Plasma‐Driven in Situ Production of Hydrogen Peroxide for Biocatalysis

Abstract

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Microscale Atmospheric Pressure Plasma Jet as a Source for Plasma‐Driven Biocatalysis

“…[3] One approach makes use of atmospheric pressure plasmas. [4] In general, plasmas are generated by high electric fields, accelerating free electrons that collide with atoms or molecules in the gas phase, which leads to the formation of metastable as well as excited species and ions. [5] The shortliving reactive species react with each other or with surrounding gas atoms or molecules to eventually yield less reactive species.…”

“…We previously showed that the H 2 O 2 in plasma-treated buffers can drive biocatalysis with the in vitro-evolved, recombinant UPO from Agrocybe aegerita (rAaeUPO). [4] When enzyme and substrate were treated together with plasma, however, the yield of product was significantly reduced as compared to a catalysis scheme in which plasma treatment and subsequent biocatalysis were uncoupled. Since the enzyme was quickly inactivated by plasma treatment with a dielectric barrier discharge (DBD), immobilization was used to prolong the lifetime of the enzyme.…”

“…[12] This enzyme showed remarkable turnover numbers (TONs) and enantioselectivity in previous studies and was used in conjunction with plasma before. [4] After treating 5 mL of buffer with the μAPPJ for 15 min, ETBE and rAaeUPO were added and allowed to react for 15 min. Both H 2 O 2 produced with the μAPPJ and a diluted H 2 O 2 stock solution with the same concentration as in plasma-treated buffer yielded approx.…”

Mass Production of Plasma Activated Water: Case Studies of Its Biocidal Effect on Algae and Cyanobacteria

“…In agriculture, exposure to PAW can lead to enhanced seed germination and growth of plants [9] in addition to its strong bactericidal effect [10]. Further applications of discharge plasmas generated in liquids include chemical synthesis [11][12][13], nanoparticle synthesis [14][15][16], destruction of pollutants in wastewaters [17][18][19][20][21][22], polymer surface treatment [23][24][25], etc. Nevertheless, the main hindrance remains in place even after two decades of intensive research effort: What is an effective method for large-volume liquid treatment?…”

“…Introduction of the gas-phase allows reduction of breakdown strength to technically more feasible values of 10 kV/cm. Typical representatives of such an approach include plasma jets submerged in liquid [7,11,27]; discharges generated above [10,13] or from the liquid surface [25], discharges in liquid aerosol [3]; or discharges initiated inside the gas micro-bubbles introduced [21] or formed inside the liquid volume [18,24]. Still, none of these methods of discharge plasma generation can deliver a sufficiently high and cost-effective throughput of plasma-treated liquid (see Table 1).…”