Plasma exosomes characterization reveals a perioperative protein signature in older patients undergoing different types of on-pump cardiac surgery

Carrozzo, Alessandro; Casieri, Valentina; Silvestre, Dario Di; Brambilla, Francesca; Nitto, Emanuele De; Sardaro, Nicola; Papini, Gaia; Storti, Simona; Settanni, Giuseppina; Solinas, Marco; Mauri, Pierluigi; Paparella, Domenico; Lionetti, Vincenzo

doi:10.1007/s11357-020-00223-y

Cited by 22 publications

(21 citation statements)

References 65 publications

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“…Indeed, the literature available on protein profile in this topic is currently limited to extracellular vesicles studied indiscriminately as a pool collection of both microvesicles and exosomes [ 32 , 33 ]. Recently, Carrozzo and colleagues showed a different protein expression profile of plasma exosomes in older cardiac surgery patients before and after CABG or heart valve surgery (HSV) [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

“…Conversely to the higher level of circulating ceruloplasmin found in myocardial infarction patients, exosomes isolated from STEMI displayed a reduced level of ceruloplasmin, suggesting that exosome protein expression does not necessarily reflect circulating free-form levels of this positive acute-phase protein [ 20 , 39 ]. Interestingly, changes in plasma exosome-associated inflammatory proteins, including ceruloplasmin, have been recently reported also in CABG and HSV patients [ 9 ].…”

Section: Discussionmentioning

confidence: 99%

“…Exosomes are small extracellular vesicles (30–150 nm in diameter) originating from multivesicular bodies, actively released by different cell types and detectable in several body fluids, including plasma. Exosomes play a central role in mediating both local (paracrine) and distant (endocrine) intercellular communication, and by transferring their bioactive cargo (DNA, chemokines, messenger RNAs, microRNAs and proteins), they modulate the behavior of recipient cells [ 7 , 8 , 9 ]. The growing knowledge about exosomes has highlighted their involvement in nearly all aspects of physiology and pathology [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…The growing knowledge about exosomes has highlighted their involvement in nearly all aspects of physiology and pathology [ 4 ]. As exosomal release can be triggered by several stimuli associated with cellular stress [ 9 , 10 , 11 , 12 , 13 , 14 ], exosome secretion from platelets, vascular endothelial cells and brain cells, may be modulated in several pathological conditions, including myocardial infarction and stroke. Moreover, the content of exosomes, representing a “fingerprint” of the releasing cell and its metabolic status, might be a rich source of biomarkers for various disease states [ 4 , 9 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

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Plasma Exosome Profile in ST-Elevation Myocardial Infarction Patients with and without Out-of-Hospital Cardiac Arrest

Zara

Campodonico

Cosentino

et al. 2021

IJMS

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The identification of new biomarkers allowing an early and more accurate characterization of patients with ST-segment elevation myocardial infarction (STEMI) is still needed, and exosomes represent an attractive diagnostic tool in this context. However, the characterization of their protein cargo in relation to cardiovascular clinical manifestation is still lacking. To this end, 35 STEMI patients (17 experiencing resuscitated out-of-hospital cardiac arrest (OHCA-STEMI) and 18 uncomplicated) and 32 patients with chronic coronary syndrome (CCS) were enrolled. Plasma exosomes were characterized by the nanoparticle tracking analysis and Western blotting. Exosomes from STEMI patients displayed a higher concentration and size and a greater expression of platelet (GPIIb) and vascular endothelial (VE-cadherin) markers, but a similar amount of cardiac troponin compared to CCS. In addition, a difference in exosome expression of acute-phase proteins (ceruloplasmin, transthyretin and fibronectin) between STEMI and CCS patients was found. GPIIb and brain-associated marker PLP1 accurately discriminated between OHCA and uncomplicated STEMI. In conclusion, the exosome profile of STEMI patients has peculiar features that differentiate it from that of CCS patients, reflecting the pathophysiological mechanisms involved in STEMI. Additionally, the exosome expression of brain- and platelet-specific markers might allow the identification of patients experiencing ischemic brain injury in STEMI.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Plasma Exosome Profile in ST-Elevation Myocardial Infarction Patients with and without Out-of-Hospital Cardiac Arrest

Zara

Campodonico

Cosentino

et al. 2021

IJMS

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“…EVs can modulate target cells via their cargo, enriched by non-coding RNAs, such as microRNAs (miRNAs), proteins, lipids and other metabolites. Recently, plasma EVs have gained attention as new theranostic tool, given their protective and regenerative effects (such as in the case of mesenchymal progenitor stromal-EVs), as well as their possible diagnostic role even in older surgical patients [142] . Interestingly, endothelial and neural cells after brain injury can secrete EVs crossing the blood brain barrier, reaching peripheral organs and regulating systemic inflammation [143] .…”

Section: Potential Therapeutic Strategies: From Drugs To Functional Fmentioning

confidence: 99%

Understanding the heart-brain axis response in COVID-19 patients: A suggestive perspective for therapeutic development

Lionetti

Coppini

Gerbino

et al. 2021

Pharmacological Research

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In-depth characterization of heart-brain communication in critically ill patients with severe acute respiratory failure is attracting significant interest in the COronaVIrus Disease 19 (COVID-19) pandemic era during intensive care unit (ICU) stay and after ICU or hospital discharge. Emerging research has provided new insights into pathogenic role of the deregulation of the heart-brain axis (HBA), a bidirectional flow of information, in leading to severe multiorgan disease syndrome (MODS) in patients with confirmed infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Noteworthy, HBA dysfunction may worsen the outcome of the COVID-19 patients. In this review, we discuss the critical role HBA plays in both promoting and limiting MODS in COVID-19. We also highlight the role of HBA as new target for novel therapeutic strategies in COVID-19 in order to open new translational frontiers of care. This is a translational perspective from the Italian Society of Cardiovascular Researches.

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Mechanisms and therapeutic strategies of extracellular vesicles in cardiovascular diseases

Li,

Feng,

Zhou

et al. 2023

MedComm

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Cardiovascular disease (CVD) significantly impacts global society since it is the leading cause of death and disability worldwide, and extracellular vesicle (EV)‐based therapies have been extensively investigated. EV delivery is involved in mediating the progression of CVDs and has great potential to be biomarker and therapeutic molecular carrier. Besides, EVs from stem cells and cardiac cells can effectively protect the heart from various pathologic conditions, and then serve as an alternative treatment for CVDs. Moreover, the research of using EVs as delivery carriers of therapeutic molecules, membrane engineering modification of EVs, or combining EVs with biomaterials further improves the application potential of EVs in clinical treatment. However, currently there are only a few articles summarizing the application of EVs in CVDs. This review provides an overview of the role of EVs in the pathogenesis and diagnosis of CVDs. It also focuses on how EVs promote the repair of myocardial injury and therapeutic methods of CVDs. In conclusion, it is of great significance to review the research on the application of EVs in the treatment of CVDs, which lays a foundation for further exploration of the role of EVs, and clarifies the prospect of EVs in the treatment of myocardial injury.

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Plasma exosomes characterization reveals a perioperative protein signature in older patients undergoing different types of on-pump cardiac surgery

Cited by 22 publications

References 65 publications

Plasma Exosome Profile in ST-Elevation Myocardial Infarction Patients with and without Out-of-Hospital Cardiac Arrest

Plasma Exosome Profile in ST-Elevation Myocardial Infarction Patients with and without Out-of-Hospital Cardiac Arrest

Understanding the heart-brain axis response in COVID-19 patients: A suggestive perspective for therapeutic development

Mechanisms and therapeutic strategies of extracellular vesicles in cardiovascular diseases

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