Plasma cortisol variations induced in the stallion by mating

Tamanini, Carlo; Giordano, N.; Chiesa, F.; Seren, E.

doi:10.1530/acta.0.1020447

Cited by 55 publications

(35 citation statements)

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“…Hormone analysis Cortisol plasma content was determined by a validated radioimmunoassay (Tamanini et al, 1983). Cortisol, H 3 -labelled cortisol and anti-cortisol antiserum were purchased from Sigma-Aldrich (St Louis, MO, USA).…”

Section: Measurementsmentioning

confidence: 99%

Effects of dietary nucleotide supplementation on growth performance and hormonal and immune responses of piglets

Superchi

Saleri

Borghetti

et al. 2012

Animal

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The effects of dietary nucleotide supplementation from 9 days of age until the end of post-weaning on piglets hormonal and immune responses and on growth performance were investigated. During lactation (days 9 to 21) and post-weaning (days 22 to 55) 10 [HBI Fomeva11 3 (Large White 3 Landrace)] litters (n 5 108 piglets) had ad libitum access to two standard diets, both supplemented with 0% (T0 group) or 0.1% (T1 group) of yeast extract nucleotides. BW of piglets at days 21 (P , 0.10), 35 and 55 (P , 0.05) was greater in T1 compared with T0. Feed intake was not different between groups (P . 0.05). Cortisol content was lower in T1 than in T0 at days 28 and 35 (P , 0.05), whereas growth hormone was lower at day 35 (P , 0.05). Levels of IGF-1 were similar across groups (P . 0.05). Nucleotide-supplemented diets increased lymphocyte subpopulation CD42CD81 high at days 21 and 35 (P , 0.05), whereas CD41CD82 cells were higher in T1 than in T0 at day 21 (P , 0.05). Peripheral blood mononuclear cells cytokine expression was influenced by dietary nucleotide supplementation. At weaning, interleukin (IL)-6 and IL-1b expression was lower (P , 0.05) in T1 compared with T0, whereas the expression of interferon (IFN)-g and IL-10 was higher (P , 0.05). At day 28, piglets in T1 showed higher values of tumor necrosis factor (TNF)-a expression than T0 and lower values of IL-10 expression (P , 0.05). Dietary nucleotide supplementation had a suppressive effect on IL-6 and IL-10 expression (P , 0.05) at day 35. On the contrary, the expression of IFN-g, TNF-a and IL-1b was enhanced (P , 0.05). In conclusion, these results suggest that starting a dietary nucleotide supplementation before weaning can improve the adaptive capabilities of weaned piglets to the stressors, enhancing the growth performance.

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Section: Measurementsmentioning

confidence: 99%

Effects of dietary nucleotide supplementation on growth performance and hormonal and immune responses of piglets

Superchi

Saleri

Borghetti

et al. 2012

Animal

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“…The within-assay coefficient of variation (WACV) and the between-assay coefficient of variation (BACV) were 9.7% and 13.8%, respectively. Plasma levels of cortisol were determined as reported by Tamanini et al (1983) using a RIA method. The sensitivity (90% B/B 0 ) of the cortisol antibody was 4.9 ng/ml, and the cross-reactivities were as follows: 20.4% with cortisone, 74.6% with deoxycortisol-11a, 1.1% with corticosterone and 0% with progesterone and oestrogens.…”

Section: Shearing Stress and Hormonal Responsementioning

confidence: 99%

The effect of shearing procedures on blood levels of growth hormone, cortisol and other stress haematochemical parameters in Sarda sheep

