Plasma from obese children increases monocyte-endothelial adhesion and affects intracellular insulin signaling in cultured endothelial cells: Potential role of mTORC1-S6K1

Pietrantonio, Nadia Di; Palmerini, Carola; Pipino, Caterina; Baldassarre, M; Bologna, Giuseppina; Mohn, Angelika; Giannini, Cosimo; Lanuti, Paola; Chiarelli, Francesco; Pandolfi, Assunta; Pietro, Natalia Di

doi:10.1016/j.bbadis.2021.166076

Cited by 5 publications

(5 citation statements)

References 63 publications

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“…Insulin resistance leads to persistent hyperglycemia and is a risk factor intimately linked to atherosclerosis development. In an insulin-resistant setting, a reduced nitrite oxide (NO) bioavailability induces vascular damage, increasing the expression of adhesion molecules and inflammatory cytokines with the ability to promote monocyte recruitment and infiltration to the blood vessel wall [ 36 ]. It was our rationale behind estimating insulin resistance in our cohort of obese children, analyzing potential associations of monocyte subsets, expressing CCR2 with IMT and insulin resistance.…”

Section: Discussionmentioning

confidence: 99%

The CCR2+ Monocyte Subsets Increase in Obese Boys but Not Girls with Abnormally High Carotid Intima-Media Thickness: A Pilot Study

Garcés-Hernández

Pedraza-Escudero

Garibay-Nieto

et al. 2022

JCDD

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The differential contribution of monocyte subsets expressing the C-C chemokine receptor 2 (CCR2) to subclinical atherosclerosis in girls and boys is unclear. In this pilot study, we compared classical, intermediate, and nonclassical monocyte subsets expressing CCR2 in 33 obese children of both sexes aged 8 to 16 divided by carotid intima-media thickness (IMT), considering values above the 75th percentile (p75) as abnormally high IMT. Obesity was defined as body mass index above the 95th percentile according to age and sex. Flow cytometry analyses revealed that boys but not girls with IMT ≥ p75 displayed increased CCR2+ cell percentage and CCR2 expression in the three monocyte subsets, compared to boys with IMT < p75. The CCR2+ cell percentage and CCR2 expression in the three monocyte subsets significantly correlated with increased IMT and insulin resistance in boys but not girls, where the CCR2+ nonclassical monocyte percentage had the strongest associations (r = 0.73 and r = 0.72, respectively). The role of CCR2+ monocyte subpopulations in identifying an abnormally high IMT shows a marked sexual dimorphism, where boys seem to be at higher subclinical atherosclerosis risk than girls.

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Section: Discussionmentioning

confidence: 99%

The CCR2+ Monocyte Subsets Increase in Obese Boys but Not Girls with Abnormally High Carotid Intima-Media Thickness: A Pilot Study

Garcés-Hernández

Pedraza-Escudero

Garibay-Nieto

et al. 2022

JCDD

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“…HUVEC applications range from cardiovascular to metabolic as well as wound healing and angiogenesis-related diseases ( Medina-Leyte et al, 2020 ). In addition, Gestational Diabetes (GD)-HUVEC exposed in vivo even transiently (during pregnancy) to hyperglycemia, exhibit some epigenetics modifications leading to a durable pro-inflammatory phenotype ( Di Fulvio et al, 2014 ; Di Pietrantonio et al, 2021 ). HUVEC have been used to study the pro- and anti-atherogenic effect of several molecules in the early stages of atherosclerosis ( Di Tomo et al, 2015 ; Di Pietro et al, 2020 ).…”

Section: Mechanism Of Actionmentioning

confidence: 99%

“…While these methods allow to assess the general oxidative status, more integrative approaches evaluating potential antioxidant effects with higher sensitivity as well as the identification of permanent markers of oxidative stress are required ( Pipino et al, 2020 ). Therefore, the levels of DNA/RNA damage, lipid peroxidation, and protein oxidation/nitration such as nitrotyrosine expression associated with impaired vascular nitric oxide (NO) secretion and availability may give a more reliable scenario regarding alteration of the oxidative balance ( Di Fulvio et al, 2014 ; Alshabibi et al, 2018 ; Di Pietrantonio et al, 2021 ).…”

Section: Mechanism Of Actionmentioning

confidence: 99%

Perinatal derivatives: How to best characterize their multimodal functions in vitro. Part C: Inflammation, angiogenesis, and wound healing

Flores

Pipino

Jerman

et al. 2022

Front. Bioeng. Biotechnol.

