Plasma-induced nanostructured metallic silver surfaces: study of bacteriophobic effect to avoid bacterial adhesion on medical devices

García‐Bonillo, Cristina; Texidó, Robert; Gilabert‐Porres, Joan; Borrós, Salvador

doi:10.1016/j.heliyon.2022.e10842

Cited by 5 publications

(6 citation statements)

References 54 publications

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“…The study reported on the adaptative nature of the bacterial strains and gave small variability in the colonies and individual cells were hardly noticeable, furthermore, these changes were associated with the changes in the climatic conditions. In addition, García-Bonillo et al ., [27] and Tsai et al ., [28] found bacterial morphological similarities and related it to the increased immobilization of bacterial colony film upon the chain in conditions of growth.…”

Section: Fig 1 Phylogenetic Analysis Of Nucleotide Sequences Of Soft ...mentioning

confidence: 94%

Isolation and Identification of Bacterial Soft-Rot-Causing Isolates from Cassava, Homa-Bay County, Kenya

Otieno D.,

Muraya M. M.,

Mungiria J. N.

2023

IJPR

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Cassava bacterial soft rot is of great importance to economically valuable crop varieties. Commonly, bacterial soft rot strains cause destructive vegetable and potato diseases worldwide. The strain act similar to other species of the family Enterobacteriaceae that cause soft rot in plants. However, the disease has spread in a wide range of environment with its prevalence and causative strains less known to most agroecological zones of Kenya. Generally, aim of this study was to isolate, characterize and identify bacterial soft rot causative strains in cassava, Homa-Bay County. At the Sub County level, 104 farms were identified through a simple random method and symptomatic cassava plants sampled along laid transect in each farm. Rotten cassava tubers were then randomly sampled, packed and taken to the laboratory for pathogen identification. The bacterial strains were isolated in Nutrient Agar at 37˚c for 24 hours to obtain pure cultures. Identification was done using morphological, biochemical and 16S rRNA analysis method. The study identified: two Pectobacterium carotovorum, three Bacillus species: Bacillus amyloliquefaciens, Bacillus subtilis, and Bacillus tropicus, and three Achromobacter species: Achromobacter marplatensis, Verticiella sediminum, and Achromobacter xylosoxidans, pathogenic strains causing cassava bacterial soft rot. In this study, characterization and identification of cassava bacterial soft rot in Homa Bay County was done for the first time. The results can not only be used in the characterization enzymes produced by cassava bacterial soft rot strains but also in the development of effective control strategy.

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Section: Fig 1 Phylogenetic Analysis Of Nucleotide Sequences Of Soft ...mentioning

confidence: 94%

Isolation and Identification of Bacterial Soft-Rot-Causing Isolates from Cassava, Homa-Bay County, Kenya

Otieno D.,

Muraya M. M.,

Mungiria J. N.

2023

IJPR

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“…pp-PFM Thin Film Deposition: The plasma polymerization of PFM was carried out as described in the previous bibliography of our group. [49,35] Substrates were placed on an aluminum plate in the center of the reactor. Before the introduction of the substrate, the chamber was cleaned under O 2 /Ar (1:1) plasma for 1 h at a power of 150 W. The continuous radio frequency power was fixed at 15 W, and pulsed plasma polymerization duty cycle (DC) of 10/20 [DC = T on /(T on + T off )] was carried out for 3 min.…”

Section: Methodsmentioning

confidence: 99%

“…This system has been widely studied in our group as silver reservoirs to provide bacteriophobic/antibacterial properties to elastomeric materials. [35,36] However, under specific conditions of Tollens' reaction, silver nucleation produces a homogeneous surface of silver microplates that can be easily separated under stress, revealing a polymer-silver interface directly attached to the substrate that allows the electrical conductivity under high strain levels (near 100% strain). These structural changes produce variations in conductivity that, with the appropriate design, allow it to work as a highly sensitive strain sensor, reaching GF values up to 900 000.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive Silver‐Microlayer Thin Film on a pp‐PFM‐Modified PDMS Strain Sensor as a Versatile Tool for Stretchable Electronics Applications

