Plasma concentrations of adrenomedullin correlate with the extent of pulmonary hypertension in patients with mitral stenosis

Nishikimi, Toshio; Nagata, Seiki; Sasaki, Tatsuya; Tomimoto, Shigehiro; Matsuoka, Hiroaki; Takishita, Shuichi; Kïtamura, Kazuo; Miyata, Atsuro; Matsuo, Hisayuki; Kangawa, Kenji

doi:10.1136/hrt.78.4.390

Cited by 56 publications

(34 citation statements)

References 44 publications

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“…12 Also, in the clinical setting, plasma AM concentration was increased in patients with pulmonary hypertension associated with mitral stenosis. 13 AM could have a potential organ-protective action, probably by reducing oxidative stress. 14 In fact, AM inhibited ROS generation in mesangial cells, which may be related to the reduction of MAPK-mediated mitogenesis.…”

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confidence: 99%

Adrenomedullin Can Protect Against Pulmonary Vascular Remodeling Induced by Hypoxia

et al. 2004

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Background-Chronic hypoxia is one of the major causes of pulmonary vascular remodeling associated with stimulating reactive oxygen species (ROS) production. Recent studies have indicated that hypoxia upregulates expression of adrenomedullin (AM), which is not only a potent vasodilator but also an antioxidant. Thus, using heterozygous AM-knockout (AM ϩ/Ϫ ) mice, we examined whether AM could attenuate pulmonary vascular damage induced by hypoxia. Methods and Results-Ten-week-old male wild-type (AM ϩ/ϩ ) or AM ϩ/Ϫ mice were housed under 10% oxygen conditions for 3 to 21 days. In AM ϩ/ϩ mice, hypoxia enhanced AM mRNA expression, which was reduced by the administration of a superoxide dismutase mimetic, 4-hydroxy-2,2,6,6-tetramethyl-piperidine-N-oxyl (hydroxy-TEMPO). Hypoxia induced pulmonary vascular remodeling, which was associated with the increased production of oxidative stress measured by electron spin resonance and immunostaining of 3-nitrotyrosine. The media wall thickness of the pulmonary arteries was significantly greater in AM ϩ/Ϫ mice housed under hypoxia than in AM ϩ/ϩ mice under hypoxia. Concomitantly, pulmonary ROS production induced by hypoxia was more enhanced in AM ϩ/Ϫ mice than in AM

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confidence: 99%

Adrenomedullin Can Protect Against Pulmonary Vascular Remodeling Induced by Hypoxia

et al. 2004

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“…1 (25, 27). AM was reacted simultaneously with 2,4-DNP-biotinyl-BSA-anti-AM [12][13][14][15][16][17][18][19][20][21][22][23][24][25] Figure 2 shows a typical calibration curve for rat AM-Gly, AM-m and AM-t. The lower detection limit of AM-Gly was 1 amol/assay; the assay range for plasma was 50 fmol/l (20-µl plasma samples).…”

Section: Immune Complex Transfer Enzyme Immunoassay For Rat Am-glymentioning

confidence: 99%

“…To measure rat AM-m, an aliquot (10 µl) of standard AM-m, plasma sample or tissue extract was mixed with 140 µl of AM buffer containing 0.1% Triton X-100, 50 KIU/ml of aprotinin, 10 fmol of 2,4-DNP-biotinyl-BSA-anti-AM [12][13][14][15][16][17][18][19][20][21][22][23][24][25] Fab conjugate (as a capture antibody) and 3 fmol of anti-AM[46-52]NH2 Fab -β-D-galactosidase conjugate (as an antibody-enzyme) and incubated overnight at 4 ºC without shaking (first incubation; Formation). Thereafter, the mixture was incubated with one streptavidin-coated polystyrene bead for 30 min at room temperature with shaking (210 strokes/min, second incubation; Entrapment).…”

Section: Two-site Immunoenzymometric Assay (Iema) For Rat Am-m and Am-tmentioning

confidence: 99%

“…We used three anti-AM monoclonal antibodies (23,24): one recognized the ring structure (rAdR-2; anti-rat AM [8][9][10][11][12][13][14][15][16][17][18][19][20][21][22][23]), one the C-terminal amide structure (AdC-1; anti-AM[46-52]NH2), and one the middle portion (AdM-2; anti-AM [25][26][27][28][29][30][31][32][33][34][35][36]) of the molecule. These antibodies were gifted from Shionogi & Co., Ltd., Osaka, Japan.…”

Section: Preparation Of Antibodiesmentioning

confidence: 99%

“…Monoclonal mouse anti-AM [12][13][14][15][16][17][18][19][20][21][22][23][24][25]Fab was conjugated with 6-maleimidohexanoyl-2,4 DNP-biotinyl-BSA and used as a capture antibody (26,27 (26,27).…”

Section: Preparation Of Capture Antibody and Antibody-labeled Enzymementioning

confidence: 99%

See 2 more Smart Citations

Direct Measurement of Glycine-Extended Adrenomedullin in Plasma and Tissue Using an Ultrasensitive Immune Complex Transfer Enzyme Immunoassay in Rats

et al. 2003

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Pulmonary Vascular Disease Related to Hemodynamic Stress in the Pulmonary Circulation

Chan

Loscalzo

2010

Comprehensive Physiology

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Hemodynamic stress in the pulmonary vessel is directly linked to the development of vascular remodeling and dysfunction, ultimately leading to pulmonary hypertension. Recently, some advances have been made in our molecular understanding of the exogenous upstream stimuli that initiate hemodynamic pertubations as well as the downstream vasoactive effectors that control these responses. However, much still remains unknown regarding how these complex signaling pathways connect in order to result in these characteristic pathophysiological changes. This chapter will describe our current understanding of and needed areas of research into the clinical, physiological, and molecular changes associated with pressure/volume overload in the pulmonary circulation. © 2011 American Physiological Society. Compr Physiol 1:123‐139, 2011.

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Plasma concentrations of adrenomedullin correlate with the extent of pulmonary hypertension in patients with mitral stenosis

Cited by 56 publications

References 44 publications

Adrenomedullin Can Protect Against Pulmonary Vascular Remodeling Induced by Hypoxia

Adrenomedullin Can Protect Against Pulmonary Vascular Remodeling Induced by Hypoxia

Direct Measurement of Glycine-Extended Adrenomedullin in Plasma and Tissue Using an Ultrasensitive Immune Complex Transfer Enzyme Immunoassay in Rats

Pulmonary Vascular Disease Related to Hemodynamic Stress in the Pulmonary Circulation

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