Plasma concentrations of 13,14-dihydro-15-keto prostaglandin F2-alpha (PGFM), progesterone and estradiol in pregnant and nonpregnant diestrus cross-bred bitches

Luz, Marcelo Rezende; Bertan, Claudia; Binelli, Mário; Lopes, Maria Denise

doi:10.1016/j.theriogenology.2006.01.036

Cited by 44 publications

(30 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Plasma progesterone concentrations were similar in the two Beagle groups and in the two GSD groups, especially during late luteal phase (Beagles: non‐pregnant 5.2 ± 2.2 ng/ml, pregnant 5.2 ± 1.2 ng/ml, GSD: non‐pregnant 2.6 ± 1.4 ng/ml, pregnant 3.8 ± 0.9 ng/ml). This is in contrast to the observations of Luz et al. (2006) who found apparently lower progesterone concentrations in non‐pregnant compared with pregnant bitches.…”

Section: Discussioncontrasting

confidence: 99%

Comparison of Selected Endocrine Parameters During Luteal Phase and Pregnancy in German Shepherd Dogs and Beagles

Günzel‐Apel¹,

Beste²,

Nottorf³

et al. 2009

Reprod Domestic Animals

View full text Add to dashboard Cite

Contents Concentrations of progesterone, prolactin and relaxin in serum at predetermined intervals after ovulation (day 0) in non‐pregnant and pregnant normocyclic Beagles were assayed and results compared with those observed in German Shepherd dogs (GSD) in a previous study. The goal was to determine possible reproductive hormone specificities related to the GSD breed. Furthermore, the effects of medroxyprogesterone acetate (MPA)‐treatment in non‐pregnant Beagles and of progesterone supplementation in pregnant Beagles on the hormone concentrations were examined. Mean concentrations of progesterone and prolactin were not different in the non‐pregnant Beagles compared with those seen in non‐pregnant GSD, except at days 50–60, when progesterone concentrations were found to be higher in Beagles (p < 0.05). Mean progesterone concentrations in pregnant Beagles at days 50–60 after ovulation (day 0) were higher (p < 0.05) than in GSD at that time, but not at earlier time periods. Prolactin concentrations were higher (p < 0.05) in Beagles throughout pregnancy compared with those in the GSD. Mean relaxin concentrations were numerically but not significantly lower in GSD than in Beagles throughout pregnancy. A 10‐day oral MPA treatment did not affect progesterone or prolactin secretion in normocyclic non‐pregnant Beagles. Medroxyprogesterone acetate serum concentrations were approximately 3.9 ng/ml during treatment and decreased to 0.42 and 0.021 ng/ml within 5 and 15 days after end of treatment, respectively. Intramuscular progesterone supplementation from days 30 to 40 in pregnant Beagles resulted in higher concentrations of progesterone in the 36‐ to 45‐day time periods; prolactin and relaxin concentrations were not significantly affected during or after treatment compared with administration of placebo. The results suggest a tendency towards deficient luteal function in the short‐cycle GSD bitches previously studied, which in pregnancy may reflect the observed decreased prolactin concentrations; the possibility that GSD relaxin secretion is deficiency required needs further study. As oral treatment with MPA did not affect progesterone and prolactin release, it may be useful for studying luteal function in pregnant bitches with suspected hypoluteoidism.

show abstract

Section: Discussioncontrasting

confidence: 99%

Comparison of Selected Endocrine Parameters During Luteal Phase and Pregnancy in German Shepherd Dogs and Beagles

Günzel‐Apel¹,

Beste²,

Nottorf³

et al. 2009

Reprod Domestic Animals

View full text Add to dashboard Cite

show abstract

“…Our initial expectation was that PGFM might be a suitable pregnancy indicator in all carnivores. However, in most non-felid carnivore species investigated, we were able to detect a sharp PGFM peak before parturition only, indicating the luteolytic function of PGF 2␣ as described in ruminants [30] and in bitches [31]. Further investigation into PGFM production in other carnivores is warranted.…”

Section: Discussionmentioning

confidence: 63%

“…It is now widely accepted that uterine and placental prostaglandins play a key role in regulating the function and life span of corpora lutea [16] and exogenous PGF␣ is luteolytic in both pregnant and pseudo-pregnant bitches [17]. Serum PGFM analyses in the dog revealed different patterns between pregnant and non-pregnant (diestrus) bitches [18].…”

Section: Introductionmentioning

confidence: 99%

Using PGFM (13,14-dihydro-15-keto-prostaglandin F2α) as a non-invasive pregnancy marker for felids

et al. 2012

View full text Add to dashboard Cite

“…According to Tsutsui et al (2001a), the endometrial hypertrophy, accentuated by a progesteronic action, makes the infusion of uterine flushing medium difficult when the OHE is not performed. Thus, it is possible that this uterine condition, more or less pronounced, caused by individual sensibility or even variations on the concentration of plasmatic progesterone (Luz et al 2006) influence the embryonic collection also after the OHE, preventing or making a constancy difficult in the repeatability of the embryonic recovery rates. Another possibility for the lack of embryos in these animal's flushings is the presence of the embryos in the uterine tubes, instead of the uterine horns.…”

Section: Discussionmentioning

confidence: 99%

High Embryonic Recovery Rates with In vivo and Ex vivo Techniques in the Bitch

Luz¹,

Holanda²,

Pereira³

et al. 2010

Reprod Domestic Animals

View full text Add to dashboard Cite

The embryonic collection techniques in dogs present a vast methodological variation and low recovery rates. The objectives were to compare and describe two techniques as to the recovery of canine embryos, on the 12th day after the first mating or artificial insemination. Embryos were recovered through uterine horn flushing in vivo, before performing the ovariohysterectomy (OHE) (Group 1; n = 9) or ex vivo, immediately after the OHE (Group 2; n = 9). In total, 43 and 47 embryonic structures were recovered in Groups 1 and 2, respectively. There was no significant difference (p > 0.05) between groups on recovery rates (72.8% and 81.0%, respectively). We inferred that both in vivo and ex vivo techniques allow a high rate of embryonic recovery; in the collection technique prior to the OHE, it is essential to carefully handle the reproductive system during the trans-surgical period and that the 12th day (D12) after the first mating/artificial insemination is an efficient option for the high recovery rate of morulae and blastocysts.

show abstract

Plasma concentrations of 13,14-dihydro-15-keto prostaglandin F2-alpha (PGFM), progesterone and estradiol in pregnant and nonpregnant diestrus cross-bred bitches

Cited by 44 publications

References 14 publications

Comparison of Selected Endocrine Parameters During Luteal Phase and Pregnancy in German Shepherd Dogs and Beagles

Comparison of Selected Endocrine Parameters During Luteal Phase and Pregnancy in German Shepherd Dogs and Beagles

Using PGFM (13,14-dihydro-15-keto-prostaglandin F2α) as a non-invasive pregnancy marker for felids

High Embryonic Recovery Rates with In vivo and Ex vivo Techniques in the Bitch

Contact Info

Product

Resources

About