Plasma drop and thin-film revealed distinguished molecular structure in pre-eclampsia: An investigation using synchrotron Fourier-transform infrared microspectroscopy

Dahabiyeh, Lina A.; Mansour, Randa S.H.; Darwish, Wesam; Saleh, Shawqi; Kamel, Gihan

doi:10.1016/j.jpba.2022.114981

Cited by 2 publications

(2 citation statements)

References 33 publications

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“…The collected raw spectra were processed using the multivariate statistical analysis software the Unscrambler X 10.4 (CAMO Analytics, Norway), which was used as previously described [ 48 , 49 , 50 ]. Briefly, the absorption spectra in the two identified spectral regions, lipid CH stretching (2800–3050 cm −1 ) and the protein (amide I and II)-carbonyl vibrations (1485–1855 cm −1 ), were baseline-corrected and smoothed with a 7-point window using the Savitzky–Golay smoothing algorithm (2 nd polynomial order).…”

Section: Methodsmentioning

confidence: 99%

Synchrotron-Radiation-Based Fourier Transform Infrared Microspectroscopy as a Tool for the Differentiation between Staphylococcal Small Colony Variants

et al. 2022

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Small colony variants (SCVs) are clinically significant and linked to persistent infections. In this study, synchrotron-radiation-based Fourier transform infrared (SR-FTIR) is used to investigate the microspectroscopic differences between the SCVs of Staphylococcus aureus (S. aureus) and diabetic foot Staphylococcus epidermidis (S. epidermidis) in two main IR spectral regions: (3050–2800 cm−1), corresponding to the distribution of lipids, and (1855–1500 cm−1), corresponding to the distribution of protein amide I and amide II and carbonyl vibrations. SR-FTIR successfully discriminated between the two staphylococcal species and between the SCV and the non-SCV strains within the two IR spectral regions. Combined S. aureus SCVs (SCVhMu) showed a higher protein content relative to the non-SCV wild type. Complemented S. aureus SCV showed distinguishable differences from the SCVhMu and the wild type, including a higher content of unsaturated fatty acids. An increase in the CH2/CH3 ratio was detected in S. epidermidis SCV samples compared to the standard control. Protein secondary structure in standard S. epidermidis and SCVs consisted mainly of an α-helix; however, a new shoulder at 1635 cm−1, assigned to β-sheets, was evident in the SCV. In conclusion, SR-FTIR is a powerful method that can discriminate between staphylococci species and to differentiate between SCVs and their corresponding natural strains.

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Section: Methodsmentioning

confidence: 99%

Synchrotron-Radiation-Based Fourier Transform Infrared Microspectroscopy as a Tool for the Differentiation between Staphylococcal Small Colony Variants

et al. 2022

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“…Pregnancy-induced hypertension (PIH) is one of the leading causes of maternal and perinatal morbidity and mortality [1] . Preeclampsia (PE) is a serious complication of pregnancy that was characterized by new-onset hypertension around the 20th gestational week (GW) with or without proteinuria [2] and may complicate up to 5% of pregnancies worldwide [3] .…”

Section: Introductionmentioning

confidence: 99%

The Relation between Deregulated Immune Milieu and Atherogenic Lipids Might Underlie the Development of Pregnancy-induced Hypertensive Disorders

Zaher

Rageh

Morsy

2023

Evidence Based Women's Health Journal

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Objectives: Evaluation of the relation between plasma lipoprotein(a) (LPA) and serum tumor necrosis factor-α (TNF-α) levels estimated in blood samples (S1 sample) obtained at the 6th gestational week (GW; T1 time) and the development of preeclampsia (PE) as judged by blood pressure measures at the time of diagnosis of PE (T2 time). Patients and Methods: 140 newly pregnant women gave S1 sample at T1 time; during pregnancy 19 women developed early-onset PE (EOPE) and 51 women developed late-onset PE (LOPE); 16 women developed severe PE (SPE) and 54 women developed mild PE (MPE), while 70 women were normotensive (NT) till the end of pregnancy. At T2 time, all patients gave S2 sample for ELISA estimation of plasma LPA and serum TNF-α level in both samples. Results: Serum TNF-α and plasma LPA levels were significantly higher in all S2 than S1 samples, in both samples of PE than NT women, and in both samples of women who developed EOPE and/or SPE than in samples of women who developed LOPE and/or MPE, respectively. Regression analysis of T1 data defined high body mass index; BMI (β=0.162, P=0.028), high S1 levels of TNF-α (β=0.424, P<0.001), and LPA (β=0.314, P<0.001) as predictors for development of PE as judged by SBP measures at T2 time. Correlation analysis showed a positive significant (P<0.001) correlation between at-T2-SBP measures with at-T1 BMI and S1 levels of TNF-α and LPA with a positive significant correlation between levels of both variables and with at-T1 BMI. Conclusion: High serum levels of TNF-α and plasma LPA levels early in pregnancy could predict the development of PE and its severity.

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Plasma drop and thin-film revealed distinguished molecular structure in pre-eclampsia: An investigation using synchrotron Fourier-transform infrared microspectroscopy

Cited by 2 publications

References 33 publications

Synchrotron-Radiation-Based Fourier Transform Infrared Microspectroscopy as a Tool for the Differentiation between Staphylococcal Small Colony Variants

Synchrotron-Radiation-Based Fourier Transform Infrared Microspectroscopy as a Tool for the Differentiation between Staphylococcal Small Colony Variants

The Relation between Deregulated Immune Milieu and Atherogenic Lipids Might Underlie the Development of Pregnancy-induced Hypertensive Disorders

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