Plasma Kinetics of Th1, Th2 and Th17 Cytokines in Polymyositis Related to Chronic Graft-versus-Host Disease

Koh, Shiro; Koh, Hideo; Nakashima, Yasuhiro; Katayama, Takako; Sakabe, Manami; Okamura, Hiroshi; Yoshimura, Takuro; Nanno, Satoru; Nishimoto, Mitsutaka; Hayashi, Yoshiki; Nakane, Takahiko; Nakamae, Hirohisa; Ohsawa, Masahiko; Hino, Masayuki

doi:10.2169/internalmedicine.55.6206

Cited by 4 publications

(2 citation statements)

References 26 publications

(38 reference statements)

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“…Regarding CCL2, its plasma concentration seems to increase with a rise in alloreactive T cells in pulmonary and hepatic GVHD [19,20], but this effect was not observed in the intestine In silico protein-protein interaction network of up-regulated genes in patients with oGVHD and patients without GVHD. The nodes between genes represent the protein-protein interaction pairs from the STRING (Search Tool for the Retrieval of Interacting Genes/Proteins) database.…”

Section: Discussionmentioning

confidence: 99%

Gene expression profile of chronic oral graft-versus-host disease

et al. 2022

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Among the complications observed after allogeneic hematopoietic stem cell transplantation, graft-versus-host disease (GVHD) is the primary cause of post-transplant mortality. The oral cavity is the second most affected organ target in chronic GVHD. Tissue damage results from the upregulation of inflammatory mediators, which play a critical role in the immunopathogenesis of the disease. This case series observational study aims to evaluate the participation of cytokines, chemokines, transcription factors, and heat shock proteins in the pathogenesis of oral GVHD (oGVHD), describing the mRNA expression of 28 genes selected. Peripheral blood mononuclear cells were isolated from six participants with oGVHD and two without GVHD, and relative expression of transcripts with established roles as inflammatory mediators was determined in triplicate using the human RT2 Profiler™ PCR Array. The gene expression levels in the group with oGVHD were mainly up-regulated compared to those without GVHD. PBMC from oGVDH expressed consistently higher IFN-γ, TNF, IL-1β, CCL2, HSP60 (HSPD1) and HSP90 (HSP90B1). These results can provide a basis for developing new molecular diagnostics and targets therapies for the clinical management of oGVHD.

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Section: Discussionmentioning

confidence: 99%

Gene expression profile of chronic oral graft-versus-host disease

et al. 2022

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“…It has been reported that IL-17 is the most potent inflammatory mediator in autoimmune-mediated tissue damage and in tissue injury after transplantation (Chung et al 2012; Fujino et al 2003; Gigante et al 2011). In HSCT patients, IL-17 level increases in blood and at the tissue site involved in acute or cGvHD (Chen et al 2007; Dander et al 2009; Hill et al 2010; Koh et al 2016; Liu et al 2013; Ratajczak et al 2010). Our own study on IL-17 documented that Th17 cells are rather at a low level in the blood at the onset of aGvHD likely being marginalized in the affected tissues (Dlubek et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Diverse Activity of IL-17+ Cells in Chronic Skin and Mucosa Graft-Versus-Host Disease

Klimczak

Suchnicki²,

Sędzimirska³

et al. 2019

Arch. Immunol. Ther. Exp.

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Excessive inflammatory environment in a course of chronic graft-versus-host disease (cGvHD) is associated with T-cell trafficking into inflamed tissues. This study focused on the identification of IL-17-producing cells in the tissue biopsies of cGvHD patients. Forty-one biopsy specimens of cGvHD lesions of the skin ( n = 27), gastrointestinal tract ( n = 9) and oral mucosa ( n = 5), examined in 24 patients, were morphologically defined according to the NIH criteria and analyzed for the presence of cellular infiltrations including: IL-17 + , FOXP3 + and CCR6 + cells. IL-17 + cells were identified in 26/27 skin and in all gut and oral mucosa biopsies, being more frequent in mucosa lesions than in the skin (11/14 vs 14/26, respectively; NS: not significant). Double staining documented that CD138 + /IL-17 + cells were commonly seen in the gut than in the skin (5/8 vs 3/11, respectively; NS). In the skin, cells expressing trafficking receptor CCR6 + were more frequent than IL-17 + cells compared to the mucosa (23/26 vs 2/13, respectively; p < 0.0001). CCR6 was present on a majority of IL-17 + cells in all examined skin biopsies but only in 6 out of 11 digestive tract biopsies ( p = 0.0112). FOXP3 + cells were identified only in five patients (with mild lesions) at least in one biopsy. In this study group, results documented that local expansion of IL-17-producing cells in the digestive tract correlate with moderate and severe clinical symptoms of cGvHD, in contrast to the skin, where IL-17 + cells are rather scarcely present ( p = 0.0301) and the course of cGvHD is slowly progressing with final organ deterioration.

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