Plasma detection of NO by a catheter

Goto, Masami; Mochizuki, Seiichi

doi:10.1007/s11517-008-0333-z

Cited by 6 publications

(6 citation statements)

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“…They have already demonstrated the validity of this catheter-type NO sensor for in vivo measurement of NO in the coronary circulation, [2][3][4] and in the present study, the investigators report that a high-fat diet resulted in a significant reduction in intra-arterial NO concentrations, but not in the AChinduced coronary flow response. These findings suggest that in the early stages of coronary endothelial dysfunction other endothelium-derived relaxing factors, such as prostacyclin and endothelium-derived hyperpolarizing factors (EDHF), may contribute to the maintenance of coronary flow after injection of ACh.…”

Section: Article P 562supporting

confidence: 65%

New Approaches to the Evaluation of Coronary Endothelial Function Direct Measurement of Intra-Arterial NO Concentration In Vivo

Hamasaki

2009

Circ J

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itric oxide (NO) is released by endothelial cells in response to hemodynamic forces (eg, shear stress, axial strain) and has vasodilator and anti-atherogenic properties. Endothelial dysfunction is associated with diminished bioavailability of NO and poor prognosis in patients with traditional cardiovascular risk factors. Rather than directly measuring NO concentrations, most previous studies have measured vasodilator activity as a proxy, including assessment of flow-mediated dilation (FMD) and response to intracoronary injection of acetylcholine (ACh). Article p 562In this issue of the Journal, Kume et al 1 demonstrate the utility of a catheter-type NO sensor for evaluating the bioavailability of NO and detecting the early stages of endothelial dysfunction in high-fat-diet-induced obese dogs. They have already demonstrated the validity of this catheter-type NO sensor for in vivo measurement of NO in the coronary circulation, 2-4 and in the present study, the investigators report that a high-fat diet resulted in a significant reduction in intra-arterial NO concentrations, but not in the AChinduced coronary flow response. These findings suggest that in the early stages of coronary endothelial dysfunction other endothelium-derived relaxing factors, such as prostacyclin and endothelium-derived hyperpolarizing factors (EDHF), may contribute to the maintenance of coronary flow after injection of ACh. Furthermore, Ach-induced coronary flow may not be a good proxy for reduced NO bioavailability and coronary endothelial dysfunction.The authors also demonstrated that while fasting plasma insulin levels increased, fasting plasma glucose levels remained constant in their dog model of obesity, which indicates that insulin resistance accompanied the decrease in NO production. Cardillo et al suggested that insulin stimulates both endothelin and NO activity in the skeletal muscle circulation and that an imbalance between the release of these 2 substances may be involved in the pathophysiology of atherosclerosis in insulin-resistant states associated with endothelial dysfunction. 5 In fact, insulin-mediated production of endothelin may increase in the context of insulin resistance and thereby induce the vasoconstriction associated with endothelial dysfunction. 6 Therefore, endothelin may affect vascular tone after injection of ACh in patients with insulin resistance through direct or indirect effects, which may complicate interpretation of the significance of AChinduced vasomotion.Assessment of vasomotion after intracoronary injection of ACh or by FMD is commonly used to evaluate coronary endothelial function. However, evaluation of vascular endothelial function based on these 2 methods depends on the normal function of vascular smooth muscle. If both the basal tone and the response of vascular smooth muscle during the procedure are impaired, the utility of this measure is poor. Furthermore, the data are influenced by multiple endothelium-derived vasodilators other than NO and by vasoconstrictors and neural control of the vascul...

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Section: Article P 562supporting

confidence: 65%

New Approaches to the Evaluation of Coronary Endothelial Function Direct Measurement of Intra-Arterial NO Concentration In Vivo

Hamasaki

2009

Circ J

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“…Recently, we developed a catheter-type NO sensor and demonstrated its validity for in-vivo measurement of NO in the coronary circulation. [24][25][26][27] The purpose of this study was to evaluate the influence of a high-fat diet on the bioavailability of NO by using the catheter-type NO sensor in vivo. …”

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confidence: 99%

Evaluation of Coronary Endothelial Function by Catheter-Type NO Sensor in High-Fat-Diet-Induced Obese Dogs

