Plasma-induced graft polymerization of acrylic acid onto poly(ethylene terephthalate) films: characterization and human smooth muscle cell growth on grafted films

Gupta, Bhuvanesh; Plummer, C. J. G.; Bisson, Isabelle; Frey, Peter; Hilborn, Jöns

doi:10.1016/s0142-9612(01)00195-8

Cited by 306 publications

(231 citation statements)

References 26 publications

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“…Similar finding was reported on AA-grafted polyurethane [42]. Quantitatively, based on the gravimetric data, the average graft density of PAA, assuming uniform distribution, is estimated to be «185 |jigcm -2 , which is higher than the average values attained in similar works devoted to surface grafting of PAA [41,43,44]. Interestingly, the estimated graft density is on the order of the surface absorbed water density of sample 3 assessed in the former section.…”

Section: Surface Morphology Analysissupporting

confidence: 82%

“…Some of the radicals may also be present below the top layer and initiate the grafting reaction. This brings about an apparently swelled grafted top layer following the diffusion of monomers and participation in chain propagation [41]. Indeed, the roughest morphology is observed for sample 3 compared to the others showing a brush-like pattern which forms an active support for subsequent modifications.…”

Section: Surface Morphology Analysismentioning

confidence: 97%

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An in vitro bacterial adhesion assessment of surface-modified medical-grade PVC

Asadinezhad

Novák

Lehocký

et al. 2010

Colloids and Surfaces B: Biointerfaces

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A B S T R A C TMedical-grade polyvinyl chloride was surface modified by a multistep physicochemical approach to improve bacterial adhesion prevention properties. This was fulfilled via surface activation by diffuse coplanar surface barrier discharge plasma followed by radical graft copolymerization of acrylic acid through surfaceinitiated pathway to render a structured high density brush. Three known antibacterial agents, bronopol, benzalkonium chloride, and chlorhexidine, were then individually coated onto functionalized surface to induce biological properties. Various modern surface probe techniques were employed to explore the effects of the modification steps. In vitro bacterial adhesion and biofilm formation assay was performed. Escherichia coli strain was found to be more susceptible to modifications rather than Staphylococcus aureus as up to 85% reduction in adherence degree of the former was observed upon treating with above antibacterial agents, while only chlorhexidine could retard the adhesion of the latter by 50%. Also, plasma treated and graft copolymerized samples were remarkably effective to diminish the adherence of E. coli.

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Section: Surface Morphology Analysissupporting

confidence: 82%

Section: Surface Morphology Analysismentioning

confidence: 97%

An in vitro bacterial adhesion assessment of surface-modified medical-grade PVC

Asadinezhad

Novák

Lehocký

et al. 2010

Colloids and Surfaces B: Biointerfaces

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“…One approach to surface modification is graft polymerization with an active monomer such as acrylic acid. 10 Poly(acrylic acid) promotes the adsorption of collagen and serum proteins, allowing better cell adhesion. However, excess of poly(acrylic acid) graft was reported to hinder cell growth.…”

Section: Introductionmentioning

confidence: 99%

Protein adsorption onto polyester surfaces: Is there a need for surface activation?

Atthoff¹,

Hilborn²

2006

J Biomed Mater Res

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Surface hydrolysis of polyester scaffolds is a convenient technique suggested to promote protein adsorption for improving cell attachment. We have, therefore, investigated the effect of hydrolysis of polyester surfaces for protein adsorption to clarify the conditions needed. Three polyesters, poly(ethylene terephthalate) (PET), poly(lactic acid) (PLA), and poly(glycolic acid) (PGA), were selected. Adsorption was investigated by X-ray photoelectron spectroscopy (XPS), scanning electron microscopy (SEM), and quartz crystal microbalance (QCM). Hydrolyzed PET adsorbed significantly more proteins than nonhydrolyzed. Degradable polymers adsorbed at higher rates when the polymers were hydrolyzed prior to adsorption, but the same amount as nonhydrolyzed, suggesting spontaneous hydrolysis during the adsorption. XPS shows that hydrolysis prior to absorption for PET results in a surface nitrogen composition of ϳ14%, similar to pure protein (16%). Nonhydrolyzed PET surfaces showed only ϳ7% nitrogen, indicating protein layers thinner than ϳ10 nm. Adsorption to PLA and PGA shows nitrogen contents of 14 -15% in both cases. SEM revealed striking differences in morphology of the protein coating. Hydrolyzed or spontaneously hydrolyzable surfaces display a pronounced fibrous structure while nonhydrolyzed surfaces give smooth structures. In combination, the results show that surface hydrolysis increase adsorption rate, but not the amount of proteins on polyesters that degrades in vivo. Surface treatment of nondegradable polyester increases the total amount of proteins and induces the formation of fibrous protein structures. Post hydrolysis treatment by acetic acid, replacing the counter-ion to a proton, further enhances protein attachment. Finally, cell attachment experiments verifies that protein adsorption increase the cell attachment to polyester surfaces.

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“…In recent years, artificial ECMs gained special interest due to their excellent mechanical properties, processability and low cost [17]. It is expected that synthetic ECMs can mimic many functions of the natural ECM including cellular 3D architecture, mechanical integrity to the new tissue and the space for the diffusion of nutrients and metabolites [18].…”

Section: Artificial Extracellular Matricesmentioning

confidence: 99%

Artificial extracellular matrix for embryonic stem cell cultures: a new frontier of nanobiomaterials

Haque

Nagaoka

Hexig

et al. 2010

Science and Technology of Advanced Materials

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Nanobiomaterials can play a central role in regenerative medicine and tissue engineering by facilitating cellular behavior and function, such as those where extracellular matrices (ECMs) direct embryonic stem (ES) cell morphogenesis, proliferation, differentiation and apoptosis. However, controlling ES cell proliferation and differentiation using matrices from natural sources is still challenging due to complex and heterogeneous culture conditions. Moreover, the systemic investigation of the regulation of self-renewal and differentiation to lineage specific cells depends on the use of defined and stress-free culture conditions. Both goals can be achieved by the development of biomaterial design targeting ECM or growth factors for ES cell culture. This targeted application will benefit from expansion of ES cells for transplantation, as well as the production of a specific differentiated cell type either by controlling the differentiation in a very specific pathway or by elimination of undesirable cell types.

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Plasma-induced graft polymerization of acrylic acid onto poly(ethylene terephthalate) films: characterization and human smooth muscle cell growth on grafted films

Cited by 306 publications

References 26 publications

An in vitro bacterial adhesion assessment of surface-modified medical-grade PVC

An in vitro bacterial adhesion assessment of surface-modified medical-grade PVC

Protein adsorption onto polyester surfaces: Is there a need for surface activation?

Artificial extracellular matrix for embryonic stem cell cultures: a new frontier of nanobiomaterials

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