Plasma concentrations of 13,14-dihydro-15-keto-prostaglandin F during pregnancy in sheep

Mitchell, Murray D.; Flint, A. P. F.; Turnbull, A. C.

doi:10.1016/0090-6980(76)90154-4

Cited by 61 publications

(41 citation statements)

References 9 publications

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“…Plasma samples were aspirated and stored at À208C until analysis. Plasma concentrations of 13,14-dihydro-15-ketoprostaglandin F 2a (PGFM) were measured using a radioimmunoassay (Mitchell et al 1976). The inter-and intra-assay coefficients of variation were 20.4 and 7.2% (high), 24.1 and 10.3% (medium) and 28.1 and 16.4% (low), respectively.…”

Section: Oxytocin Challengementioning

confidence: 99%

Evaluation of the uterine environment early in pregnancy establishment to characterise cows with a potentially superior ability to support conceptus survival

Ledgard

Meier

Peterson

2011

Reprod. Fertil. Dev.

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Abstract. During previous investigations, the capacity of the cow to secrete prostaglandin in response to oxytocin has been linked with pregnancy outcome. The objective of the present study was to evaluate the predictive value of prostaglandin release to identify groups of cows as potentially superior (SR, low prostaglandin release) or inferior (IR, high prostaglandin release) for pregnancy outcome and to utilise these cows to investigate factors that contribute to optimum uterine conditions for early pregnancy. Animals were synchronised and received an in vitro-derived blastocyst on Day 7 post-oestrus. Tissues (trophoblast and endometrial) and uterine luminal fluid (ULF) were recovered 10 days later. Pregnancy rates were 94 and 78% for SR and IR cows, respectively. Of the pregnant SR cows, 69% had larged conceptuses (.24 cm) in contrast to 43% IR of cows. IR cows with small conceptuses (,12 cm) had significantly lower mean Day 3 and 5 post-oestrous progesterone concentrations than cows with large conceptuses. The expression of factors involved in the prostaglandin pathway, pregnancy and conceptus development were analysed via quantitative RT-PCR and Western blot analysis. Investigation of 16 endometrial gene transcripts indicated no differences between IR and SR cows except for osteopontin expression which, in uteri with large conceptuses, was 2-fold greater in SR than IR cows (P ¼ 0.02). There was greater expression of CTGF, OXTR, PGES, PGHS2 and UTMP mRNA in uteri of SR and IR cows that had large compared with small conceptuses (P , 0.05). More IFNT protein was recovered in SR compared with IR ULF (P , 0.03). SR cows with large conceptuses had less TIMP2 and legumain protein in their gravid, compared with their non-gravid horns (P # 0.02) whereas IR cows did not. The predictive value of prostaglandin release in response to oxytocin challenge does not appear to be an effective indicator of subsequent pregnancy rates in cows. Differences between the two groups appear to be associated with subtle differences in progesterone and uterine protein concentrations that may be related to differences in conceptus size.

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Section: Oxytocin Challengementioning

confidence: 99%

Evaluation of the uterine environment early in pregnancy establishment to characterise cows with a potentially superior ability to support conceptus survival

Ledgard

Meier

Peterson

2011

Reprod. Fertil. Dev.

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“…Several studies have shown clearly that prostaglandins (PG) increase in ovine maternal and fetal plasma and amniotic fluid during normal parturition (Currie, Wong, Cox & Thorburn, 1973 ; Flint. Anderson, Patten & Turnbull, 1974;Challis, Dilley, Robinson & Thorburn, 1976;Mitchell, Ellwood, Anderson & Turnbull, 1978;Rawlings & Ward, 1978) dexamethasone-induced pre-term labour and parturition (Liggins & Grieves, 1971;Thorburn, Nicol, Bassett, Shutt & Cox, 1972;Currie et ai, 1973;Mitchell, Flint & Turnbull, 1976;Kendalls al., 1977;Mitchell et al, 1978). Prostaglandin F-2a is known to stimulate myometrial contractile activity in sheep and promote pre-term labour (Liggins, Fairclough, Grieves, Kendall & Knox, 1973;Mitchell et ai, 1977a), while suppression of PG biosynthesis delays the onset of dexamethasone-or ACTH-induced pre-term delivery (Mitchell & Flint, 1978a; Evans, Kennedy, Patrick & Challis, 1982b).…”

