Plasma as a Scaffold for Regeneration of Neural Precursor Cells after Transplantation into Rats with Spinal Cord Injury

Abstract

The present study investigated whether plasma could be useful as a scaffold for cell transplantation in rats with spinal cord injury (SCI). Transplantation of cells with plasma promoted the recovery of SCI-induced motor dysfunction. Immunohistochemical analysis revealed that the grafted cells had differentiated into the neural lineage. When dissociated neural precursor cells were cultured with plasma, extensive neurite outgrowth was observed along with increased expression of p35 and NF68. Neural markers were also expressed by the cultured cells. Culture with plasma reduced thymidine incorporation, but promoted cell growth and increased the RNA contents. These findings suggest that the cells underwent differentiation into neurons in the presence of plasma. In conclusion, plasma could be a promising scaffold for cell transplantation therapy.

Matrigel supports survival and neuronal differentiation of grafted embryonic stem cell‐derived neural precursor cells

“…However, because MG is derived from mouse sarcomas, it cannot be used clinically owing to potential immunogenicity and pathogen transmission. For clinical applications, a human-derived matrix, such as the amniotic membrane that supports neural differentiation from ES cells (Ueno et al, 2006), plasma (Takenaga et al, 2007), or synthetic biomaterials must be developed.…”

The Effect of Am-80, a Synthetic Retinoid, on Spinal Cord Injury-Induced Motor Dysfunction in Rats

“…The room had a 12 h light/dark cycle. The study protocol was approved by the An- Spinal Cord Injury and Drug Treatment Impact injury of the spinal cord was induced by using the weight-drop device developed at New York University (NYU impact model), according to the method described by Takenaga et al 26,27) and Kitagawa et al 28) Adult female SD rats weighing 210-230 g were anesthetized and dorsal laminectomy was performed at the T 10 level, after which a 10 g weight was dropped onto the spinal cord from a height of 25 mm.…”

Self-assembling peptides optimize the post-traumatic milieu and synergistically enhance the effects of neural stem cell therapy after cervical spinal cord injury

“…Scaffolds, such as collagens, fibrin or plasma, represent an exciting bioengineered strategy to bridge the lesion cavity and shape the inhibitory post-traumatic microenvironment [19]. Agarose hydrogels or alginates reduce astroglial and fibrotic scarring and serve as a matrix to deliver drugs or growth factors, both supporting outgrowth and regeneration of axons [20][21][22][23].…”