Plasma Dynamic Viscosity Determined by NMR

Guevara, Manuel Arsenio Lores; Torres, Yulianela Mengana; Naranjo, Juan Carlos García; Suárez, Norberto Rodríguez; Beyríes, Lidia Clara Suárez; Feliu, María A. Marichal; Boada, Teresa Simón; Reyes, Inocente Clemente Rodríguez; Philippé, Jan

doi:10.1007/s00723-018-1026-x

Cited by 5 publications

(13 citation statements)

References 14 publications

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“…The CMCh solution had the smallest T 2 distribution width. The peak is shifted to lower T 2 values compared to water due to the higher viscosity of the CMCh solution (Guevara et al 2018). The two inhomogeneous hydrogel networks (DS Ald ¼ 0:20, DS Ald ¼ 0:28) showed a broad and bimodal distribution.…”

Section: Homogeneity and Permeability Of The Hydrogelsmentioning

confidence: 96%

Development of hydrogels based on oxidized cellulose sulfates and carboxymethyl chitosan

et al. 2019

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Cellulose or chitosan represent highly abundant biopolymers possessing excellent biocompatibility that is required in tissue engineering. Both, cellulose and chitosan can be used to form hydrogels that can replace soft human tissues like cartilage. Hence, we developed here an approach to oxidize cellulose after sulfation, which was then crosslinked with carboxymethyl chitosan (CMCh). Sulfation was performed either by direct or acetosulfation reaching different sulfation degrees of DS Sulf = 0.8-2.0. Subsequently oxidation of cellulose sulfate (CS) was performed with sodium periodate, which yielded aldehyde contents of DS Ald = 0.1-0.3. Since oxidation requires the presence of vicinal hydroxyl groups in the anhydroglucose unit (AGU) of CS, higher sulfation degree as obtained by direct sulfation including hydroxyl groups at C2 and C3-position yielded lower aldehyde contents. On the contrary, regioselective sulfation at C6-position by acetosulfation was more suitable to achieve higher oxidation degrees of CS. Consequently, hydrogel formation obtained by chemical crosslinking of oxidized cellulose sulfate (oCS) with CMCh was fast within seconds when oxidation degree was high, but sulfation degree low. Moreover gel formation lasted almost 24 h when sulfation degree was high. It could also be shown that hydrogels based on oCS with a DS Ald of 0.28 or higher were stable for 25 days when incubated in phosphate-buffered saline (PBS) or Dulbeccos modified Eagle medium (DMEM). Studies with pH dependent fluorescent tracer molecules could show that the intrinsic pH value in hydrogels was slightly acidic ($ pH 6.4) when they were incubated in PBS at pH 7.4. Mass transfer and homogeneity of the gel network was studied by NMR finding that diffusion of water molecules was not hindered inside the hydrogels.

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Section: Homogeneity and Permeability Of The Hydrogelsmentioning

confidence: 96%

Development of hydrogels based on oxidized cellulose sulfates and carboxymethyl chitosan

et al. 2019

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“…The increasing of the blood dynamic viscosity (b) provokes negative effects in uts hemodynamic, including the Blood Hiperviscosity Syndrome (BHS), and can affects several diseases as: polycythemia, Waldenström macroglobulinemia, multiple myeloma (MM), leukemia, rheumatoid arthritis, asthma, STROKE and Sickle Cell Anemia (SCA) [1][2][3][4][5]. Three main parameters determine the behavior of b: the hematocrit (H) and the absolute dynamic viscosities of the blood plasma (p) and the intracellular hemoglobin (Hb) solution (Hb) [6]:…”

Section: Introductionmentioning

confidence: 99%

“…p and Hb can be determined using capillary, falling body or rotational viscometers [1,2,7]. However, these viscometers require a minimum volume of the sample of 2 ml and must be washed after each determination; which makes the process of dynamic viscosity () evaluation invasive, cumbersome and time consuming [1].…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the dynamic viscosity in hemoglobin solutions using proton magnetic relaxation

