Plasma and serum serotonin concentrations and surface-bound platelet serotonin expression in Cavalier King Charles Spaniels with myxomatous mitral valve disease

Abstract

A substantive influence of circulating serotonin on MMVD stages prior to CHF development in Cavalier King Charles Spaniels was not supported by the study findings. An FSSP of highly activated platelets with pronounced serotonin binding was strongly associated with thrombocytopenia but not MMVD.

Analytical validation of platelet microparticle quantification in cats

“…PMPs were defined as CD61‐positive events within P2, using an APC‐conjugated anti‐CD61 antibody (Clone Y2/51; DAKO Cytomation, Glostrup, Denmark) for CD61 detection. Preliminary data demonstrated satisfactory platelet binding, very similar to that seen in dogs . (Figure S2).…”

“…Preliminary data demonstrated satisfactory platelet binding, very similar to that seen in dogs. 36 (Figure S2). A corresponding isotype control antibody (mouse IgG1-APC ×0968; DAKO Cytomation) was used to define the nonspecific binding and the negative cutoff for CD61-positive events (events in P3; Figure S1C Voltage drift for APC, FITC, PE, PE-Cy, side scatter, and forward scatter was checked daily.…”

Alpha-smooth muscle actin and serotonin receptors 2A and 2B in dogs with myxomatous mitral valve disease

“…This can likely be explained by the lack of young dogs with none or earlystage MMVD in the present study, as these disease-stages have been associated with increased serum and plasma 5-HT concentrations (Cremer et al, 2015b).…”

A pilot study investigating circulating trimethylamineN‐oxide and its precursors in dogs with degenerative mitral valve disease with or without congestive heart failure

“…The disease process has been well studied, but factors contributing to both underlying cause and disease progression remain elusive . A genetic predisposition exists for the disease (eg, Cavalier King Charles Spaniels, and Dachshunds), but several other factors have been postulated to play a role, including nutrition, inflammation, and neurohumoral factors, such as serotonin …”