Plasma alpha 1‐acid glycoprotein concentration in broilers: Influence of age, sex and injection ofescherichia colilipopolysaccharide

Abstract

1. The influence of age, sex and injection of Escherichia coli lipopolysaccharide (LPS) on the plasma concentration of alpha-1 acid glycoprotein (AGP) was determined in broilers using the single radial immunodiffusion method. 2. Plasma AGP concentration increased in the 3 d after hatching, and then stabilised at 240 +/- 33 micrograms/ml up to 14 d of age. 3. No sex-related differences in plasma AGP concentration were observed up to 6 weeks of age. 4. A single injection of 900 micrograms LPS per chick resulted in a 5-fold increase in AGP concentration compared with that in saline-injected chicks. Multiple injections of LPS (200 micrograms/chick every 2 d for 14 d) caused only a 50% increase in AGP concentration.

Role of Chicken TL1A on Inflammatory Responses and Partial Characterization of Its Receptor

“…Cer and ␣ 1 AG concentrations were increased (Fig. 5, A and B); these changes being similar to those seen after LPS injection (13)(14)(15). ChTL1A fusion protein and GST preparations did not contain detectable levels of LPS (Ͻ100 ng/mg protein) using an Endospecy ES-50M kit (Seikagaku).…”

“…Thus, proinflammatory cytokines are also important regulators of metabolic responses in the acute phase of inflammation. In chickens, ␣ 1 AG, Cer, and NO are acute phase substances released following LPS injection (13,14,25). Our results demonstrate that ChTL1A promotes inflammation by raising these.…”

Swollen head syndrome in broiler chickens in Japan: Its pathology, microbiology and biochemistry

“…In chickens, Takahashi et al (1994) reported first that the injection of E. coli lipopolysaccharide induced an increase in plasma a'-AG level. Nakamura et al (1995) reported that IB virus and infectious laryngotracheitis virus caused an elevation of serum a'-AG levels.…”

“…'Chicken a'-AG plates' (Saikin Kagaku Institute Co Ltd, Sendai, Japan), incorporating the specific anti-chicken a'-AG serum of rabbit origin in agarose gel, were used for measurement of chicken a'-AG (Nakamura et al, 1995;Takahashi et al, 1994). This is a single radial immunodiffusion method.…”

Investigation of serum amino acid and serum amyloid A concentrations in chickens with amyloid arthropathy

“…Altered health status of birds often results in measurable changes in blood biochemistry (Hurwitz and Griminger 1961;Okumura and Tasaki 1969), measuring plasma levels of certain acute-phase proteins (Kushner and Mackiewiez 1987;Singh 1988;Saini and Webert 1991;Takahashi et al 1994;Tohyo et al 1995Tohyo et al , 1996, including the SAA protein also (Chamanza et al 1999;Gruys et al 2005), could be useful in monitoring poultry health. It has been suggested that increased circulating proteolytic cleavage of SAA may potentially contribute to the development of AA amyloid deposition (Migita et al 1996).…”