Plasma fibronectin levels in normal subjects

Allard, D; Rapin, J.R.; Jacqueson, A.; Freund, M.; Coulaud, J.M.; Labrousse, J

doi:10.1016/0049-3848(86)90158-1

Cited by 3 publications

(2 citation statements)

References 11 publications

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“…Densitometric analysis of the signal showed that the ratio between the test and control lines was proportional to the log of Fn (analyte) concentration ( Figures 6B,C ) over a range of concentrations between 0.625 and 10 ng/μL, consistent with simple visual comparison of control band density. These are much lower concentrations of Fn than those measured in the serum of healthy individuals (259–400 ng/μL; Allard et al, 1986 ). A suitable serum dilution would therefore adjust the concentration to the quantitative detection range of the assay.…”

Section: Resultsmentioning

confidence: 75%

Ff-nano, short functionalized nanorods derived from Ff (f1, fd, or M13) filamentous bacteriophage

et al. 2015

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F-specific filamentous phage of Escherichia coli (Ff: f1, M13, or fd) are long thin filaments (860 nm × 6 nm). They have been a major workhorse in display technologies and bionanotechnology; however, some applications are limited by the high length-to-diameter ratio of Ff. Furthermore, use of functionalized Ff outside of laboratory containment is in part hampered by the fact that they are genetically modified viruses. We have now developed a system for production and purification of very short functionalized Ff-phage-derived nanorods, named Ff-nano, that are only 50 nm in length. In contrast to standard Ff-derived vectors that replicate in E. coli and contain antibiotic-resistance genes, Ff-nano are protein-DNA complexes that cannot replicate on their own and do not contain any coding sequences. These nanorods show an increased resistance to heating at 70∘C in 1% SDS in comparison to the full-length Ff phage of the same coat composition. We demonstrate that functionalized Ff-nano particles are suitable for application as detection particles in sensitive and quantitative “dipstick” lateral flow diagnostic assay for human plasma fibronectin.

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Section: Resultsmentioning

confidence: 75%

Ff-nano, short functionalized nanorods derived from Ff (f1, fd, or M13) filamentous bacteriophage

et al. 2015

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“…6B) indicated a second order polynomial dependence between the signal intensity at the test line and analyte concentration over a range of concentrations between 1.25 ng/μL and 10 ng/μL. Concentration of Fn in the serum of healthy individuals is 259-400 ng/μL (Allard et al, 1986), Furthermore, variations of Fn concentration in disease are also within the range of quantitative detection of this assay (Choate and Mosher, 1983;Cembrowski and Mosher, 1984;Weller et al, 1988;Honest et al, 2002;Mosher, 2006;Eissa et al, 2010). Hence, suitably diluted samples of healthy and affected individuals can be quantitatively determined using this assay.…”

Section: Resultsmentioning

confidence: 97%

Ff-nano, Short Functionalized Nanorods Derived from Ff (f1, fd or M13) Filamentous Bacteriophage

Sattar

Bennett

Wen

et al. 2015

Preprint

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F-specific filamentous phage of Escherichia coli (Ff: f1, M13 or fd) are long thin filaments (860 nm x 6 nm). They have been a major workhorse in display technologies and bionanotechnology; however, some applications are limited by the high length-to-diameter ratio of Ff. Furthermore, use of functionalized Ff outside of laboratory containment is in part hampered by the fact that they are genetically modified viruses. We have now developed a system for production and purification of very short functionalized Ff-phage-derived nanorods, named Ff-nano, that are only 50 nm in length. In contrast to standard Ff-derived vectors that replicate in E. coli and contain antibiotic-resistance genes, Ff-nano are protein-DNA complexes that cannot replicate on their own and do not contain any coding sequences. These nanorods show an increased resistance to heating at 70 °C in 1 % SDS in comparison to the full-length Ff phage of the same coat composition. We demonstrate that functionalized Ff-nano particles are suitable for application as detection particles in sensitive and quantitative "dipstick" lateral flow diagnostic assay for human plasma fibronectin.

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Fibronectin in plasma and CSF: Evidence for its intrathecal synthesis

Gervais¹,

Schuller²

1991

Journal of the Neurological Sciences

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Plasma fibronectin levels in normal subjects

Cited by 3 publications

References 11 publications

Ff-nano, short functionalized nanorods derived from Ff (f1, fd, or M13) filamentous bacteriophage

Ff-nano, short functionalized nanorods derived from Ff (f1, fd, or M13) filamentous bacteriophage

Ff-nano, Short Functionalized Nanorods Derived from Ff (f1, fd or M13) Filamentous Bacteriophage

Fibronectin in plasma and CSF: Evidence for its intrathecal synthesis

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