Plasma concentration of intestinal- and liver-FABP in neonates suffering from necrotizing enterocolitis and in healthy preterm neonates

Guthmann, Florian; Börchers, Torsten; Wolfrum, Christian; Wustrack, Thomas; Bartholomäus, Sabine; Spener, Friedrich

doi:10.1007/978-1-4419-9270-3_29

Cited by 54 publications

(79 citation statements)

References 16 publications

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“…Plasma concentrations of the intestinal fatty-acid binding protein 2 are elevated in patients with NEC (31), and this may relate to our observation that intestinal fatty-acid binding protein 5 expression was reduced in NEC intestines. The role of this protein remains unclear apart from its known role in fattyacid absorption and transport and in local nervous system and regulation of water permeability (32).…”

Section: Articlesmentioning

confidence: 66%

Intestinal proteome changes during infant necrotizing enterocolitis

et al. 2012

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Background: changes in the intestinal and colonic proteome in patients with necrotizing enterocolitis (Nec) may help to characterize the disease pathology and identify new biomarkers and treatment targets for Nec. Methods: Using gel-based proteomics, proteins in Necaffected intestinal and colonic sections were compared with those in adjacent, near-normal tissue sections within the same patients. Western blot and immunohistochemistry were applied to crossvalidate proteomic data and histological location of some selected proteins. results: Thirty proteins were identified with differential expression between necrotic and vital small-intestine sections and 23 proteins were identified for colon sections. Five proteins were similarly affected in the small intestine and colon: histamine receptors (hRs), actins, globins, immunoglobulin, and antitrypsin. Two heat shock proteins (hsPs) were affected in the small intestine. Furthermore, proteins involved in antioxidation, angiogenesis, cytoskeleton formation, and metabolism were affected. Finally, secretory proteins such as antitrypsin, fatty-acid binding protein 5, and haptoglobin differed between Nec-affected and vital tissues. conclusion: Nec progression affects different pathways in the small intestine and colon. hsPs may play an important role, especially in the small intestine. The identified secretory proteins should be investigated as possible circulating markers of Nec progression in different gut regions.

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Section: Articlesmentioning

confidence: 66%

Intestinal proteome changes during infant necrotizing enterocolitis

et al. 2012

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“…Since most of these tests are abnormal in these patients, none of these tests are specifi c and sensitive enough to make a diagnosis in early stages. Recently, some cytosolic enzymes like glutathione S-transferase and some biochemical parametres located in intestinal mucosal villi like intestinal fatty acid binding protein (I-FABP) are being questioned for usage in AMI as a serological indicator (27,28). Although these studies have promising results, their usage in clinic practice does not seem to be possible and practical.…”

Section: Discussionmentioning

confidence: 99%

The place of D-dimer and L-lactate levels in the early diagnosis of acute mesenteric ischemia

