Plasma activated coatings with dual action against fungi and bacteria

Akhavan, Behnam; Michl, Thomas D.; Giles, Carla; Ho, Kaitlyn; Martin, L.; Sharifahmadian, Omid; Wise, Steven G.; Coad, Bryan R.; Kumar, Naresh; Griesser, Hans J.; Bilek, M.M.M.

doi:10.1016/j.apmt.2018.04.003

Cited by 53 publications

(46 citation statements)

References 69 publications

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“…The instrument used was a Physical Electronics Inc. PHI TRIFT V nanoToF with a pulsed liquid 79 + Au primary ion gun that was operated at 30 kV . Dual charge neutralization was achieved in the form of an electron flood gun and Ar + ions (both operating at 10 eV).…”

Section: Methodsmentioning

confidence: 99%

An Acid Test: Facile SI‐ARGET‐ATRP of Methacrylic Acid

Michl

Jung

Pertoldi

et al. 2018

Macro Chemistry & Physics

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Atom transfer radical polymerization (ATRP) of methacrylic acid (MAA) is challenging. Herein is reported a study of conditions for facile surface‐initiated ATRP by activator regenerated electron transfer (SI‐ARGET‐ATRP) growth of poly‐methacrylic acid (PMAA) chains from a plasma polymer surface bearing surface‐immobilized α‐bromoisobutyryl bromide, with no deoxygenation required. Factors that affect PMAA polymer growth off the surface under ARGET‐ATRP conditions are systematically investigated, such as monomer/catalyst ratio, solvent, and, most importantly, addition of salts and change of pH. While the concentrations of the copper catalyst and acid affect grafting, the most pronounced effect arises from the concentration of chloride ions. Adding 0.1 m NaCl and acidifying the reaction solution to pH 3 offers the best trade‐off between reaction rate and reproducibility; yielding ≈60 nm thick PMAA graft polymers in 1 h under ambient conditions. Using this easily scalable recipe and surface analysis, the grafted polymers are verified to be pure PMAA and the graft coatings to be homogenous across a substrate of 100 mm diameter.

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Section: Methodsmentioning

confidence: 99%

An Acid Test: Facile SI‐ARGET‐ATRP of Methacrylic Acid

Michl

Jung

Pertoldi

et al. 2018

Macro Chemistry & Physics

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“…[ 43,44 ] For nonpolymeric substrates, such as metals and ceramics, PIII can be combined with plasma polymerization to generate reactive, radical‐rich coatings. [ 47–51 ] Over the last decade, we have used PIII for reagent‐free, covalent attachment of biomolecules, such as proteins, peptides, and DNA on surfaces. [ 12,52–56 ] The covalent attachment occurs when molecules in physical contact with the surface react with surface‐embedded radicals generated by the implanted ions.…”

Section: Introductionmentioning

confidence: 99%

Hydrogel−Solid Hybrid Materials for Biomedical Applications Enabled by Surface‐Embedded Radicals

Walia

Akhavan

Kosobrodova

et al. 2020

Adv Funct Materials

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Combinations of hydrogels and solids provide high level functionality for devices such as tissue engineering scaffolds and soft machines. However, the weak bonding between hydrogels and solids hampers functionality. Here, a versatile strategy to develop mechanically robust solid−hydrogel hybrid materials using surface embedded radicals generated through plasma immersion ion implantation (PIII) of polymeric surfaces is reported. Evidence is provided that the reactive radicals play a dual role: inducing surface‐initiated, spontaneous polymerization of hydrogels; and binding the hydrogels to the surfaces. Acrylamide and silk hydrogels are formed and covalently attached through spontaneous reactions with the radicals on PIII activated polymer surfaces without cross‐linking agents or initiators. The hydrogel amount increases with incubation time, monomer concentration, and temperature. Stability tests indicate that 95% of the hydrogel is retained even after 4 months in PBS solution. T‐peel tests show that failure occurs at the tape−hydrogel interface and the hydrogel‐PIII‐treated PTFE interfacial adhesion strength is over 300 N m−1. Cell assays show no adhesion to the as‐synthesized hydrogels; however, hydrogels synthesized with fibronectin enable cell adhesion and spreading. These results show that polymers functionalized with surface‐embedded radicals provide excellent solid platforms for the generation of robust solid−hydrogel hybrid structures for biomedical applications.

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“…CAP is an ionized gas near room temperature, which consists of various molecules, radicals, ions, electrons, excited species, electric field, and UV radiation. Previous studies have reported that CAP exhibited antimicrobial activity in bacteria [ 7 – 10 ] and induced angiogenesis by regulating wound healing molecular expression in skin cells [ 11 – 13 ]. In particular, CAP contains highly reactive oxygen and nitrogen species (RONS), and it can induce cell death by causing oxidative damage to cancer cells.…”

Section: Introductionmentioning

confidence: 99%

Anticancer Effects of Plasma-Activated Medium Produced by a Microwave-Excited Atmospheric Pressure Argon Plasma Jet

Joh

Chung

et al. 2020

Oxidative Medicine and Cellular Longevity

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Cold atmospheric plasma (CAP) has been reported to have strong anticancer effects in vitro and in vivo. CAP has been known to induce apoptosis in most cancer cells by treatment to cells using direct and indirect treatment methods. There are many reports of apoptosis pathways induced by CAP, but for indirect treatment, there is still a lack of fundamental research on how CAP can cause apoptosis in cancer cells. In this study, we applied an indirect treatment method to determine how CAP can induce cancer cell death. First, plasma-activated medium (PAM) was produced by a 2.45 GHz microwave-excited atmospheric pressure plasma jet (ME-APPJ). Next, the amounts of various reactive species in the PAM were estimated using colorimetric methods. The concentration of NO2– and H2O2 in PAM cultured with cancer cells was measured, and intracellular reactive oxidative stress (ROS) changes were observed using flow cytometry. When PAM was incubated with A549 lung cancer cells, there was little change in NO2– concentration, but the concentration of H2O2 gradually decreased after 30 min. While the intracellular ROS of A549 cells was rapidly increased at 2 hours, there was no significant change in that of PAM-treated normal cells. Furthermore, PAM had a significant cytotoxic effect on A549 cells but had little effect on normal cell viability. In addition, using flow cytometry, we confirmed that apoptosis of A549 cells occurred following flow cytometry and western blot analysis. These results suggest that among various reactive species produced by PAM, hydrogen peroxide plays a key role in inducing cancer cell apoptosis.

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Plasma activated coatings with dual action against fungi and bacteria

Cited by 53 publications

References 69 publications

An Acid Test: Facile SI‐ARGET‐ATRP of Methacrylic Acid

An Acid Test: Facile SI‐ARGET‐ATRP of Methacrylic Acid

Hydrogel−Solid Hybrid Materials for Biomedical Applications Enabled by Surface‐Embedded Radicals

Anticancer Effects of Plasma-Activated Medium Produced by a Microwave-Excited Atmospheric Pressure Argon Plasma Jet

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