Plasma 8-Isoprostane Concentrations and Adipogenic and Adipokine Gene Expression Patterns in Subcutaneous and Mesenteric Adipose Tissues of Fattening <i>Wagyu</i> Cattle

Abstract

ABSTRACT. We hypothesized that fattening Wagyu cattle fed conventional low-vitamin fattening diets are exposed to oxidative stress. In this experiment, we studied the plasma concentrations of 8-isoprostane and the fat depot-specific effects of the diet-induced adipogenic (C/ EBPβ, C/EBPδ, C/EBPα and PPARγ2) and adipokine (VEGF, FGF-2, leptin and adiponectin) gene expressions in fattening Wagyu steers. Animals were fed a high-vitamin (α-tocopherol and β-carotene) diet (HV) or a control diet (CT) during the fattening period (from 10 to 30 months of age). The plasma concentrations of 8-isoprostane, a marker of oxidative stress, were significantly lower in the HV group than in the CT group. In mesenteric adipose tissue, the expressions of the adipogenic and adipokine genes in the HV group were significantly lower than those in the CT group. In contrast, there were no differences in the expression of the adipogenic and adipokine genes in subcutaneous adipose tissue between groups. These results suggest that higher intake of dietary α-tocopherol and β-carotene affects the expression patterns of adipogenic and adipokine genes in a fat depot-specific manner with the reduction of plasma 8-isoprostane concentrations.

Regulatory expression of components in the BMP pathway in white adipose tissues of cattle

“…The dietary treatments employed were the same as those reported by Yamada and Nakanishi (2012) and Asano et al (2013) for experiment (Exp) 1 and Yamada et al (2013) and Kanamori et al (2014) for Exp 2.…”

Effects of Whole Crop Rice Silage on Meat Quality and Adipokine Gene Expression in Fattening <i>Wagyu</i> Cattle

“…The growth performance and carcass characteristics of Wagyu steers in this experiment were described in our previous reports (Yamada et al 2012, Yamada et al 2013). In brief, there were no significant differences in the final body weight and daily weight gain between the groups.…”

“…The experimental design was described in detail in our previous reports (Yamada et al 2012, Yamada et al 2013. In brief, at 10 months of age, Wagyu steers were divided into two groups.…”

“…(2) The high-vitamin diet group (HV, n=4) was fed total mixed rations (TMR) containing WCRS (Hamasari at the yellow ripe stage), which consisted of 72% TDN and 60 mg/kg of α-tocopherol (fed from 10 to 20 months), and 77% TDN and 22 mg/kg α-tocopherol (fed from 21 to 30 months). At 30 months of age, blood samples were collected, and the plasma levels of 8-isoprostane were measured using an ELISA kit (Yamada et al 2013). At 30 months of age, the steers were slaughtered, and the longissimus muscle, subcutaneous adipose tissue, and mesenteric adipose tissue were sampled.…”

“…The α-tocopherol concentrations, metmyoglobin (Mb) percentages, and values of 2-thiobarbituric acid-reactive substances (TBARS) in the longissimus muscle were measured as previously described (Yamada et al 2012). Adipokine gene expressions in subcutaneous and mesenteric adipose tissue were analyzed by real-time PCR (Yamada et al 2013). …”