Plasma endocannabinoids and cannabimimetic fatty acid derivatives are altered in gastroparesis: A sex‐ and subtype‐dependent observation

Bashashati, Mohammad; Leishman, Emma; Bradshaw, Heather B.; Sigaroodi, Solmaz; Tatro, Eric; Bright, Tamis; McCallum, Richard W.; Sarosiek, Irene

doi:10.1111/nmo.13961

Cited by 10 publications

(8 citation statements)

References 55 publications

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“…These techniques can be applied to different samples (plasma, brain regions, skeletal muscle, adipose tissues, etc.). For example, using this technique, AEA, palmitoyl-ethanolamide (PEA), oleoylethanolamide (OEA), 2-AG and its isomer 1-AG [78], and N-acyl-ethanolamines (NAEs) and 2-monoacylglycerols (2-MAGs), congeners of AEA and 2-AG [82,83], have been determined in plasma. Similarly, eCBs and their derivatives have been measured in rat liver tissue [84] and AEA metabolite formation in kidney tissue samples [85] by HPLC-MS/MS.…”

Section: Alterations In Endocannabinoid Ligandsmentioning

confidence: 99%

Molecular Alterations of the Endocannabinoid System in Psychiatric Disorders

Navarro

Gasparyan

Navarrete

et al. 2022

IJMS

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The therapeutic benefits of the current medications for patients with psychiatric disorders contrast with a great variety of adverse effects. The endocannabinoid system (ECS) components have gained high interest as potential new targets for treating psychiatry diseases because of their neuromodulator role, which is essential to understanding the regulation of many brain functions. This article reviewed the molecular alterations in ECS occurring in different psychiatric conditions. The methods used to identify alterations in the ECS were also described. We used a translational approach. The animal models reproducing some behavioral and/or neurochemical aspects of psychiatric disorders and the molecular alterations in clinical studies in post-mortem brain tissue or peripheral tissues were analyzed. This article reviewed the most relevant ECS changes in prevalent psychiatric diseases such as mood disorders, schizophrenia, autism, attentional deficit, eating disorders (ED), and addiction. The review concludes that clinical research studies are urgently needed for two different purposes: (1) To identify alterations of the ECS components potentially useful as new biomarkers relating to a specific disease or condition, and (2) to design new therapeutic targets based on the specific alterations found to improve the pharmacological treatment in psychiatry.

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Section: Alterations In Endocannabinoid Ligandsmentioning

confidence: 99%

Molecular Alterations of the Endocannabinoid System in Psychiatric Disorders

Navarro

Gasparyan

Navarrete

et al. 2022

IJMS

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“…eCB ligands and related endolipids are not limited to the brain, and from a translational standpoint measuring endolipids in biological specimens that are less invasive to sample, such as plasma, could be particularly informative. Already, we have shown sex differences in endolipids in plasma of patients with gastroparesis 22 . Likewise, serum collected from cannabis users shows increased palmitic acid, oleic acid, 2-AG, and PEA, but not AEA or OEA compared to non-cannabis users 23 , which illustrates that plasma endolipids are responsive to changes in systemic CB pharmacology.…”

Section: Introductionmentioning

confidence: 99%

Plasma and Liver Lipids are Differentially Regulated After Cannabinoid Treatments in Male and Female Mice

