Plasma estradiol and progesterone concentrations during the female reproductive cycle in a highly placentotrophic viviparous lizard, Mabuya sp.

Barbosa-Moyano, Heriberto; Rodríguez-Chaparro, Salomé; Santos, Rodrigo Luis Silva Ribeiro; Ramírez‐Pinilla, Martha Patricia

doi:10.1016/j.ygcen.2020.113530

Cited by 5 publications

(5 citation statements)

References 67 publications

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“…Therefore, it is not surprising that we found fluctuations in steroid hormones that correspond to the reproductive process, such as higher estradiol early in reproduction during vitellogenesis and higher progesterone during gravidity and right before and during nesting. Similar fluctuations are seen across species ( Weiss et al, 2002 ; Barbosa-Moyano et al, 2020 ; Hudson et al, 2020 ). Interestingly, we observed slight elevations in testosterone in the fall during post-reproduction relative to late reproduction in males of both subspecies.…”

Section: Discussionsupporting

confidence: 62%

Complex tourism and season interactions contribute to disparate physiologies in an endangered rock iguana

French

Webb

Wilcoxen

et al. 2022

Conservation Physiology

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To promote survival and fitness, organisms use a suite of physiological systems to respond to both predictable and unpredictable changes in the environment. These physiological responses are also influenced by changes in life history state. The continued activation of physiological systems stemming from persistent environmental perturbations enable animals to cope with these challenges but may over time lead to significant effects on the health of wildlife. In the present study, we tested how varying environmental perturbations driven by tourism and associated supplemental feeding affects the energetics, corticosterone and immunity of six discrete populations of the northern Bahamian rock iguana (Cyclura cychlura inornata and Cyclura cychlura figginsi). We studied populations within and outside the reproductive season and quantified tourist numbers during sample collection. Specifically, we measured clutch size, body condition, plasma energy metabolites, reactive oxygen species, baseline corticosterone concentrations and immune function of male and female iguanas from each population to address whether (i) disparate physiologies are emerging across a gradient of tourism and feeding, (ii) both subspecies respond similarly and (iii) responses vary with season/reproductive condition. We found significant effects of tourism level, season and their interaction on the physiology of both C. c. inornata and C. c. figginsi, supporting the idea that tourism is leading to the divergence of phenotypes. Specifically, we found elevated plasma energy metabolites, oxidative stress and a measure of innate immunity (bactericidal ability), but reduced corticosterone concentrations with increasing tourism in both subspecies of rock iguanas. These physiological metrics differ according to the level of tourism in both subspecies and persist across seasons despite variation with natural seasonal and reproductive changes. These findings suggest that anthropogenic disturbance results in disparate physiologies in northern Bahamian rock iguanas.

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Section: Discussionsupporting

confidence: 62%

Complex tourism and season interactions contribute to disparate physiologies in an endangered rock iguana

French

Webb

Wilcoxen

et al. 2022

Conservation Physiology

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“…The same behaviour is reported for Sceloporus virgatus (Weiss et al, 2002), Phrynosoma cornutum (Wack et al, 2008), Chameleo calyptratus (Kummrow et al, 2010), Salvator merianae (Zena et al, 2019) and Mabuya sp. (Barbosa-Moyano et al, 2020). ER, and PR presence was not related to the sex steroid concentrations, as reported in the lizard U. acanthinura (Hammouche et al, 2017).…”

Section: Discussionmentioning

confidence: 49%

“…In female lizards, E 2 promotes follicular development and the synthesis of vitellogenin (VTG) in the liver. This hormone increases with the onset of vitellogenesis and ovulation in various species (Jones, 2011;Ramírez-Pinilla et al, 2015;Barbosa-Moyano et al, 2020). The ovary is the principal source of E 2 to exert its multiple effects via receptors in different tissues (Contrò et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…The principal source of P 4 is the corpus luteum (Hosie et al, 2003;Martínez-Torres et al, 2003. This progestogen shows low concentrations during follicular development, a peak before ovulation and the highest concentrations near oviposition or during embryo development (Jones, 2011;Ramírez-Pinilla et al, 2015;Barbosa-Moyano et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

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Sex steroid receptors in the ovarian follicles of the lizard Sceloporus torquatus

