Plasma‐driven biocatalysis: In situ hydrogen peroxide production with an atmospheric pressure plasma jet increases the performance of OleT_JE when compared to adding the same molar amount of hydrogen peroxide in bolus

Abstract

Enzymes like fatty acid peroxygenase OleTJE are desirable enzymes for the industry. While they require inexpensive hydrogen peroxide for activity, the same hydrogen peroxide also causes overoxidation of their reactive heme center. Here, we generate hydrogen peroxide slowly in situ using the Cooperation in Science and Technology (COST)‐Jet, an atmospheric pressure plasma jet, to avoid overoxidizing OleTJE. The COST‐Jet was operated in helium with a water admixture to provide hydrogen peroxide for OleTJE activity. This helium/water admixture produced the highest enzyme turnover numbers after 2 min of treatment. These turnover numbers were even superior to using an equimolar amount of hydrogen peroxide to treat the enzymes exogenously, showing that this plasma source can provide a reliable amount of reaction mediator to support OleTJE activity.

Nitrogen fixation and H2O2 production by an atmospheric pressure plasma jet operated in He–H20–N2–O2 gas mixtures

“…Plasma‐treated liquids are important in various fields such as plasma medicine, [ 1,2 ] bacteria inactivation, [ 3 ] plasma‐assisted agriculture [ 4 ] or plasma‐driven biocatalysis. [ 5,6 ] In all fields, atmospheric pressure plasma jets (APPJs) are suitable for generating reactive species and introducing them into liquids under very good control. For example, in plasma‐driven biocatalysis, the use of an APPJ to generate $${{\rm{H}}}_{2}{{\rm{O}}}_{2}$$ and introduce it into a liquid in which biological enzymes perform a desired reaction has proven to be suitable.…”

Validation of in situ diagnostics for the detection of OH and H2O2 in liquids treated by a humid atmospheric pressure plasma jet

“…Possible applications are very diverse, ranging from nitrogen fixation, [1,2] plasma medicine, [3,4] bacteria inactivation, [5,6] to plasma-driven biocatalysis. [7][8][9] In these applications, plasma-liquid interactions play an important role. [10,11] In plasma-liquid arrangements where the plasma is not ignited in the liquid but its effluent is in contact with the liquid, as is often the case with plasma jets, reactive oxygen and nitrogen species generated in the plasma and transported into the liquid are the main driver for the desired reactions.…”

2D-imaging of absolute OH and H2O2 profiles in a He–H2O nanosecond pulsed dielectric barrier discharge by photo-fragmentation laser-induced fluorescence

“…Compared to OH, studies in quantifying H 2 O 2 profiles in a plasma have been much less prevalent. Investigations of H 2 O 2 produced in plasma-liquid interactions have mostly been limited to the liquid phase, where H 2 O 2 concentrations are measured ex-situ by sampling the effluent and using colorimetric methods [30]. IR-absorption spectroscopy has been used to measure gas-phase H 2 O 2 concentrations in other applications, such as shock tube studies [31] but yielded only line of sight measurements.…”