iv) conversion of Breg cells into a Breg subset that produces IL-35 and IL-10. IL-35 plays an important role in a variety of autoimmune diseases, such as rheumatoid arthritis, allergic asthma and systemic lupus erythematosus. Primary immune thrombocytopaenia (ITP), which is characterized by isolated thrombocytopaenia and mild mucocutaneous to life-threatening bleeding, is an autoimmune disease with complex dysregulation of the immune system. Both antibody-mediated and/or T cell-mediated platelet destruction are key processes. In addition, impairment of T cells and cytokine imbalances have now been recognized to be important. This review summarizes the immunomodulatory effects of IL-35 and its role in the pathogenesis of ITP as mediated by T and B cells.

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Plasma Levels of Interleukin 12 Family Cytokines and Their Relevant Cytokines in Adult Patients with Chronic Immune Thrombocytopenia before and after High-Dose Dexamethasome Treatment

Cited by 12 publications

References 30 publications

Association between gene polymorphisms of IL‐12, IL‐12 receptor and IL‐27 and organ involvement in Iranian endometriosis patients

Association between gene polymorphisms of IL‐12, IL‐12 receptor and IL‐27 and organ involvement in Iranian endometriosis patients

Very Early Increased Platelet Count within a Week after Initiation of High-Dose Dexamethasone Treatment Is Associated with Long-Term Response in Newly Diagnosed Immune Thrombocytopenia Patients

Immunomodulatory cytokine interleukin-35 and immune thrombocytopaenia

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