Plasma iron and transferrin iron-binding capacity evaluated by colorimetric and immunoprecipitation methods.

Huebers, Helmut A.; Eng, Marlene; Josephson, Betty; Ekpoom, Naruemol; Rettmer, Rebecca L.; Labbé, Robert F.; Pootrakul, Pensri; Finch, C. A.

doi:10.1093/clinchem/33.2.273

Cited by 48 publications

(20 citation statements)

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“…For gonococci, abundant Hb is probably available during menses. To achieve minimum free iron concentrations, serum transferrin is only 30% saturated with iron, presenting would-be scavengers with a greater challenge: to bind selectively only iron bound transferrin 52 . TbpB overcomes this obstacle by binding specifically with high affinity to transferrin with iron-occupied C-lobe.…”

Section: Discussionmentioning

confidence: 99%

Structural analysis of haemoglobin binding by HpuA from the Neisseriaceae family

Wong

Gupta

et al. 2015

Nat Commun

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The Neisseriaceae family of bacteria causes a range of diseases including meningitis, septicaemia, gonorrhoea and endocarditis, and extracts haem from haemoglobin as an important iron source within the iron-limited environment of its human host. Herein we report crystal structures of apo- and haemoglobin-bound HpuA, an essential component of this haem import system. The interface involves long loops on the bacterial receptor that present hydrophobic side chains for packing against the surface of haemoglobin. Interestingly, our structural and biochemical analyses of Kingella denitrificans and Neisseria gonorrhoeae HpuA mutants, although validating the interactions observed in the crystal structure, show how Neisseriaceae have the fascinating ability to diversify functional sequences and yet retain the haemoglobin binding function. Our results present the first description of HpuA's role in direct binding of haemoglobin.

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Section: Discussionmentioning

confidence: 99%

Structural analysis of haemoglobin binding by HpuA from the Neisseriaceae family

Wong

Gupta

et al. 2015

Nat Commun

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“…The Hb concentration and Hct in the whole blood were determined by an automatic hematology analyzer (ABX Pentra DF 120; HORIBA Medical Inc., Montpellier, France; Southern and Baker, 1982;Aoyagi and Baker, 1995). The PI and TIBC were measured by using the colorimetric method (Bothwell et al, 1971;International Committee for Standardization in Haematology, 1978;Huebers et al, 1987). Transferrin saturation (Ts) in the plasma was calculated according to an equation: TS (%) = (PI/TIBC) × 100% (Huebers et al, 1987;Ma, 2012).…”

Section: Sample Analysesmentioning

confidence: 99%

“…The PI and TIBC were measured by using the colorimetric method (Bothwell et al, 1971;International Committee for Standardization in Haematology, 1978;Huebers et al, 1987). Transferrin saturation (Ts) in the plasma was calculated according to an equation: TS (%) = (PI/TIBC) × 100% (Huebers et al, 1987;Ma, 2012).…”

Section: Sample Analysesmentioning

confidence: 99%

The chemical characteristics of organic iron sources and their relative bioavailabilities for broilers fed a conventional corn–soybean meal diet1