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Perinatal derivatives (PnD) are birth-associated tissues, such as placenta, umbilical cord, amniotic and chorionic membrane, and thereof-derived cells as well as secretomes. PnD play an increasing therapeutic role with beneficial effects on the treatment of various diseases. The aim of this review is to elucidate the modes of action of non-hematopoietic PnD on inflammation, angiogenesis and wound healing. We describe the source and type of PnD with a special focus on their effects on inflammation and immune response, on vascular function as well as on cutaneous and oral wound healing, which is a complex process that comprises hemostasis, inflammation, proliferation (including epithelialization, angiogenesis), and remodeling. We further evaluate the different in vitro assays currently used for assessing selected functional and therapeutic PnD properties. This review is a joint effort from the COST SPRINT Action (CA17116) with the intention to promote PnD into the clinics. It is part of a quadrinomial series on functional assays for validation of PnD, spanning biological functions, such as immunomodulation, anti-microbial/anti-cancer activities, anti-inflammation, wound healing, angiogenesis, and regeneration.

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“…Gestational diabetes mellitus (GDM) later leads to an imbalance between pro- and anti-inflammatory/oxidative molecules that may support the development of early vascular senescence and endothelial dysfunction in offspring [ 53 ]. In general, the chronic state of low-grade inflammation caused by obesity is known to interfere with the normal transmission of insulin signaling, favoring the onset of type 2 DM and further propagating the state of chronic inflammation and vascular dysfunction even years before the diagnosis of diabetes [ 34 , 54 ]. Based on the above premises, the present study was undertaken to investigate the potential anti-inflammatory role of AEO and LEO on primary endothelial cell cultures isolated from the umbilical cord vein of females with gestational diabetes mellitus (GDM-HUVEC), which represent a valuable in vitro model for reproducing the in vivo pro-inflammatory vascular phenotype associated with chronic hyperglycemia [ 55 ].…”

Section: Introductionmentioning

confidence: 99%

Essential Oils from Mediterranean Plants Inhibit In Vitro Monocyte Adhesion to Endothelial Cells from Umbilical Cords of Females with Gestational Diabetes Mellitus

Schiavone

Romasco

Pietrantonio

et al. 2023

IJMS

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Essential oils (EOs) are mixtures of volatile compounds belonging to several chemical classes derived from aromatic plants using different distillation techniques. Recent studies suggest that the consumption of Mediterranean plants, such as anise and laurel, contributes to improving the lipid and glycemic profile of patients with diabetes mellitus (DM). Hence, the aim of the present study was to investigate the potential anti-inflammatory effect of anise and laurel EOs (AEO and LEO) on endothelial cells isolated from the umbilical cord vein of females with gestational diabetes mellitus (GDM-HUVEC), which is a suitable in vitro model to reproduce the pro-inflammatory phenotype of a diabetic endothelium. For this purpose, the Gas Chromatographic/Mass Spectrometric (GC-MS) chemical profiles of AEO and LEO were first analyzed. Thus, GDM-HUVEC and related controls (C-HUVEC) were pre-treated for 24 h with AEO and LEO at 0.025% v/v, a concentration chosen among others (cell viability by MTT assay), and then stimulated with TNF-α (1 ng/mL). From the GC-MS analysis, trans-anethole (88.5%) and 1,8-cineole (53.9%) resulted as the major components of AEO and LEO, respectively. The results in C- and GDM-HUVEC showed that the treatment with both EOs significantly reduced: (i) the adhesion of the U937 monocyte to HUVEC; (ii) vascular adhesion molecule-1 (VCAM-1) protein and gene expression; (iii) Nuclear Factor-kappa B (NF-κB) p65 nuclear translocation. Taken together, these data suggest the anti-inflammatory efficacy of AEO and LEO in our in vitro model and lay the groundwork for further preclinical and clinical studies to study their potential use as supplements to mitigate vascular endothelial dysfunction associated with DM.

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Plasma from obese children increases monocyte-endothelial adhesion and affects intracellular insulin signaling in cultured endothelial cells: Potential role of mTORC1-S6K1

Cited by 5 publications

References 63 publications

The CCR2+ Monocyte Subsets Increase in Obese Boys but Not Girls with Abnormally High Carotid Intima-Media Thickness: A Pilot Study

The CCR2+ Monocyte Subsets Increase in Obese Boys but Not Girls with Abnormally High Carotid Intima-Media Thickness: A Pilot Study

Perinatal derivatives: How to best characterize their multimodal functions in vitro. Part C: Inflammation, angiogenesis, and wound healing

Essential Oils from Mediterranean Plants Inhibit In Vitro Monocyte Adhesion to Endothelial Cells from Umbilical Cords of Females with Gestational Diabetes Mellitus

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