Cruz-Cano

Sánchez-Rivera

Álvarez-Rodríguez

et al. 2023

Zygote

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Summary Estradiol and progesterone have been recognized as important mediators of reproductive events in the female mainly via binding to their receptors. This study aimed to characterize the immunolocalization of the estrogen receptor alfa (ERα), estrogen receptor beta (ERβ) and progesterone receptor (PR) in the ovarian follicles of the lizard Sceloporus torquatus. The localization of steroid receptors has a spatio-temporal pattern that depends on the stage of follicular development. The immunostaining intensity of the three receptors was high in the pyriform cells and the cortex of the oocyte of previtellogenic follicles. During the vitellogenic phase, the granulosa and theca immunostaining was intense even with the modification of the follicular layer. In the preovulatory follicles, the receptors were found in yolk and additionally, ERα was also located in the theca. These observations suggest a role for sex steroids in regulating follicular development in lizards, like other vertebrates.

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“…Thus, the absence of potential calcium resources due to the reduction of the eggshell, together with the precocious shutdown of vitellogenesis, provides a scenario where the only source of calcium (and virtually all nutrients) in Mabuya, is direct transfer from the uterine tissues (Ramírez-Pinilla, 2006;Ramírez-Pinilla et al, 2011). From early gestation, the placenta of Mabuya transfers water, glucose, lipids (cholesterol, vitamin E and fatty acids), proteins (related to metabolism, signaling, progesterone synthesis) and some ions including potassium, sodium, magnesium and calcium, and supports processes such as gas exchange (Barbosa-Moyano et al, 2020;Duarte-Méndez et al, 2018;Hernández-Díaz et al, 2017;Ramírez-Pinilla et al, 2011;Wooding et al, 2010). Later in gestation, a more specialized allantoplacenta is established, with a more complex arrangement of the maternal-fetal tissues that gives rise to specializations like the placentome, paraplacentome, chorionic areolas, absorptive plaques (structures associated with the exchange of nutrients) and respiratory segments Ramírez-Pinilla, 2001, 2003;Leal and Ramírez-Pinilla, 2008;Blackburn and Flemming, 2009).…”

Section: Introductionmentioning

confidence: 99%

Parallel evolution of placental calcium transfer in the lizard Mabuya and eutherian mammals

Hernández-Díaz

Leal

Ramírez‐Pinilla

2021

Journal of Experimental Biology

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An exceptional case of parallel evolution between lizards and eutherian mammals occurs in the evolution of viviparity. In the lizard genus Mabuya, viviparity provided the environment for the evolution of yolk-reduced eggs and obligate placentotrophy. One major event that favored the evolution of placentation was the reduction of the eggshell. As with all oviparous reptiles, lizard embryos obtain calcium from both the eggshell and egg yolk. Therefore, the loss of the eggshell likely imposes a constraint for the conservation of the egg yolk, which can only be obviated by the evolution of alternative mechanisms for the transport of calcium directly from the mother. The molecular and cellular mechanism employed to solve these constraints, in a lizard with only a rudimentary eggshell such as Mabuya, are poorly understood. Here, we used RT-qPCR on placental and uterine samples during different stages of gestation in Mabuya, and demonstrate that transcripts of the calcium transporters trpv6, cabp28k, cabp9k, and pmca are expressed and gradually increase in abundance through pregnancy stages, reaching their maximum expression when bone mineralization occurs. Furthermore, CABP28K/9K proteins were studied by immunofluorescence, demonstrating its expression in specific regions of the mature placenta. Our results indicate that the machinery for calcium transportation in the Mabuya placenta was co-opted from other tissues elsewhere in the vertebrate bodyplan. Thus, the calcium transportation machinery in the placenta of Mabuya evolved in parallel with the mammalian placenta by redeploying the expression of similar calcium transporter genes.

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Plasma estradiol and progesterone concentrations during the female reproductive cycle in a highly placentotrophic viviparous lizard, Mabuya sp.

Cited by 5 publications

References 67 publications

Complex tourism and season interactions contribute to disparate physiologies in an endangered rock iguana

Complex tourism and season interactions contribute to disparate physiologies in an endangered rock iguana

Sex steroid receptors in the ovarian follicles of the lizard Sceloporus torquatus

Parallel evolution of placental calcium transfer in the lizard Mabuya and eutherian mammals

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