Section: Introductionmentioning

confidence: 99%

Early changes in prostaglandin concentrations in ovine maternal and fetal plasma, amniotic fluid and from dispersed cells of intrauterine tissues before the onset of ACTH-induced pre-term labour

Olson¹,

Lye²,

Skinner³

et al. 1984

Reproduction

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“…The circulating concentration of 13,14 dihydro-15-ketoprostaglandin F2Cz (PGFM), the stable metabolite of prostaglandin F2a, has been used as an index of uterine prostaglandin F2oz (PGF2a) production during pregnancy in a variety of species including the sheep (Green, Bygdeman, Toppozada & Wiquist, 1974;Fairclough & Rayne, 1975;Mitchell, Flint & Turnbull, 1976;Mitchell, Clover, Thorburn & Robinson, 1978;Gemmill, Jenkin & Thorburn, 1980). In vitro studies have demonstrated that the uterine tissues contain the enzymes necessary for PGFM production and can metabolize PGF2a during pregnancy (Keirse, Hicks & Turnbull, 1976;Keirse, Mitchell & Flint, 1977).…”

Section: Introductionmentioning

confidence: 99%

The Effect of the Nutritional State on Uterine Prostaglandin F Metabolite Concentrations in the Pregnant Ewe During Late Gestation

Fowden

Silver

1983

Exp Physiol

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SUMMARYThe effects of nutritional state and nutrient availability on arterial and uterine venous concentrations of 13, 14 dihydro-15-ketoprostaglandin F2a (PGFM) have been investigated in chronically catheterized pregnant ewes during late gestation (120-144 d; term = 147 d). Before the daily feed of concentrates was given, a significant positive venous-arterial (V-A) difference in plasma PGFM concentration was observed across the uterus, irrespective of the number of fetuses or the gestational age (mean value at 135 + 2 d: 0-42 + 006 ng/ml, n = 13, P < 0-01). However, by 3-5 h after feeding, no significant difference in PGFM concentration could be detected across the uterus. Withdrawal of food but not water for 48 h increased the plasma PGFM levels and widened the V-A difference in PGFM concentration across the uterus at all gestational ages studied but had the greatest effect in late gestation (> 137 d). These changes in plasma PGFM were accompanied by a fall in glucose and a rise in free fatty acid (FFA) concentration in the arterial plasma. When these metabolite changes were reversed at the end of the fast either by refeeding or by glucose infusion, there was a rapid fall in PGFM concentration and a narrowing of the uterine V-A difference. Induction of hypoglycaemia by infusion of insulin also led to a widening of the V-A difference in PGFM concentration across the uterus. There was a significant inverse correlation between the arterial plasma glucose level and the V-A difference in PGFM concentration across the uterus during feeding, fasting and insulin infusion. The V-A difference in PGFM concentration was also positively correlated with the arterial plasma FFA level under these conditions. When arterial plasma concentrations of PGFM rose above 3 ng/ml, during fasting or insulin induced hypoglycaemia, the ewes lambed within 12-24 h of restoring the metabolite concentrations. These observations indicate that the output of PGFM by the uterus is controlled in some way by the availability of nutrients and emphasize the importance of a regular food intake during late gestation in the ewes.

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Plasma concentrations of 13,14-dihydro-15-keto-prostaglandin F during pregnancy in sheep

Cited by 61 publications

References 9 publications

Evaluation of the uterine environment early in pregnancy establishment to characterise cows with a potentially superior ability to support conceptus survival

Evaluation of the uterine environment early in pregnancy establishment to characterise cows with a potentially superior ability to support conceptus survival

Early changes in prostaglandin concentrations in ovine maternal and fetal plasma, amniotic fluid and from dispersed cells of intrauterine tissues before the onset of ACTH-induced pre-term labour

The Effect of the Nutritional State on Uterine Prostaglandin F Metabolite Concentrations in the Pregnant Ewe During Late Gestation

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