Plasma L-Methionine Levels Following Intravenous Administration in Humanss

Harper, Harold A.; Kinsell, Laurance W.; Barton, Harry C.

doi:10.1126/science.106.2753.319

Cited by 13 publications

(5 citation statements)

References 3 publications

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“…Resolution of the peak in the tryptophan-ornithine position was not attempted here. Microbiological values for methionine (Harper, Kinsell & Barton, 1947), threonine, isoleucine, leucine and histidine (Hier & Bergeim,. 1946) seem all comparatively high (Table 3).…”

Section: Resultsmentioning

confidence: 99%

The excretion of amino acids by the human. A quantitative study with ion-exchange chromatography

Evered

1956

Biochemical Journal

135

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Section: Resultsmentioning

confidence: 99%

The excretion of amino acids by the human. A quantitative study with ion-exchange chromatography

Evered

1956

Biochemical Journal

135

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“…Apparent D-methionine concentrations were obtained by difference. All other analytical details were as described in previous publications from this laboratory (7,8). Standard chemical methods were used for the sulfur and amino acid determinations (9,10).…”

Section: Methodsmentioning

confidence: 99%

Studies in Methionine and Sulfur Metabolism. I. The Fate of Intravenously Administered Methionine, in Normal Individuals and in Patients With Liver Damage 12

Kinsell¹,

Harper²,

Barton³

et al. 1948

J. Clin. Invest.

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The cardinal role of the liver in protein and amino acid metabolism has been well established. Consequently, studies of amino acid metabolism occupy a natural and fundamental place in an investigation of metabolic aberrations in hepatic disease. In 1913, Van advantage since this serves as a "label" in the interpretation of the metabolic fate of the administered methionine.In the present study a standard dose of DLmethionine was administered intravenously. Observations were then made on the rate of disappearance of the amino acid from the plasma together with a comparison of the excretion of free methionine and sulfate sulfur before and after the substance was given. Since the racemic DLmethionine was used, it was of interest to follow the metabolism of both isomers. This was made possible by the separate analysis of samples by two micro-organisms, one utilizing only the Lisomer, the other, both D-and L-isomers. METHODSAll patients (veteran and naval personnel) received a standard high protein, high carbohydrate intake over a two-day period preceding the test, during which time all medication was discontinued. They received no food from 8:00 p.m. the preceding night until after the 180-minute blood and urine samples had been obtained. Methionine determinations were made on deproteinized filtrates of plasma obtained from heparinized blood. After removal of a blood sample for determinations of the fasting methionine level, 50 cc. of a 3 per cent DLmethionine solution were injected over a five-minute period. Blood samples were then drawn at 15, 30, 60, 120, and 180 minutes after the injection. Urine samples corresponding to a two-hour period prior to the administration of the test dose of methionine as well as to the subsequent three-hour period were collected and assayed for methionine and, in some cases, sulfate sulfur (both free and total), and total amino nitrogen. This latter constituent will be discussed in a later communication. In several instances, methionine excretion during various periods up to 48 hours after the test dose had been given was measured.Methionine was determined microbiologically using as test organisms Leuconostoc mesenteroides, P-60, for the assay of L-methionine and Lactobacillus fermenti, 36, for 677

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“…of blood. After centrifugation and separation of the plasma, this was, for the assay of the free methionine content, diluted I /3 and freed from protein by heat coagulation at pH 4.7 (Harper, Kinsell, and Barton, 1947). The protein-free filtrate was used for assay after adjustment of the pH to 6.8.…”

Section: Experimerttal Detailsmentioning

confidence: 99%

The Metabolism of Methionine and Choline in Normal and Pre‐eclamptic Pregnant Women

Sims

1951

BJOG

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Plasma L-Methionine Levels Following Intravenous Administration in Humanss

Cited by 13 publications

References 3 publications

The excretion of amino acids by the human. A quantitative study with ion-exchange chromatography

The excretion of amino acids by the human. A quantitative study with ion-exchange chromatography

Studies in Methionine and Sulfur Metabolism. I. The Fate of Intravenously Administered Methionine, in Normal Individuals and in Patients With Liver Damage 12

The Metabolism of Methionine and Choline in Normal and Pre‐eclamptic Pregnant Women

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