Johnson

Bosquez-Berger

Bentz

et al. 2023

Preprint

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Cannabinoids (CBs) have sex-dependent behavioral and physiological effects and modulate lipids across the body. To understand how some of these sex differences may be due to differences how both phyto- and endocannabinoids are regulated in the liver and plasma, male and female CD1 mice were administered 10 mg/kg i.p. of cannabidiol (CBD), ∆9-tetrahydrocannabinol (THC), or THC+CBD then core blood and liver tissue were collected after 2 hours. Lipids were extracted from both liver and plasma, and samples were screened via HPLC/MS/MS for ∆9-tetrahydrocannabinol (THC), metabolites 11-OH-THC and 11-COOH-THC, cannabidiol (CBD), metabolites 7-OH-CBD and 7-COOH-CBD, ~70 endolipids including the endocannabinoids, N-arachidonoyl ethanolamine (Anandamide; AEA) and 2-sn-arachidonoyl glycerol (2-AG). Structural analogs to AEA (e.g. lipoamino acids, lipoamines) and 2-acyl glycerols) as well as free fatty acids and prostaglandins were also evaluated. Results show that at 2 hours post injection levels of CBs demonstrate key differences between males and females in both plasma and liver, and that these differences vary when co-administered as opposed to administered alone. Illustrating a link between liver and plasma, directionality of CB differences are similar between the two tissue types as a function of both sex and CB treatment. By contrast, endolipids had very different profiles as a function of sex, CB administration, and tissue type. Importantly, there are baseline differences between male and female mice in endocannabinoids and related lipids, which likely impact how CB administration modulates these endolipids. These data illustrate the complexity of outcomes of CB treatment between males and females on circulating CBs and endolipids and highlight the need to consider these factors when evaluating efficacy of CB drug treatments or usage.

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“…11,12 These enzymes are also involved in the processing of a wide range of endogenous lipids with cannabimimeticlike properties; however, there are limited available data on their physiological roles in the development and progression of human disorders, including DGBI. 13 Given the importance of the eCB system in the pathophysiology of CVS and their role as a modulator of the autonomic nervous system, which is dysregulated in CVS, we tested the hypothesis that CVS is associated with changes in circulating eCB and related lipid signaling molecules, and these changes are potentiated by the cephalic-vagal stimulation during sham feeding.…”

Section: Introductionmentioning

confidence: 99%

Plasma endocannabinoids and cannabimimetic fatty acid derivatives are altered in cyclic vomiting syndrome: The effects of sham feeding

Bashashati

Bradshaw

Johnson

et al. 2023

Journal of Investigative Medicine

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Cyclic vomiting syndrome (CVS) is an underdiagnosed disorder of the gut-brain interaction. Our understanding of the pathophysiology of CVS is evolving. Here, we tested the hypotheses that: (1) the levels of endocannabinoids and related lipids are altered in CVS, and (2) cephalic-vagal stimulation drive changes in endolipid levels. Ten adult patients with CVS and 8 healthy controls were included. Indirect measurements of parasympathetic (RFa) functions were performed with spectral analysis of heart rate variability and respiratory activity. Plasma levels of endocannabinoids and related lipids were measured at baseline and during a sham feeding. Values are reported as mean ± SEM and compared using t-test or ANONA. CVS patients had a lower parasympathetic tone and response to Valsalva maneuver and deep breathing than the controls. The baseline 2-Arachidonoylglycerol (2-AG) had a significantly higher concentration in CVS (5.9e-008 ± 3.7e-008 mole/L) than control (3.7e-008 ± 1.3e-008 mole/; p<0.05). Sham feeding did not change the concentration of 2-AG. 2-linoleoylglycerol and 2-oleoylglycerol were significantly higher in CVS than control and did not change with sham feeding. Levels of N-acyl ethanolamines, including Anandamide (AEA), were not different in CVS vs. control. After sham feeding, AEA showed a trend toward increasing (P=0.08) in CVS, but not in control. With sham feeding, palmitoyl ethanolamine significantly increased in both CVS and control groups; oleoyl ethanolamine in CVS only, and stearoyl ethanolamine in the control group. Levels of endocannabinoids and related lipids are altered in CVS patients. Sham feeding affects endogenous signaling lipids in a disease and time-dependent manner.

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Plasma endocannabinoids and cannabimimetic fatty acid derivatives are altered in gastroparesis: A sex‐ and subtype‐dependent observation

Cited by 10 publications

References 55 publications

Molecular Alterations of the Endocannabinoid System in Psychiatric Disorders

Molecular Alterations of the Endocannabinoid System in Psychiatric Disorders

Plasma and Liver Lipids are Differentially Regulated After Cannabinoid Treatments in Male and Female Mice

Plasma endocannabinoids and cannabimimetic fatty acid derivatives are altered in cyclic vomiting syndrome: The effects of sham feeding

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