Plasma-assisted synthesis of noble nanoparticles coated with hyaluronan: chemico-physical characterization and safety assessment

Abstract

Nanosystems are gaining attention rapidly in the biomedical, chemical, material, computer, and catalysis field. Recent researches have been focused on synthetic methods to decrease toxicity and side effects respect to classic formulations. Although, the harmonization in interpretative criteria of biological activity is limited as well as the strategies to improve the scalability of synthetic technologies. The present work described the use of atmospheric plasma as a promising strategy to produce size-controlled and safe noble nanoparticles, which could not show surface toxicity due to absorption of chemicals during the synthesis reaction. The physisorption with Hyaluronic Acid was used to modulate nanoparticles aggregation kinetic and improve biological properties. Physico-chemical characterization has been conducted using NMR spectroscopy, UV-visible and dynamic light scattering. Cytotoxicity on bacterial and HUVEC cells has been tested. The results demonstrated the efficiency of plasma synthetic method to control nanoparticles size and toxicity, improving selectively antibacterial activity against Gram-negative.