Plasma cytokines and chemokines differentiate between active disease and non-active tuberculosis infection

Mihret, Adane; Bekele, Yonas; Bobosha, Kidest; Kidd, Martin; Aseffa, Abraham; Howe, Rawleigh; Walzl, Gerhard

doi:10.1016/j.jinf.2012.11.005

Cited by 90 publications

(75 citation statements)

References 21 publications

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“…However, our findings did not reveal any significant differences in the expression levels of these factors in TB-infected children compared to those in control children. Since VEGF in particular has been shown to be a useful biomarker in adult TB (36,37), our findings suggest that more studies are needed on VEGF expression patterns before the utility of VEGF can be universally applied.…”

Section: Figmentioning

confidence: 93%

Circulating Biomarkers of Pulmonary and Extrapulmonary Tuberculosis in Children

Kumar

Ray

Andrade

et al. 2013

Clin. Vaccine Immunol.

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d Tuberculosis (TB) in children is not only more likely to cause more severe disease than that seen in adults, it is also more likely to be extrapulmonary. Moreover, pediatric TB is very difficult to diagnose and suffers from a lack of understanding of host biomarkers for monitoring the progression of disease. Hence, we sought to identify the expression patterns of a variety of biomarkers in the plasma of children with pulmonary TB (PTB) and extrapulmonary TB (ETB), as well as in healthy control (HC) children. Thus, we examined a variety of circulating markers reflecting tissue inflammation, oxidative stress, innate immune activation, fibrosis, and the cytokine response. Children with active TB, compared to HC children, showed markedly elevated plasma levels of matrix metalloproteinases and their endogenous inhibitors. In addition, children with active TB had significantly elevated levels of C-reactive protein, ␣-2 macroglobulin, and haptoglobin, as well as hemoxygenase 1.

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Section: Figmentioning

confidence: 93%

Circulating Biomarkers of Pulmonary and Extrapulmonary Tuberculosis in Children

Kumar

Ray

Andrade

et al. 2013

Clin. Vaccine Immunol.

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“…Multicytokine signatures in serum have indeed been found to be useful for the differentiation between latent and active TB. Plasma concentrations of EGF, fractalkine, IFN-g, IL-4, MCP-3 (monocyte chemoattractant protein-3), and IP-10 were found to be significantly different between TB patients and healthy household contacts (Mihret et al 2013). An earlier study showed that serum levels of proinflammatory cytokines IL-6 and IP-10 were significantly lower in latently infected individuals than in TB patients, whereas MCP-1 levels were higher (Djoba Siawaya et al 2009a).…”

Section: Biomarkers In Serum and Plasmamentioning

confidence: 94%

Clinical Immunology and Multiplex Biomarkers of Human Tuberculosis

Walzl

Haks

Joosten

et al. 2014

Cold Spring Harbor Perspectives in Medicine

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“…Immune responses to M. tuberculosis infection are downregulated by the production of anti-inflammatory cytokines such as IL-4, IL-10, and transforming growth factor ␤ (TGF-␤) (12). In TB patients, patterns of cytokines and chemokines detected in the blood circulation can provide evidence of infection and/or disease without direct analysis of tissue from the affected organ(s) (e.g., lung biopsy) (13,14).…”

Section: T Uberculosis (Tb) Is Increasingly Viewed As An Imbalance Ofmentioning

confidence: 99%

Exploratory Study on Plasma Immunomodulator and Antibody Profiles in Tuberculosis Patients

Ravindran

Krishnan

Khanum

et al. 2013

Clin Vaccine Immunol

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Host immune responses to Mycobacterium tuberculosis are generally able to contain infection and maintain a delicate balance between protection and immunopathology. A shift in this balance appears to underlie active disease observed in about 10% of infected individuals. Effects of local inflammation, combined with anti-M. tuberculosis systemic immune responses, are directly detectable in peripheral circulation, without ex vivo stimulation of blood cells or biopsy of the affected organs. We studied plasma immunomodulator and antibody biomarkers in patients with active pulmonary tuberculosis (TB) by a combination of multiplex microbead immunoassays and computational tools for data analysis. Plasma profiles of 10 immunomodulators and antibodies against eight M. tuberculosis antigens (previously reported by us) were examined in active pulmonary TB patients in a country where TB is endemic, Pakistan. Multiplex analyses were performed on samples from apparently healthy individuals without active TB from the same community as the TB patients to establish the assay baselines for all analytes. Over 3,000 data points were collected from patients (n ‫؍‬ 135) and controls (n ‫؍‬ 37). The data were analyzed by multivariate and computer-assisted cluster analyses to reveal patterns of plasma immunomodulators and antibodies. This study shows plasma profiles that in most patients represented either strong antibody or strong immunomodulator biomarkers. Profiling of a combination of both immunomodulators and antibodies described here may be valuable for the analysis of host immune responses in active TB in countries where the disease is endemic.

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Plasma cytokines and chemokines differentiate between active disease and non-active tuberculosis infection

Cited by 90 publications

References 21 publications

Circulating Biomarkers of Pulmonary and Extrapulmonary Tuberculosis in Children

Circulating Biomarkers of Pulmonary and Extrapulmonary Tuberculosis in Children

Clinical Immunology and Multiplex Biomarkers of Human Tuberculosis

Exploratory Study on Plasma Immunomodulator and Antibody Profiles in Tuberculosis Patients

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