“…[8], using tyraminc as substrate at optimal pH. The incubation mixture (final volume 1.0 ml) was chosen according to the original method: diluted plasma (50 ¡¿1 diluted with distilled water to 400 ¡¿1); 1 M sodium acetate, buffered at pH 5.0, 200 ¡¿I; 0.2 M N-ethylmaleimide, 150 (¿1; 0.2 M Na-fumarate, 50 ¡/.I; 0.02 M pargyline, 50 ¡¿1:0.2 M ascorbic acid, 50 ¡¿I; catalase ( I mg/ml), 50 ¡¿I containing 1,500 units, and 0.4 M tyramine-HCI, 50 u.1.…”