Plasma concentration of selected biochemical markers of endothelial dysfunction in women with various severity of chronic venous insufficiency (CVI)—A pilot study

Budzyń, Magdalena; Iskra, Maria; Turkiewicz, Wojciech; Krasiński, Zbigniew; Gryszczyńska, Bogna; Kasprzak, Magdalena Paulina

doi:10.1371/journal.pone.0191902

Cited by 15 publications

(6 citation statements)

References 68 publications

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“…[20][21][22][23] Other inflammation-related markers, such as interleukin-6, ICAM-1 (intercellular adhesion molecule-1), VCAM1 (vascular cell adhesion molecule-1), E-selectin, P-selectin, and VWF (von Willebrand factor) have been used as markers for EC dysfunction. [23][24][25][26] Plasma levels of these markers can be increased in response to CVD risk factor exposure, [27][28][29] and higher E-and P-selectin levels were associated with impaired acetylcholine-dependent EC vasodilation in humans, 30 indicating that they can be linked to vascular changes beyond the inflammatory response. It remains unclear whether EC dysfunction is synonymous with, or limited to, inhibited vasoreactivity or inflammation-induced activation.…”

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confidence: 99%

Identification of Endothelial Proteins in Plasma Associated With Cardiovascular Risk Factors

Iglesias

Kruse

Sánchez-Rivera

et al. 2021

ATVB

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Objective: Endothelial cell (EC) dysfunction is a well-established response to cardiovascular disease risk factors, such as smoking and obesity. Risk factor exposure can modify EC signaling and behavior, leading to arterial and venous disease development. Here, we aimed to identify biomarker panels for the assessment of EC dysfunction, which could be useful for risk stratification or to monitor treatment response. Approach and Results: We used affinity proteomics to identify EC proteins circulating in plasma that were associated with cardiovascular disease risk factor exposure. Two hundred sixteen proteins, which we previously predicted to be EC-enriched across vascular beds, were measured in plasma samples (N=1005) from the population-based SCAPIS (Swedish Cardiopulmonary Bioimage Study) pilot. Thirty-eight of these proteins were associated with body mass index, total cholesterol, low-density lipoprotein, smoking, hypertension, or diabetes. Sex-specific analysis revealed that associations predominantly observed in female- or male-only samples were most frequently with the risk factors body mass index, or total cholesterol and smoking, respectively. We show a relationship between individual cardiovascular disease risk, calculated with the Framingham risk score, and the corresponding biomarker profiles. Conclusions: EC proteins in plasma could reflect vascular health status.

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confidence: 99%

Identification of Endothelial Proteins in Plasma Associated With Cardiovascular Risk Factors

Iglesias

Kruse

Sánchez-Rivera

et al. 2021

ATVB

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“…Although endothelial dysfunction is considered to be implicated in the pathogenesis of CVI, the number of studies assessing the endothelial status in patients with venous disease is limited. Budzyń et al measured the concentration of selected markers of endothelial dysfunction: von Willebrand factor (vWf), soluble P selectin (sPselectin), soluble thrombomodulin (sTM) and soluble VE cadherin (sVE-cadherin) in CVI women who constitute the most numerous group of patients suffering from venous disease (19). They showed the presence of endothelial dysfunction in patients with CVI and thought that it may be associated with inflammation and enhanced oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

Higher serum Endocan levels are involved in the pathophysiology of chronic venous insufficiency