Texidó

Nieva‐Esteve

Gilabert‐Porres³

et al. 2022

Adv Elect Materials

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Strain sensors for wearable electronic devices have received attention due to their potential application in medicine for physiological monitoring or as part of advanced prosthetics. However, low sensitivity values as well as complex fabrication procedures remain significant challenges limiting the applicability. This work presents the fabrication of a strain sensor based on the separation of silver microplates immobilized on a stretchable substrate. The deposition of the microplates is achieved through the exposition of a glucosamine‐functionalized surface to the Tollens’ reagent, being a versatile fabrication methodology able to be implemented in a wide range of substrates. The obtained sensors present a high stretchability (>100%), high conductivity (105 S m–1), good linearity (R2 > 0.98 under 30% strain), good hysteresis properties, and high sensitivity (up to GF = 900 000). Hence, the sensors allow the measurement of very small deformations even in dynamic range, where it presents a stable linear response for the quantification of cyclic deformations of 0.02% strain. Moreover, the applicability of these sensors has been studied in motion‐sensing devices and in a pressure sensor revealing that this technology may expand the potential applications of wearable electronic devices.

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“…This procedure has been previously reported by our group as a straightforward manner to immobilize biomolecules on surfaces for biomedical applications. 30,32,46 pp-PFM polymers can immobilize amine-containing biomolecules, thanks to the high electrophilic character of the perfluorinated ester function that assists a rapid amide formation (see Figure S31, from SI).…”

Section: Acsmentioning

confidence: 99%

“…The procedure of plasma polymerizations for those surface modifications was carried out as described in previous papers by our group. 31,32 Immobilization of Reducing Saccharides. Once the pp-PFM film was grafted over the surfaces, the samples were incubated in the presence of glucosamine 1 M at pH = 8 to perform the immobilization of this reducing sugar on pp-PFM for functionalizing surfaces (Figure S31).…”

Section: ■ Introductionmentioning

confidence: 99%

Designing Polyelectrolyte Microneedles Based on Borylated Poly(β-aminoester) Polymers To Enhance Transdermal pH-Controlled Delivery of Nucleic Acids

González-Sáenz,

Cosialls,

Texidó

et al. 2024

ACS Appl. Polym. Mater.

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The use of transdermal delivery for nucleic acid administration is an interesting approach to overcoming limitations of systemic administration routes, such as first-pass effects, the painful needle injection, or their poor biodistribution. Thus, the use of a microneedle-based patch could represent a turning point for nucleic acid delivery, thanks to the possibility of self-administration of the actives in a painless and easy procedure. However, the design of transdermal systems with a higher degree of precision release is a clear need that has not been fully resolved. Committed to tackling this challenge, we present here a microneedle patch that involves a smart delivery system supported by the well-established ability of boronic acid to interact with carbohydrates in a pH-dependent manner. This system builds up a multilayer structure over a solid microneedle platform whose surface has been modified to immobilize glucosamine units that are able to interact with an oligopeptide-end terminated poly(β-aminoester) that presents a 4-carboxy-3-fluorophenylboronic acid (Bor-pBAE). Thus, sequential layers of the Bor-pBAE and plasmid DNA have been assembled, thanks to the ability of the polymer to interact with the nucleic acid at a basic pH and then gradually release the plasmid under two different conditions of pH (the physiological pH = 7.4 and the acidic pH = 5.1). We set up the design and implementation of this first proof of concept while demonstrating microneedles' safety and functionality. Additionally, we have shown the efficacy of the construct to express the encoded genes in model cell lines. In conclusion, we have established the basis to confirm that this generation of borylated poly(β-aminoesters) holds great promise as a transdermal local nucleic acid delivery system.

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Plasma-induced nanostructured metallic silver surfaces: study of bacteriophobic effect to avoid bacterial adhesion on medical devices

Cited by 5 publications

References 54 publications

Isolation and Identification of Bacterial Soft-Rot-Causing Isolates from Cassava, Homa-Bay County, Kenya

Isolation and Identification of Bacterial Soft-Rot-Causing Isolates from Cassava, Homa-Bay County, Kenya

Highly Sensitive Silver‐Microlayer Thin Film on a pp‐PFM‐Modified PDMS Strain Sensor as a Versatile Tool for Stretchable Electronics Applications

Designing Polyelectrolyte Microneedles Based on Borylated Poly(β-aminoester) Polymers To Enhance Transdermal pH-Controlled Delivery of Nucleic Acids

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