Plasma Isocitrate Dehydrogenase as a Marker of Centrilobular Hepatic Necrosis in Patients With Hyperthyroidism

Chung, Young Hwa; Jung, Sung Ae; Song, Byung Cheol; Chang, Woo Young; Kim, Jeong‐A; Song, Il Han; Kim, Jung Weon; Choi, Won Beom; Shong, Young Kee; Lee, Yung Sang; Suh, Dong Jin

doi:10.1097/00004836-200108000-00005

Cited by 15 publications

(7 citation statements)

References 18 publications

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“…in bronchoalveolar lavage fluid, as was demonstrated by Guo et al (2005). IDH was also found in the plasma (Ellis & Goldberg, 1971;Chung et al, 2001). These findings suggest extracellular metabolic functions of IDH (Mitchell et al, 2008).…”

Section: Discussionsupporting

confidence: 57%

Identification of specific interaction of juvenile hormone binding protein with isocitrate dehydrogenase.

Zalewska

Ożyhar²,

Kochman³

2011

Acta Biochim Pol

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Juvenile hormone (JH) is essential for multiple physiological processes: it controls larval development, metamorphosis and adult reproduction. In insect hemolymph more than 99 % of JH is bound to juvenile hormone binding protein (JHBP), which protects JH from degradation by nonspecific hydrolases and serves as a carrier to supply the hormone to the target tissues. In Galleria mellonella hemolymph, JHBP is found in a complex with lipid-binding high molecular weight proteins (HMWP) and this interaction is enhanced in the presence of JH. In this report, we present studies on the interaction of JHBP with low molecular weight proteins (LMWP) in the hemolymph. Using ligand blotting we found that JHBP interacts with a protein of about 44 kDa. To identify the protein that preferentially binds JHBP, a LMWP fraction was applied to a Sepharose-bound JHBP and, after washing, the column was eluted with free JHBP acting as a specific competitor or with carbonic anhydrase as a negative control. The eluted proteins were separated by SDS/PAGE and analyzed by mass spectrometry. Isocitrate dehydrogenase was identified as a component of the supramolecular complex of JHBP with hemolymph proteins.

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Section: Discussionsupporting

confidence: 57%

Identification of specific interaction of juvenile hormone binding protein with isocitrate dehydrogenase.

Zalewska

Ożyhar²,

Kochman³

2011

Acta Biochim Pol

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“…The frequency of jaundice varies from 5.3% in uncomplicated hyperthyroid patients to 79% if hyperthyroidism‐induced congestive heart failure is present 14. Chung and associates reported that the plasma isocitrate dehydrogenase level or the isocitrate dehydrogenase/alanine aminotransferase ratio is useful for distinguishing centrilobular necrosis from periportal necrosis and for monitoring the degree of hepatic necrosis in patients with hyperthyroidism 15. Impaired hepatic metabolism may further exacerbate the hyperthyroid state.…”

Section: Discussionmentioning

confidence: 99%

Thyroid storm in a patient with fulminant hepatic failure

et al. 2010

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This manuscript describes a 28-year-old patient with a history of Graves' disease who was transferred to Tulane University Hospital with fulminant hepatic failure. He reported associated nausea, vomiting, anorexia, as well as jaundice and abdominal discomfort for a period of 3 weeks prior to his admission. His thyroid function tests on admission were TSH, 0.013 μU/mL; T3, 94.9 μU/mL; T4, 9.37 μU/mL; Free T4, >6 μU/mL. His liver function tests were characteristic of hepatic failure. The patient underwent an emergent liver transplant. His surgery was complicated by heart failure and acute respiratory distress syndrome. Given the patients clinical presentation and laboratory results, a diagnosis of thyroid storm was made and a decision was made to proceed with an emergent thyroidectomy. The posttransplant multiorgan dysfunction was rapidly reversed by prompt thyroidectomy and decisive management. The patient was discharged from the hospital with normal thyroid and liver function tests.

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“…Although elevations in serum ICDH are relatively specific for liver disorders, increased levels have also been reported in disseminated malignant disease without detectable hepatic involvement. Like glutamate dehydrogenase, ICDH is predominantly found in hepatocytes in the centrilobular zone [33]. The measurement of ICDH does not yet offer any diagnostic advantage over the measurement of aminotransferases.…”

Section: Isocitrate Dehydrogenasementioning

confidence: 99%

Laboratory Tests

Herlong

Mitchell

2011

Schiff's Diseases of the Liver

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Key conceptsr Biochemical tests can be used to detect acute injury to the liver and chronic diseases of the liver before development of symptoms.r Elevations in serum levels of aminotransferases (aspartate aminotransferase and alanine aminotransferase) reflect hepatocellular injury and/or permeability of the liver cell membrane, but do not correlate with the extent of fibrosis or measures of metabolic functions of the liver.r An elevation of serum alkaline phosphatase occurs in response to intrahepatic cholestasis, extrahepatic obstruction of bile ducts, or infiltration of the liver with granulomatous inflammation, amyloidosis, or venous congestion.r The rate-limiting step in metabolism and disposition of bilirubin is the transport of conjugated bilirubin from the hepatocyte into the bile.An elevation in the conjugated (direct-reacting) fraction of bilirubin is evidence of intrahepatic cholestasis or extrahepatic obstruction of the bile ducts.r Serum levels of albumin may be low in patients with acute or chronic liver disease, but very often drop in response to the "acute phase response," making it difficult to evaluate hepatic metabolic function in clinical situations such as infection.r Although single laboratory tests are generally not useful in evaluating the severity of acute or chronic liver diseases, the use of mathematical equations combining tests may be effective as surrogate markers for the extent of fibrosis, steatosis, and inflammation as well as to predict the short-term prognosis in patients with liver disease.The use of laboratory tests is an important component of the assessment of patients with liver disease. With increasing frequency, elevations in the hepatic enzymes detected on routine screening tests provide the first evidence of liver disease in otherwise asymptomatic patients. In those patients with previously recognized hepatic dysfunction, laboratory tests can help establish the etiology of liver disease and provide valuable prognostic information. Rarely does a single test provide sufficient information to establish a diagnosis or assess severity of liver disease. A combination of tests such as serum bilirubin, albumin, aminotransferases, and alkaline phosphatase is sometimes referred to "liver function tests" or a "liver panel." These tests, in combination with the prothrombin time can provide an initial characterization of the etiology and severity of liver disease. However, one should recognize that the aminotransferases and alkaline phosphatase are tests that reflect hepatic injury not function. Traditionally liver diseases have been characterized as primarily hepatocellular or cholestatic based on the predominance of elevated aminotransferases or alkaline phosphatase. While an individual laboratory test may not provide a specific diagnosis, the pattern of abnormalities can suggest a general category of hepatic dysfunction. Although this distinction helps direct the initial evaluation there is often considerable overlap so that aggregating the diseases is less useful, as in the case...

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Plasma Isocitrate Dehydrogenase as a Marker of Centrilobular Hepatic Necrosis in Patients With Hyperthyroidism

Cited by 15 publications

References 18 publications

Identification of specific interaction of juvenile hormone binding protein with isocitrate dehydrogenase.

Identification of specific interaction of juvenile hormone binding protein with isocitrate dehydrogenase.

Thyroid storm in a patient with fulminant hepatic failure

Laboratory Tests

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