Doğduş

Koç

2020

Ege Tıp Dergisi

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Aim: Chronic venous insufficiency (CVI) is a common but neglected pathology of the cardiovascular system with high diagnosis and treatment costs and negative effects on patients' quality of life. Endocan is a dermatan sulfate proteoglycan and secreted by activated vascular endothelium. We hypothesized that higher Endocan levels may be associated with the pathophysiology of CVI. Thus, in the current study, we aimed to assess the relationship between serum Endocan levels and CVI. Materials and Methods: Forty-four patients with CVI and 50 age-and gender-matched subjects were enrolled into the study. The baseline clinical characteristics of the patients were obtained and serum Endocan levels were calculated. Results: The mean Endocan level and mean triglyceride (TG) level were significantly higher in the CVI (+) group compared to the CVI (-) group (p<0.001 and p=0.001, respectively). In multivariate logistic regression analysis; Endocan (p<0.001, Odds ratio (OR) = 3.48, 95% Confidence interval (C.I.) = 1.54-8.16), and TG (p=0.009, OR = 1.85, 95% C.I. = 1.36-3.55) were found to be independent predictors of CVI. ROC analysis was performed to find out the ideal Endocan cut-off value for predicting CVI. An Endocan value of >2.58 ng/mL has 92.4% sensitivity, 76.6% specificity for the prediction of the CVI (AUC 0.841, (p<0.001)). Conclusions: In the present study, we evaluated the relationship between serum Endocan levels and CVI. Our findings suggest that increased Endocan levels may be involved in the pathogenesis of CVI.

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“…Nevertheless, cellular senescence could be involved in the pathophysiology of the chronic venous disease as it is generally associated with age-related disorders. 40 Inflammation and endothelial dysfunction are relevant too 41 as for example hsCRP (high sensitive c-reactive protein), vWF (von Willebrand factor), or D-Dimer blood values seem to be higher in varicose veins. 42 Especially endothelial senescence and its relation to vascular endothelial dysfunction could play a key role.…”

Section: Limitationsmentioning

confidence: 99%

Cellular senescence at the saphenofemoral junction in patients with healthy, primary varicose and recurrent varicose veins – A pilot study

et al. 2021

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Objectives Cellular senescence could play a role in the development of venous disease. Superficial venous reflux at the saphenofemoral junction is a common finding in patients with primary varicose veins. Furthermore, reflux in this essential area is associated with higher clinical stages of the disease and recurrent varicose veins. Therefore, this pilot study aimed to investigate cellular senescence in the immediate area of the saphenofemoral junction in patients with healthy veins, primary varicose veins and additionally in patients with recurrent varicose veins due to a left venous stump. Methods We analyzed vein specimens of the great saphenous vein immediately at the saphenofemoral junction. Healthy veins were collected from patients who underwent arterial bypass reconstructions. Samples with superficial venous reflux derived from patients who received high ligation and stripping or redo-surgery at the groin, respectively. Sections were stained for p53, p21, and p16 as markers for cellular senescence and Ki67 as a proliferation marker. Results A total of 30 samples were examined (10 healthy, 10 primary varicose, and 10 recurrent varicose veins). We detected 2.10% p53+ nuclei in the healthy vein group, 3.12% in the primary varicose vein group and 1.53% in the recurrent varicose vein group, respectively. These differences were statistically significant ( p = 0.021). In the healthy vein group, we found 0.43% p16+ nuclei. In the primary varicose vein group, we found 0.34% p16+ nuclei, and in the recurrent varicose vein group, we found 0.74% p16+ nuclei. At the p < 0.05 level, the three groups tended to be significant without reaching statistical significance ( p = 0.085). There was no difference in respect of p21 and Ki67. Conclusion We found significantly higher expression rates of p53 in primary varicose veins at the saphenofemoral junction than in healthy veins. p16 expression tended to be increased in the recurrent varicose vein group. These preliminary findings indicate that cellular senescence may have an impact in the development of varicose veins or recurrence. Further studies addressing this issue are necessary.

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Plasma concentration of selected biochemical markers of endothelial dysfunction in women with various severity of chronic venous insufficiency (CVI)—A pilot study

Cited by 15 publications

References 68 publications

Identification of Endothelial Proteins in Plasma Associated With Cardiovascular Risk Factors

Identification of Endothelial Proteins in Plasma Associated With Cardiovascular Risk Factors

Higher serum Endocan levels are involved in the pathophysiology of chronic venous insufficiency

Cellular senescence at the saphenofemoral junction in patients with healthy, primary varicose and recurrent varicose veins – A pilot study

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