Zhang

Lü

Luo

2016

Journal of Animal Science

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Twenty-four organic Fe sources were evaluated by polarographic analysis and via solubility in buffers (pH 5 and 2) and deionized water. Organic Fe sources included 6 Fe-Met complexes (Fe-Met), 10 Fe-Gly complexes, 1 Fe-Lys complex, 4 Fe proteinates, and 3 Fe-AA complexes (Fe-AA). Sources varied considerably in chemical characteristics. Chelation strengths (quotient of formation [Q] values) ranged from weak (Q = 1.08) to extremely strong strength (Q = 8,590). A total of 1,170 1-d-old Arbor Acres male broilers were randomly allotted to 6 replicate cages (15 chicks/cage) for each of 13 treatments in a completely randomized design involving a 4 × 3 factorial arrangement of treatments (4 Fe sources × 3 added Fe levels) plus a control with no added Fe. Dietary treatments included a corn-soybean meal basal diet (control; 55.8 mg Fe/kg) and the basal diet supplemented with 20, 40, or 60 mg Fe/kg as iron sulfate (FeSO∙7HO); an Fe-Met with weak chelation strength (Fe-Met W; Q = 1.37; 14.7% Fe); an iron proteinate with moderate chelation strength (Fe-Prot M; Q = 43.6; 14.2% Fe); or an iron proteinate with extremely strong chelation strength (Fe-Prot ES; Q = 8,590; 10.2% Fe). The growth performance, Fe concentrations, hematological indices, and activities and gene expressions of 2 Fe-containing enzymes in tissues of broilers at 7, 14, and 21 d of age were determined in the present study. Transferrin saturation in plasma on 14 d; bone Fe on d 7 and 14; liver Fe on d 7, 14, and 21; kidney Fe on d 14; succinate dehydrogenase activities in the liver on d 21 and in the kidney on d 7 and 21; mRNA levels in the kidney and heart on d 14; and mRNA levels in the liver and kidney on d 21 linearly increased ( < 0.05) as added Fe levels increased. However, differences in bioavailabilities among Fe sources were detected ( < 0.05) only for the mRNA levels in the liver and kidney on d 21. Based on slope ratios from the multiple linear regression of mRNA level in the liver or kidney of broilers on d 21 on daily dietary analyzed Fe intake, the bioavailabilities of Fe-Met W, Fe-Prot M, and Fe-Prot ES relative to iron sulfate (100%) were 129 ( = 0.18), 164 ( < 0.003), and 174% ( < 0.001) or 102 ( = 0.95), 143 ( = 0.09), and 174% ( < 0.004), respectively. These results indicated that the relative bioavailabilities of organic Fe sources were closely related to their Q values and organic Fe sources with greater Q values showed higher Fe bioavailabilities.

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“…In healthy individuals, approximately one-third of the iron binding sites are occupied (TSat 33%). 32 When the total ironbinding capacity of transferrin is exceeded, non-transferrin bound iron (NTBI) is produced. NTBI is virtually undetectable until TSat reaches 80%.…”

Section: Transferrin Saturationmentioning

confidence: 99%

Markers of iron status in chronic kidney disease

Gawęda

2017

Hemodialysis International

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Anemia is one of the main comorbidities related to Chronic Kidney Disease (CKD). Until the advent of Erythropoiesis Stimulating Agents (ESA), endogenous erythropoietin deficiency has been thought to be the main culprit of anemia in CKD patients. The use of ESA’s has shed new light on the physiology of CKD anemia, where iron homeostasis plays an increasingly important role. Disorders of iron homeostasis occurring in CKD turn the anemia management in those patients into a complex multifactorial therapeutic task, where ESA and Iron dose must be properly balanced to achieve the desired outcome without exposing the patients to the risk of serious adverse events. This review covers diagnostic markers traditionally used for quantifying iron status in CKD patients, such as serum ferritin and transferrin saturation, new ones, such as reticulocyte hemoglobin content and percent hypochromic red cells, as well as experimental ones, such as hepcidin and soluble transferrin receptor. Each marker is presented in terms of their diagnostic performance, followed by biological and analytical variability data. Advantages and disadvantages of each marker are briefly discussed. Although serum ferritin and transferrin saturation are easily available, they exhibit large biological variability and require caution when used for diagnosing iron status in CKD patients. Reticulocyte hemoglobin content and the percentage of hypochromic red cells are more powerful, but their widespread use is hampered by the issue of sample stability in storage. Soluble transferrin receptor and hepcidin show promise, but require further investigation as well as the development of standardized, low-cost assay platforms.

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Plasma iron and transferrin iron-binding capacity evaluated by colorimetric and immunoprecipitation methods.

Cited by 48 publications

References 0 publications

Structural analysis of haemoglobin binding by HpuA from the Neisseriaceae family

Structural analysis of haemoglobin binding by HpuA from the Neisseriaceae family

The chemical characteristics of organic iron sources and their relative bioavailabilities for broilers fed a conventional corn–soybean meal diet1

Markers of iron status in chronic kidney disease

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