Plasma Disappearance Rate of Indocyanine Green in Liver Dysfunction

Faybik, Peter; Hetz, Hubert

doi:10.1016/j.transproceed.2006.01.049

Cited by 82 publications

(61 citation statements)

References 12 publications

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“…Collectively, while these data suggest that bile acid is synthesized and secreted, it would be important to confirm the presence of bile acids via HPLC analysis. Uptake and efflux of ICG is clinically used to evaluate liver functions [38,39]. The monolayer HLC showed uptake of ICG and complete efflux occurred within 24 h. A similar profile in the uptake and efflux of ICG was also seen with the encapsulated HLC.…”

Section: Properties Of Encapsulated Hepatocyte-like Cells 871mentioning

confidence: 76%

Hepatocyte-Like Cells Derived from Human Amniotic Epithelial Cells Can Be Encapsulated Without Loss of Viability or Function In Vitro

Vaghjiani

Vaithilingam

Saraswati

et al. 2014

Stem Cells and Development

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Placenta derived human amniotic epithelial cells (hAEC) are an attractive source of stem cells for the generation of hepatocyte-like cells (HLC) for therapeutic applications to treat liver diseases. During hAEC differentiation into HLC, they become increasingly immunogenic, which may result in immune cell-mediated rejection upon transplantation into allogeneic recipients. Placing cells within devices such as alginate microcapsules can prevent immune cell-mediated rejection. The aim of this study was to investigate the characteristics of HLC generated from hAEC and to examine the effects of encapsulation on HLC viability, gene expression, and function. hAEC were differentiated for 4 weeks and evaluated for hepatocyte-specific gene expression and function. Differentiated cells were encapsulated in barium alginate microcapsules and cultured for 7 days and the effect of encapsulation on cell viability, function, and hepatocyte related gene expression was determined. Differentiated cells performed key functions of hepatocytes including urea synthesis, drug-metabolizing cytochrome P450 (CYP)3A4 activity, indocyanine green (ICG) uptake, low-density lipoprotein (LDL) uptake, and exhibited glutathione antioxidant capacity. A number of hepatocyte-related genes involved in fat, cholesterol, bile acid synthesis, and xenobiotic metabolism were also expressed showing that the hAEC had differentiated into HLC. Upon encapsulation, the HLC remained viable for at least 7 days in culture, continued to express genes involved in fat, cholesterol, bile acid, and xenobiotic metabolism and had glutathione antioxidant capacity. CYP3A4 activity and urea synthesis by the encapsulated HLC were higher than that of monolayer HLC cultures. Functional HLC can be derived from hAEC, and HLC can be encapsulated within alginate microcapsules without losing viability or function in vitro.

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Section: Properties Of Encapsulated Hepatocyte-like Cells 871mentioning

confidence: 76%

Hepatocyte-Like Cells Derived from Human Amniotic Epithelial Cells Can Be Encapsulated Without Loss of Viability or Function In Vitro

Vaghjiani

Vaithilingam

Saraswati

et al. 2014

Stem Cells and Development

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“…Unfortunately, no consensus exists in the literature on this critical point, so far. Faybik et al [54] , studying IGCPDR using COLD System (Pulsion, Germany) and LiMon (Pulsion, Germany) in a series of patients who underwent OLT found ICGPDR < 10% per minute as a predictor of postoperative complications. Hori et al [55] , using ICGK (Nihon Kohden DDG 2001, Japan) in a cohort of thirty patients admitted to living donor liver transplant, assessed graft function daily for the first 14 postoperative days, and then on POD 21 and 28.…”

Section: Icg Kinetics In Liver Transplant Surgerymentioning

confidence: 99%

Indocyanine green kinetics to assess liver function: Ready for a clinical dynamic assessment in major liver surgery?

Gasperi

Mazza

Prosperi

2016

WJH

145

124

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Indocyanine green (ICG) kinetics (PDR/R15) used to quantitatively assess hepatic function in the perioperative period of major resective surgery and liver transplantation have been the object of an extensive, updated and critical review. New, non invasive bedside monitors (pulse dye densitometry technology) make this opportunity widely available in clinical practice. After having reviewed basic concepts of hepatic clearance, we analysed the most common indications ICG kinetic parameters have nowadays in clinical practice, focusing in particular on the diagnostic and prognostic role of PDR and R15 in the perioperative period of major liver surgery and liver transplantation. As recently pointed out, even if of extreme interest, ICG clearance parameters have still some limitations, to be considered when using these tests. Core tip: Non invasive monitors for indocyanine green (ICG) clearance (PDR and R15) are now available for a rapid assessment of liver function both in the intensive care unit and in major liver surgery. After having reviewed the basic concepts of hepatic clearance, we have analysed the most common indications of ICG kinetic parameters in clinical practice, focusing on the diagnostic and prognostic role of PDR and R15 in the perioperative period of major resective liver surgery and liver transplantation. Since ICG parameters have still some limitations, we will underline the conditions (mainly hyperbilirubinemia and severe peripheral hypoperfusion) able to alter the reliability of these tests.De Gasperi A, Mazza E, Prosperi M. Indocyanine green kinetics to assess liver function: Ready for a clinical dynamic assessment in major liver surgery? World

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“…Die ICGClearance erwies sich bei Lebertransplantation und chronischem Leberversagen als hilfreicher Funktionstest zur Beurteilung der Leberperfusion und als prognos- tischer Faktor bei kritisch kranken Patienten [5]. Eine komplett erloschene Leberdurchblutung wird durch die ICG-PDR korrekt angezeigt [6,7].…”

Section: Messung Des Intraabdominellen Drucksunclassified

Indozyaningrünplasmaverschwinderate

Seibel

Sakka

2010

Anaesthesist

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Increased intraabdominal pressure (IAP) and abdominal compartment syndrome (ACS) are diseases which are often underestimated with respect to incidence and prognosis especially in critically ill patients. The clinical gold standard for the determination of IAP is the urinary bladder measurement technique. For assessment of hepatosplanchnic perfusion the indocyanine green plasma disappearance rate (ICG-PDR) has recently become a clinically attractive method. In this investigation a decrease in splanchnic perfusion caused by increased IAP was observed in critically ill patients with abdominal focused sepsis or postoperative systemic inflammatory response syndrome (SIRS). It was found that the reduction of ICG-PDR as a measure of splanchnic blood flow correlated with the increase of IAP, i.e. increased IAP is associated with lower ICG-PDR. Furthermore, the data suggest that a relevant decrease of splanchnic blood flow may appear even during lower IAP than previously assumed.

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Plasma Disappearance Rate of Indocyanine Green in Liver Dysfunction

Cited by 82 publications

References 12 publications

Hepatocyte-Like Cells Derived from Human Amniotic Epithelial Cells Can Be Encapsulated Without Loss of Viability or Function In Vitro

Hepatocyte-Like Cells Derived from Human Amniotic Epithelial Cells Can Be Encapsulated Without Loss of Viability or Function In Vitro

Indocyanine green kinetics to assess liver function: Ready for a clinical dynamic assessment in major liver surgery?

Indozyaningrünplasmaverschwinderate

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