Carcangiu

Vacca

Parmeggiani

et al. 2008

Animal

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The aim of this research was to investigate how growth hormone (GH) cortisol and some haematochemical parameters could be modified by the stress caused by the stages of shearing in Sarda breed sheep. Five groups of 10 sheep each were formed. Group A, only separated from the flock; Group B, only tied; Group C, both tied and shorn (animals in these three groups were ewe lambs shorn for the first time); Group D, adult females both tied and shorn; and Group E, adult entire males both tied and shorn (animals in these two groups had been shorn previously). Five blood samples were taken from each animal: the day before treatment (first sample); at the start of the treatment (second sample); in the middle of shearing for Groups C, D and E, 10 min after separation in Group A and 10 min after tying in Group B (third sample); at the end of treatment (fourth sample); and on the day after treatment (fifth sample). Plasma GH levels showed a decrease (P , 0.01) in Groups A, B, C and D during treatment (third and fourth samples), while Group E only at the end of shearing (fourth sample). In the third sample, the highest GH levels were recorded for Group E (P , 0.05), while it was recorded in the fourth sample for Groups A and E (P , 0.05). Cortisol levels showed a clear increase (P , 0.01) in all groups during treatment, but Group A showed a decrease in the fourth sample in comparison to the third sample. Males in the second, third and fourth sample and Group A only in the fourth sample showed lower cortisol levels when compared with the other groups (P , 0.05). Plasma glucose levels showed an increase (P , 0.01) in all groups during treatment but Groups B, C and E showed the highest values (P , 0.05). Magnesium (Mg) showed an increase in all groups in the third and fourth sample, while sodium (Na), in the same samples, only in Groups B, C and D. Potassium (K) values showed a significant decrease (P , 0.05) only in Groups C and D at the end of shearing. These results show that GH secretion is influenced by all the stress procedure: separation, tying and shearing. Shearing, even if necessary for animals, causes a significant change of the blood parameters involved in the stress response.

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“…8 In equine breeding, the mare is not only exposed to repeated restraint and gynaecological examinations, but also to other stressful events such as transportation to the breeding farm and loss of the normal environment and social companions; all together these events may cause increased secretion of glucocorticoids and cortisol. Exercise, 9-11 transportation, 12 pain, 13 social stress, 14 acute exercise, 15 and sexual activity 16,17 increased cortisol in horses. A potential indicator of animal welfare is the absence of stress, but to date there is no standard definition of stress and no single biochemical assay system to measure stress.…”

Section: Introductionmentioning

confidence: 98%

Stress and its effects on horses reproduction

AboEl-Maaty

2011

Vet Sci Develop

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A total of 90 mares and horses were subjected to blood sampling for determining the effect of management (farm), reproductive condition, sex, age, breed and month of the year during breeding on circulating levels of cortisol and sex hormones. Blood samples were collected from December to the following June from four farms. Blood sera underwent testosterone, estradiol, progesterone and cortisol assaying using ELISA kits. Cortisol levels were significantly low in lactating mares during their foal heat but significantly high levels were recorded in both repeat breeder mares and horses used for racing. High and significant testosterone and estradiol levels were recorded in both stallions used for breeding especially after semen collection and early pregnant mares. Similar testosterone levels were recorded in both early pregnant mares and racing horses but high levels were recorded in stallions. Estradiol was high in both early pregnant and mares with endometritis but the highest levels were observed in stallions. Horses held in private farms had high cortisol levels compared to those of governmental farms. In contrast to mares, horses had low cortisol and high estradiol levels. Cortisol levels were high from April to June (Spring and early summer) compared to its levels from December to March (Winter). Arab horses had low cortisol compared to native and imported foreign breeds. In conclusion, environmental condition, exercise, breed, management and the purpose of raising horses all are affecting its cortisol levels.

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Plasma cortisol variations induced in the stallion by mating

Cited by 55 publications

References 0 publications

Effects of dietary nucleotide supplementation on growth performance and hormonal and immune responses of piglets

Effects of dietary nucleotide supplementation on growth performance and hormonal and immune responses of piglets

The effect of shearing procedures on blood levels of growth hormone, cortisol and other stress haematochemical parameters in Sarda sheep

Stress and its effects on horses reproduction

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