Kume

Kawamoto

Okura

et al. 2009

Circ J

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Circ J 2009; 73: 562 -567 n the coronary circulation, endothelium-derived nitric oxide (NO) plays a fundamental role in the regulation of blood flow, exerting a tonic vasodilator influence at rest and with increasing myocardial metabolism. 1 The bioavailability of NO is thought to be diminished in subjects with various coronary risk factors and/or pathologic conditions, such as aging, gender, obesity, hypertension, hypercholesterolemia, diabetes mellitus, and metabolic syndrome. [2][3][4][5][6][7][8][9][10][11][12] Thus, endothelial dysfunction, characterized by decreased bioavailability of NO, has been recognized as a predictor of cardiovascular risk and outcome. [13][14][15][16][17] Editorial p 434Coronary endothelial function is most commonly assessed by intracoronary injection of acetylcholine (ACh), which stimulates release of NO and dilates the coronary arteries, but in patients with coronary endothelial dysfunction, infusion of ACh results in paradoxical vasoconstriction. [18][19][20][21][22] This method is widely used in diagnosing coronary vasospastic angina. 17,23 However, there are still many uncertainties about the bioavailability of NO and the precise dynamics of NO have not been investigated. In order to evaluate both endothelial function and the bioavailability of NO in the coronary circulation, direct measurement of the NO concentration in the coronary circulation would be of great value. Recently, we developed a catheter-type NO sensor and demonstrated its validity for in-vivo measurement of NO in the coronary circulation. [24][25][26][27] The purpose of this study was to evaluate the influence of a high-fat diet on the bioavailability of NO by using the catheter-type NO sensor in vivo. MethodsThis study conformed to the Guideline for Care and Use of Laboratory Animals published by the National Institutes of Health (NIH publication No. 85-23, revised 1985) and was conducted under protocols approved by the Kawasaki Medical School Animal Care and Use Committee. High-Fat DietMongrel dogs (n=8) were assigned to either a control group (n=3) that received a regular diet (60 kcal · kg -1 · day -1 ) for 8 months or a high-fat-diet group (n=5; MANINE i/d dryTM, Hill's Nutrition Diet Inc, USA: 60 kcal · kg -1 · day -1 + lard 60 kcal · kg -1 · day -1 ) for 8 months. 28 Catheter-Type NO SensorThe NO sensor (amiNO-700 XL, Innovative Instruments, Tampa, FL, USA; 700 μm in diameter at the tip) was mounted in a 4Fr catheter (1,200 mm long; Hirakawa Hewtech, Tokyo, Japan) and fixed with silicon adhesive. The oxidative current of NO was monitored continuously. (Received August 24, 2008; revised manuscript received October 20, 2008; accepted November 5, 2008; released online February 3, 2009 (n=5) were fed a high-fat diet (120 kcal · kg -1 · day -1 ) for 8 months, then endothelial function was assessed by the change in NO concentration induced by acetylcholine (ACh) (∆NO). Simultaneously, average peak velocity (APV) was obtained by Doppler guide wire. Although fasting plasma glucose levels did not change a...

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“… 8 – 10 The NO sensor showed high sensitivity and high selectivity to NO compared with various NO-related reagents. 9 , 10 We also showed more recently that the chronic application of angiotensin II reduces the plasma NO concentration induced by acetylcholine (ACh). 11 Furthermore, the NO sensor enabled us to record accurate changes in plasma NO levels as a result of release from the endothelium in response to ACh infusion.…”

Section: Introductionmentioning

confidence: 98%

First evaluation of real-time nitric oxide changes in the coronary circulation in patients with non-ischaemic dilated cardiomyopathy using a catheter-type sensor

Takarada

Imanishi

Goto

et al. 2010

European Heart Journal

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AimsNo direct method has yet been developed to measure real-time plasma nitric oxide (NO) concentration in humans. In this study, we evaluated a new method for measuring plasma NO concentration in patients with dilated cardiomyopathy (DCM) and in normal controls using a catheter-type sensor.Methods and resultsWe simultaneously measured average peak velocity (APV) of the coronary artery flow and change in plasma NO concentration using the NO sensor placed in the great cardiac vein of 10 DCM patients and 10 control subjects. These evaluations were performed in response to sequential intracoronary infusions of acetylcholine (ACh, 10−8–10−6 M), NG-monomethyl-l-arginine (l-NMMA, 200 µmol) and co-infusion of ACh and l-NMMA. The change in plasma NO concentration in DCM patients was significantly impaired compared with the control group (P < 0.01). Pretreatment with l-NMMA completely suppressed the ACh-induced NO concentration, whereas APV in the left anterior descending coronary artery was partially suppressed in both groups. Plasma NO concentration reached its peak value later than the maximum APV following the injection of ACh (10−6 M) in both groups.ConclusionThe catheter-type NO sensor could be applied to clinically evaluate the endothelial function (i.e. reduced endothelium-derived NO bioavailability) in patients with cardiovascular diseases.

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Plasma detection of NO by a catheter

Cited by 6 publications

References 82 publications

New Approaches to the Evaluation of Coronary Endothelial Function Direct Measurement of Intra-Arterial NO Concentration In Vivo

New Approaches to the Evaluation of Coronary Endothelial Function Direct Measurement of Intra-Arterial NO Concentration In Vivo

Evaluation of Coronary Endothelial Function by Catheter-Type NO Sensor in High-Fat-Diet-Induced Obese Dogs

First evaluation of real-time nitric oxide changes in the coronary circulation in patients with non-ischaemic dilated cardiomyopathy using a catheter-type sensor

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