Plasma glucose kinetics and tissue uptake in brown trout in vivo: effect of an intravascular glucose load

Blasco, Josefina; Fernández-Borràs, Jaume; Marimón, Irene; Requena, Antonio Hidalgo

doi:10.1007/bf00387514

Cited by 86 publications

(91 citation statements)

References 20 publications

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“…For example, in seabass the activities of hepatic gluconeogenic enzymes were not suppressed by chronic high-CHO feeding thereby contributing to postprandial hyperglycemia (Enes et al, 2006(Enes et al, , 2008b. Meanwhile, the transition from feeding to fasting is characterized by a steep drop in plasma glucose concentrations (Gutiérrez et al, 1991;Echevarría et al, 1997;Pérez-Jiménez et al, 2007;Viegas et al, 2011;Pérez-Jiménez et al, 2012) among other tissue-dependent effects (Blasco et al, 2001). Other fish species can tolerate extended periods of starvation without apparently suffering from hypoglycemia like Brycon cephalus (Figueiredo-Garutti et al, 2002), gilthead seabream Sparus aurata (Sangiao-Alvarellos et al, 2005;Polakof et al, 2006) or Senegalese sole Solea senegalensis (Costas et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Effects of food-deprivation and refeeding on the regulation and sources of blood glucose appearance in European seabass (Dicentrarchus labrax L.)

Viegas

Rito

González

et al. 2013

Comparative Biochemistry and Physiology Part A: Molecular &

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H 2 O Gluconeogenesis Glycogenolysis Glucose 6-phosphatase Glucokinase NMR Sources of blood glucose in European seabass (initial weight 218.0 ± 43.0 g; mean ± S.D., n = 18) were quantified by supplementing seawater with deuterated water (5%-2 H 2 O) for 72 h and analyzing blood glucose 2 H-enrichments by 2 H NMR. Three different nutritional states were studied: continuously fed, 21-day of fast and 21-day fast followed by 3 days of refeeding. Plasma glucose levels (mM) were 10.7 ± 6.3 (fed), 4.8 ± 1.2 (fasted), and 9.3 ± 1.4 (refed) (means ± S.D., n = 6), showing poor glycemic control. For all conditions, 2 H-enrichment of glucose position 5 was equivalent to that of position 2 indicating that blood glucose appearance from endogenous glucose 6-phosphate (G6P) was derived by gluconeogenesis. G6P-derived glucose accounted for 65 ± 7% and 44 ± 10% of blood glucose appearance in fed and refed fish, respectively, with the unlabeled fraction assumed to be derived from dietary carbohydrate (35 ± 7% and 56 ± 10%, respectively). For 21-day fasted fish, blood glucose appearance also had significant contributions from unlabeled glucose (52 ± 16%) despite the unavailability of dietary carbohydrates. To assess the role of hepatic enzymes in glycemic control, activity and mRNA levels of hepatic glucokinase (GK) and glucose 6-phosphatase (G6Pase) were assessed. Both G6Pase activity and expression declined with fasting indicating the absence of a classical counter-regulatory stimulation of hepatic glucose production in response to declining glucose levels. GK activities were basal during fed and fasted conditions, but were strongly stimulated by refeeding. Overall, hepatic G6Pase and GK showed limited capacity in regulating glucose levels between feeding and fasting states.

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Section: Introductionmentioning

confidence: 99%

Effects of food-deprivation and refeeding on the regulation and sources of blood glucose appearance in European seabass (Dicentrarchus labrax L.)

Viegas

Rito

González

et al. 2013

Comparative Biochemistry and Physiology Part A: Molecular &

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“…Recently, insulin has been shown to directly stimulate the uptake of glucose and amino acids by trout muscle cells in culture (9) and IGF-I shares the same effects, supporting the notion that insulin and IGF-I may directly regulate carbohydrate and protein metabolism in trout skeletal muscle. In brown trout, skeletal muscle (red and white) accounts for approximately half of the total glucose uptake by tissues despite having the lowest glucose uptake rates and is the only tissue that increases its glucose uptake rate in response to a glucose load (5). Therefore, skeletal muscle can be considered a major contributor of glucose disposal from the blood in fish, and its ability to regulate its glucose uptake rate suggests the possible involvement of a regulatable facilitative glucose transport system.…”

mentioning

confidence: 99%

Expression of rainbow trout glucose transporters GLUT1 and GLUT4 during in vitro muscle cell differentiation and regulation by insulin and IGF-I

Dı́az

Vraskou²,

Gutiérrez³

et al. 2009

American Journal of Physiology-Regulatory, Integrative and Comp

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“…(ATP) (Lucas, 1996). , , 54-180 mg/100 mL (West et al, 1994;Blasco et al, 2001) rock perch (Scorpaena porcus L.) (Silkin and Silkina, 2005) (Paralichthys olivaceus) (Hur et al, 2007) 20 mg/100 mL . Choi et al, 2006) 46-57 mg/100 mL , (Silkin and Silkina, 2005).…”

Section: Ast 및 Altmentioning

confidence: 99%

Physiological Stress Responses in Black Seabream Acanthopagrus schlegelii Subjected to Acute Hypoxia

Min¹,

Park²,

Myeong³

et al. 2013

Korean Journal of Fisheries and Aquatic Sciences

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Plasma glucose kinetics and tissue uptake in brown trout in vivo: effect of an intravascular glucose load

Cited by 86 publications

References 20 publications

Effects of food-deprivation and refeeding on the regulation and sources of blood glucose appearance in European seabass (Dicentrarchus labrax L.)

Effects of food-deprivation and refeeding on the regulation and sources of blood glucose appearance in European seabass (Dicentrarchus labrax L.)

Expression of rainbow trout glucose transporters GLUT1 and GLUT4 during in vitro muscle cell differentiation and regulation by insulin and IGF-I

Physiological Stress Responses in Black Seabream Acanthopagrus schlegelii Subjected to Acute Hypoxia

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