Aydın¹,

Ozban²,

Serinken³

et al. 2015

BLL

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INTRODUCTION: Acute mesenteric ischemia (AMI) is an abdominal-vascular emergency which is rare and has high mortality rates (60-80 %) due to late diagnosis (1-3). Although it is known that extravascular reasons like intestinal intussusception, volvulus, strangulated hernias and obstructions can cause intestinal gangrene, these are rarely the cause of AMI (1). MATERIALS AND METHODS: In this study, we used male Wistar-Albino rats weighing 250-300 grams obtained from Pamukkale University Experimental Research Laboratory. Animals were exposed to light-dark cycles for 12 hours and had free access to food and water. They were kept in cages for 7 days to stabilise their intestinal fl ora. In animals of group I, nothing was made other than taking 0.5 ml blood intracardially. In other animals, abdomen was reached with midline laparotomy and superior mesenteric artery (SMA) was located. In group II (operative control group), SMA was isolated and manipulated but was not ligated. In Group III (intestinal ischemia group), SMAwas isolated and ligated with 3/0 silk tie distally to the aorta. After this process, intestinal ischemia was achieved which was confi rmed by paleness and pulselessness of intestines, caecum and right colon. Later on, abdomen was closed with double 3/0 polyglactin sutures. At postoperative 1st, 4th and 6th hours 0.5 ml blood was taken intracardially from the animals in groups II and III in order to quantify D-dimer and L-lactate levels. LABORATORY TESTS: D-dimer: Blood samples which were put into tubes containing sodium citrate, were seperated from plasma with centrifugation at 4000 rpm for 7 minutes. L-lactate: Blood L-lactate levels were determined from blood taken into capillary tubes with the help of immobilised enzyme electrode technology using YSI 1500 Sport portative lactate analyzer (Yellow Springs Instruments Inc., Ohio-USA). HISTOPATHOLOGIC VERIFICATION: Two cm long intestinal samples were taken from animals in which SMA was ligated in order to achieve mesenteric ischemia and these samples were fi xed in 10 % formol. DISCUSSION: As a result, in rats with SMA occlusion serum D-dimer levels were not increased signifi cantly when compared either in the group or with the basal values of the control group and values in operative control group. Therefore, it is concluded that D-dimer is not a useful marker for early diagnosis of AMI. On the other hand, it is revealed that blood L-lactate levels began to increase signifi cantly following 4th hour of mesenteric ischemia and it is shown that this increase continued at the 6th hour. In addition, considering the utmost importance of the early diagnosis in patients with the clinical suspicion of AMI, L-lactate seems to be a suitable marker to use in emergency departments because it is achieved with a portable device that gives fast and accurate results. Nevertheless, our results are need to be supported by clinical studies with larger patient series (Tab. 2, Fig. 11, Ref. 39). Text in PDF www.elis.sk.

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“…NT-proBNP is supposed to represent a cardiac marker of hypoperfusion, H-FABP a potential marker of cardiac and intestinal hypoperfusion [15,16] . Increased levels of NGAL were hypothesized to indicate renal hypoperfusion as a result of a steal phenomenon caused by re-circulation of blood across an open duct, thus negatively affecting blood flow to the kidneys [12,17] .…”

Section: Introductionmentioning

confidence: 99%

Urinary NT-proBNP, NGAL, and H-FABP May Predict Hemodynamic Relevance of Patent Ductus Arteriosus in Very Low Birth Weight Infants

Tosse¹,

Pillekamp²,

Verde

et al. 2012

Neonatology

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Background: Hemodynamically significant patent ductus arteriosus (hsPDA) is the most common functional cardiovascular disease in preterm infants. The necessity to treat hsPDA can neither be derived solely from clinical nor from echocardiographic criteria. Objective: The aim of this study was to establish non-invasive parameters which can differentiate hsPDA from non-hsPDA. Methods: Urinary protein levels of NT-proBNP, NGAL, and H-FABP were measured and correlated with the necessity of therapy for PDA. In 37 neonates (<1,500 g), urinary protein concentrations were tested on days 0, 2, and 7 by ELISA methodology. Of 37 infants, 12 required therapeutic interventions according to current treatment standards. Results: Infants receiving an intervention for PDA showed significantly higher levels of pro-BNP, NGAL, and H-FABP at all time points except for NT-proBNP on day 0. Infants requiring a second or third course of ibuprofen had significantly higher levels of H-FABP and NGAL. In all samples, the concentration of the three proteins correlated positively with each other. Conclusions: The present study shows that measurement of urinary proteins is a powerful and non-invasive method to quantify the effect of PDA on systemic perfusion in preterm infants. Furthermore, NGAL and H-FABP may be used to indicate the necessity of pharmacological or surgical treatment of PDA.

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Plasma concentration of intestinal- and liver-FABP in neonates suffering from necrotizing enterocolitis and in healthy preterm neonates

Cited by 54 publications

References 16 publications

Intestinal proteome changes during infant necrotizing enterocolitis

Intestinal proteome changes during infant necrotizing enterocolitis

The place of D-dimer and L-lactate levels in the early diagnosis of acute mesenteric ischemia

Urinary NT-proBNP, NGAL, and H-FABP May Predict Hemodynamic Relevance of Patent Ductus Arteriosus in Very Low Birth Weight Infants

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