Plasma and Liver Hepatitis C Virus Variability in Patients Coinfected with Human Immunodeficiency Virus

Jouvencel, Anne-Christine; Néau, Didier; Faure, Muriel; Neau, Martine; Martinaud, Christophe; Legrand, E.; Trimoulet, Pascale; Garrigue, Isabelle; Bail, Brigitte Le; Bioulac‐Sage, Paulette; Dupon, M.; Ragnaud, Jean‐Marie; Fleury, Hervé; Lafon, Marie‐Edith

doi:10.1128/jcm.44.5.1877-1880.2006

Cited by 6 publications

(6 citation statements)

References 25 publications

(21 reference statements)

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“…Díaz Carrasco et al 5 of sequences present in plasma would be able to associate with these cells, a phenomenon called viral compartmentalization. Compartmentalized HCV sequences have been demonstrated both in immunocompromised (18,(22)(23) and immunocompetent (24)(25) individuals, and in the setting of liver transplantation (18,(26)(27). Additionally, PBMC-associated HCV sequences have also been detected after resolution of HCV infection thus indicating occult infection (28-29).…”

Section: Introductionmentioning

confidence: 99%

Dynamic changes in viral population structure and compartmentalization during chronic hepatitis C virus infection in children

et al. 2013

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Classic phylogenetic and modern population-based clustering methods were used to analyze hepatitis C virus (HCV) evolution in plasma and to assess viral compartmentalization within peripheral blood mononuclear cells (PBMCs) in 6 children during 3.2-9.6yr of follow-up. Population structure analysis of cloned amplicons encompassing hypervariable region 1 led to the distinction of two evolutionary patterns, one highly divergent and another one genetically homogeneous. Viral adaptability was reflected by co-evolution of viral communities switching rapidly from one to another in the context of divergence and stability associated with highly homogeneous communities which were replaced by new ones after long periods. Additionally, viral compartmentalization of HCV in PBMCs was statistically demonstrated, suggesting their role as a pool of genetic variability. Our results support the idea of a community-based structure of HCV viral populations during chronic infection and highlight a role of the PBMC compartment in the persistence of such structure.

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Section: Introductionmentioning

confidence: 99%

Dynamic changes in viral population structure and compartmentalization during chronic hepatitis C virus infection in children

et al. 2013

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“…20,21 Some differences between serum and liver quasispecies have been sought but inconsistently described. [22][23][24] The distribution of liver quasispecies may also differ when HCC and nontumoral cirrhotic liver are compared. [25][26][27][28][29][30] Finally, some articles have shown that peripheral blood mononuclear cells or hepatic lymph nodes can harbor minor or major variants not found in the serum.…”

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confidence: 99%

Distinct hepatitis C virus core and F protein quasispecies in tumoral and nontumoral hepatocytes isolated via microdissection

et al. 2007

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Hepatitis C virus (HCV) genetic variability may be involved in liver carcinogenesis. We investigated HCV core and corresponding putative F protein genetic variability in hepatocellular carcinoma (HCC) and cirrhotic nodules. Hepatocyte clusters from 7 patients with HCC and HCV1b-related cirrhosis were isolated via microdissection of HCC tissues and 2 nontumoral cirrhotic nodules. The HCV core complementary DNA was cloned and sequenced from each liver compartment and from the serum of 2 patients. Nucleotide diversity and synonymous and nonsynonymous substitutions were analyzed within and between compartments via phylogenetic analysis and Mantel's test. Liver HCV RNA accumulation was lower in HCC. Increased quasispecies diversity and complexity was observed with HCC in 6 of 7 patients. Mantel's test demonstrated marked compartmentalization of quasispecies between HCC and cirrhotic nodules in all 7 patients and also between the 2 nontumoral nodules in 5 of them. Synonymousnonsynonymous substitution analysis indicated low selection against tumoral core quasispecies in all patients and a more selective pressure against F protein quasispecies in all compartments. In the 2 subjects analyzed, HCC and nontumoral hepatocyte quasispecies were only minor or undetected in serum. Conclusion: In tumoral hepatocytes, low-replicating hepatitis C quasispecies are compartmentalized and more diversified and are subjected to low selective pressure. Our study supports the importance of core genetic variability in hepatocellular carcinogenesis.

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“…The characteristics of the four patients analyzed are expressed in Table 1. A total of 414 cloned sequences were obtained and an average of 17 (11)(12)(13)(14)(15)(16)(17)(18)(19)(20)(21)(22)(23)(24) sequences from each sample for both of the genomic regions analyzed (Table 1).…”

Section: Resultsmentioning

confidence: 99%

“…samples. Although circulating virus is thought to represent liver population, some studies have shown evidences that plasma variants differed from those in the liver [18,19]. Moreover, a few studies have found different viral variants according to tumoral or nontumoral tissue sub-localization within the liver of HCC patients [20][21][22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Compartmentalization of hepatitis C virus variants in patients with hepatocellular carcinoma

Pérez

Lello

Mullen

et al. 2016

Molecular Carcinogenesis

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Chronic Hepatitis C Virus (HCV) infection is a major risk for hepatocellular carcinoma (HCC) development. HCV Core protein has been associated with the modulation of potentially oncogenic cellular processes and E2 protein has been useful in evolutive studies to analyze the diversity of HCV. Thus, the aim of this study was to evaluate HCV compartmentalization in tumoral, non-tumoral liver tissue and serum and to identify viral mutations potentially involved in carcinogenesis. Samples were obtained from four patients with HCC who underwent liver transplantation. Core and E2 were amplified, cloned and sequenced. Phylogenies and BaTS Test were performed to analyze viral compartmentalization and a signature sequence analysis was conducted by VESPA. The likelihood and Bayesian phylogenies showed a wide degree of compartmentalization in the different patients, ranging from total clustering to a more scattered pattern with small groups. Nevertheless, the association test showed compartmentalization for the three compartments and both viral regions tested in all the patients. Signature amino acid pattern supported the compartmentalization in three of the cases for E2 protein and in two of them for Core. Changes observed in Core included polymorphism R70Q/H previously associated with HCC. In conclusion, evidence of HCV compartmentalization in the liver of HCC patients was provided and further biological characterization of these variants may contribute to the understanding of carcinogenesis mediated by HCV infection. © 2016 Wiley Periodicals, Inc.

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Plasma and Liver Hepatitis C Virus Variability in Patients Coinfected with Human Immunodeficiency Virus

Cited by 6 publications

References 25 publications

Dynamic changes in viral population structure and compartmentalization during chronic hepatitis C virus infection in children

Dynamic changes in viral population structure and compartmentalization during chronic hepatitis C virus infection in children

Distinct hepatitis C virus core and F protein quasispecies in tumoral and nontumoral hepatocytes isolated via microdissection

Compartmentalization of hepatitis C virus variants in patients with hepatocellular carcinoma

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