Plasma Biochemistry values in wild female hawksbill turtle (Eretmochelys imbricata) during nesting in Mexican coast

Abstract

To obtain plasma biochemistry values, blood was collected for 47 nesting females of apparently healthy Eretmochelys imbricata sea turtles using sodium heparin as an anticoagulant. Blood samples were collected in April-Jun for two years (nesting season). Hematologic characteristics, including packed cell volume, white blood cell counts, red blood cell count and hemoglobin level; and plasma chemistry values, including creatinine, blood urea nitrogen, uric acid, triglyceride, total cholesterol, and glucose were measured. The data generated from this study may be useful for clinical assessment of health and disease of wild hawksbill sea turtles on nearshore habitats in the Gulf of Mexico, thus contributing to a conservation of this species. Keywords: Eretmochelys imbricata. Hematology. Plasma chemistry. Conservation. ResumoPara obter valores da bioquímica plasmática, foram coletadas amostras de sangue durante a desova de 47 tartarugas marinhas fêmeas aparentemente saudáveis da espécie Eretmochelys imbricata, utilizando heparina sódica como anticoagulante. Amostras de sangue foram coletadas durante dois anos entre os meses de abril e junho (época de nidificação). Mediu-se os parâmetros hematológicos (incluindo hematócrito, contagem de glóbulos brancos e vermelhos e nível de hemoglobina) e os valores da bioquímica plasmática (incluindo creatinina, ureia, ácido úrico, triglicérides, colesterol total e glicose). Os dados gerados a partir deste estudo podem ser úteis para a avaliação clínica de saúde e de doença em tartarugas-de-pente em habitats próximos ao litoral no Golfo do México, contribuindo para a conservação dessa espécie. Palavras-chave: Eretmochelys imbricata. Hematologia. Bioquímica Plasmática. Conservação.

Hawksbill Sea Turtle (Eretmochelys imbricata) Blood and Eggs Organochlorine Pesticides Concentrations and Embryonic Development in a Nesting Area (Yucatan Peninsula, Mexico)

“…Plasma biochemistry data are provided in Figure 3 , with values reported for blood collected in lithium heparin. Most of the biochemical parameters have a significant correlation with biometric factors ( p < 0.05) with CCL: urea ( p -value = 0.494); creatinine ( p -value = 0.4227), glucose ( p -value = 0.4554), cholesterol ( p -value = 0.08054), uric acid ( p -value = 0.9309) and triglyceride ( p -value = 0.4908) [ 90 ].…”

“…For the hematological and biochemical analysis, the biological material that had been collected was sent to and processed in the Central Laboratory of Animal Pathology of Campeche (LACEPAC) (Campeche, México). Plasma biochemistry determinations included cholesterol, glucose, triglycerides, urea, creatinine, and uric acid [ 90 ]. All contaminant analysis was performed at the Institute of Ecology, Fishery, and Oceanography of the Gulf of Mexico (EPOMEX, Campeche, Mexico).…”

“…The objective of this study was to determine OCP concentrations in the blood and eggs of the hawksbill turtle Eretmochelys imbricata , and the relationship between the concentrations of contaminants and the nesting turtle population’s reproductive performance-specifically, maternal transference and embryonic development (total eggs, number of offspring, offspring/eggs ratio, the weight of the whole egg, weight content, and shell) in relation to sea turtle size (CCL = curved carapace length; CCW = curved carapace width). In addition, we evaluated the turtles’ health state during the breeding season and produced working reference intervals for the hematologic and plasma biochemical parameters of nesting hawksbill sea turtles along the Mexican coast (Punta Xen) [ 90 ]. Physical examinations, hematology, and the plasma biochemistry reference ranges of biochemical parameters were taken to assess and monitor the health status of sea turtles and to create suitable environmental indicators to improve the effectiveness of conservation strategies.…”

Hematological and Biochemical Reference Intervals for Euphrates Softshell Turtle (Rafetus euphraticus)

“…Serum urea nitrogen concentration was measured using a coupled urease/glutamate dehydrogenase kinetic system. Cholesterol was estimated based on the enzyme-driven reaction that quantifies both cholesterol esters and free cholesterol (Rifai, Bachorik, & Albers, 1999). Triglyceride levels were measured based on the enzymatic hydrolysis of triglycerides by lipase to produce glycerol and free fatty acids (Rifai, Bachorik, & Albers, 1999).…”

“…Cholesterol was estimated based on the enzyme-driven reaction that quantifies both cholesterol esters and free cholesterol (Rifai, Bachorik, & Albers, 1999). Triglyceride levels were measured based on the enzymatic hydrolysis of triglycerides by lipase to produce glycerol and free fatty acids (Rifai, Bachorik, & Albers, 1999). Phosphorus ions were estimated by reacting with ammonium molybdate in the presence of sulfuric acid and monitored at 340 nm (Endres and Rude 1999).…”