Cruz,

Torres,

Naranjo

et al. 2024

Preprint

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A Proton Magnetic Relaxation method to determine the absolute dynamic viscosity in samples of hemoglobin (Hb) solutions (ηHb) is presented. The approach is based on the inverse relationship between this physical parameter and the transverse proton magnetic relaxation time (T2). The Hb samples were voluntary obtained from whole blood of healthy individuals and patients, and processed by classical methods (centrifugation, decanting and freezing-thawing cycles).The Carr-Purcell-Meiboon-Gill pulse sequence was employed to determine T2 in a Tecmag Magnetic Resonance console coupled to a magnet of 0. 095 T and the temperature of measurement was 293 K. A theoretical expression was derived; which properly describes the experimental behavior of the transverse proton magnetic relaxation rate (R2) with ηHb. This equation was successfully used to estimate ηHb in samples belonging to 10 healthy individuals considering that the values obtained statistically match with those measured using an Ostwald viscometer. The potential utility, for medical applications, of this proton magnetic resonance based method was observed estimating ηHb in samples belonging to 46 sickle cell disease patients; being the estimated values bigger than those obtained for the case of healthy individuals due to the increasing in the intermolecular interactions inside the hemoglobin S solution, which is provoked by the presence of HbS polymers and fragments of this type of polymers. To use this method a special care must be taken with the temperature and the frequency of resonance used, as well as with the homogeneity of the static magnetic system. Additionally; the presence inside the sample of external amount of water, paramagnetic compounds and/or other biological materials must be avoided.

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“…It is particularly important in pathologies as the Waldenström Macroglobulinemia (WM) and Multiple Myeloma (MM), where the blood concentration of monoclonal immunoglobulin (IgM, IgG and IgA) is increased [2]. Nevertheless; the traditional methods to determine S have a limited clinical application because of the sample volume needed, the necessity of to wash the viscometer to be used in different patients and the additional determinations required to convert the kinematic viscosity () in absolute dynamic viscosity () [1,3].…”

Section: Introductionmentioning

confidence: 99%

Dynamic viscosity of blood serum determined using proton magnetic relaxation

Torres,

Guevara,

Milán

et al. 2023

Preprint

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An experimental procedure, based on proton magnetic relaxation, is presented to determine the absolute dynamic viscosity in blood serum (hS). The blood serum samples were obtained voluntary from whole blood of healthy individuals and patients, and processed by classical methods (centrifugation and decanting). The Carr-Purcell-Meiboon-Gill pulse sequence was employed to determine the transverse proton magnetic relaxation time (T2) in a Tecmag Magnetic Resonance console coupled to a magnet of 0. 095 T and the temperature of measurement was 293 K. A theoretical linear behavior of the transverse proton magnetic relaxation rate (1/T2) as a function of hS was obtained after the consideration of blood serum as an extremely diluted solution of albumin and globulins, and assuming a fast exchange of water molecules between the bound phase and the solvent. A value of hS= 1.29±0.07 mPa s was obtained in samples belonging to 20 voluntary healthy individuals, which statistically match with the value obtained using the Ostwald viscometer for the same samples (hS= 1.32±0.04 mPa s, P=0.104319>0.05, a=0.05). The potential medical utility of the presented proton magnetic resonance procedure was demonstrated in patients with Multiple Myeloma (24) and Sickle Cell Disease (34), in which hS resulted increased with values of 1.40±0.18 mPa s (P=0.0137509<0.05, a=0.05) and 1.36±0.10 mPa s (P=0.00809615<0.05, a=0.05) respectivelly.

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Plasma Dynamic Viscosity Determined by NMR

Cited by 5 publications

References 14 publications

Development of hydrogels based on oxidized cellulose sulfates and carboxymethyl chitosan

Development of hydrogels based on oxidized cellulose sulfates and carboxymethyl chitosan

Evaluation of the dynamic viscosity in hemoglobin solutions using proton magnetic relaxation

Dynamic viscosity of blood serum determined using proton magnetic relaxation

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