Plasma 11-oxotestosterone and gonadotropin during the beginning of spermiation in rainbow trout (Salmo gairdneri R.)

Fostier, Alexis; Billard, Roland; Breton, Bernard; Legendre, Marc; Marlot, Sylviane

doi:10.1016/0016-6480(82)90096-x

Cited by 99 publications

(49 citation statements)

References 24 publications

(22 reference statements)

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“…Levels of 11-ketotestosterone (11KT) were measured by specific RIA in culture media according to Fostier et al (1982). Each sample was assayed in duplicates.…”

Section: Steroid Measurementmentioning

confidence: 99%

Fsh and Lh have common and distinct effects on gene expression in rainbow trout testis

Sambroni¹,

Rolland²,

Lareyre³

et al. 2012

Journal of Molecular Endocrinology

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The general rules established from mammalian species for the regulation of spermatogenesis by gonadotropins may not be fully relevant in fish. Particularly, Fsh is as potent as Lh to stimulate steroidogenesis and the Fsh receptor is expressed in Leydig cells. In seasonal breeders, Fsh is likely the major gonadotropin involved in spermatogenesis onset and Lh is required to support spermatogenesis progression and gamete release. However, the genes that relay the action of Fsh and Lh have been poorly investigated in fish. The present study was aimed at identifying gonadotropin-dependent genes expressed in the testis during fish puberty. We cultured pubertal trout testicular explants for 96 h, with or without gonadotropin, and analyzed transcriptome variations using microarrays. Fsh and Lh had similar effects on a large group of genes while other genes were preferentially regulated by one or the other gonadotropin. We showed that most of the responsive genes were expressed in somatic cells and exhibited relevant patterns during the seasonal reproductive cycle. Some genes preferentially modulated by Lh could be involved in testicular cell fate (pvrl1 and bty) or sperm maturation (ehmt2 and racgap1) and will deserve further examination. Besides Fsh's effects on the steroidogenic pathway, our study demonstrates that Fsh coordinates relevant stimulatory and inhibitory paracrine factors known to regulate early germ cell proliferation and differentiation. Some of these genes belong to major regulatory pathways including the Igf pathway (igf1b/igf3 and igfbp6), the Tgfb pathway (amh, inha, inhba, and fstl3), the Wnt pathway (wisp1), and pleiotrophin (mdka).

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“…Levels of 11-ketotestosterone (11KT) were measured by specific RIA in culture media according to Fostier et al (1982). Each sample was assayed in duplicates.…”

Section: Steroid Measurementmentioning

confidence: 99%

Fsh and Lh have common and distinct effects on gene expression in rainbow trout testis

Sambroni¹,

Rolland²,

Lareyre³

et al. 2012

Journal of Molecular Endocrinology

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“…Fish were euthanised using ethylene glycol-monophenyl ether (1.3 ml l -1 water) and plasma samples stored at -20°C. Plasma cortisol concentrations were measured by a specific radio-immunoassay adapted from Fostier et al (1982). Blood samples were also taken from six additional fish per tank for other analyses.…”

Section: Long-term Experimentsmentioning

confidence: 99%

Effects of repeated hypoxic shocks on growth and metabolism of turbot juveniles

Ruyet

2003

Aquatic Living Resources

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Turbot juveniles (45 g) were exposed for 41 d (17°C, 34‰ salinity) to constant normoxic (100-100% air saturation, 100-100) or moderate hypoxic (75-75% air saturation, 75-75) conditions and to repeated hypoxic shocks (20% saturation for 1 h, 5 d per week) from normoxic (100-20% air saturation, 100-20) or moderate hypoxic (75-20% air saturation, 75-20) conditions. A normoxic group was feed restricted (100-FR). Mass increase of 100-100 and 75-75 groups fed to satiation was not significantly different. In comparison, it was significantly lower in the 100-20 and 75-20 groups (NS between the two hypoxic shocks groups). Intermediate results were obtained in the 100-100-FR group. The lowest mass increase under hypoxic shocks was explained by a significant decrease in both feed intake and food conversion efficiency (FCE). FCE was lower in the two hypoxic groups, but only the 75-20 group was significantly different from all the other groups. There was no sign of stress and no change in the physiological status of fish in any group. When challenged, pre-conditioning of turbot to regular hypoxic shocks extended survival time, slightly but significantly, for 50% of the population. It was 8 h longer in starved than in fed fish. When reared for 1 year in normoxic water, the growth rate of post-challenged survivors was dependent on pre-conditioning: day 0-375 specific growth rate was significantly higher in the two groups acclimated to repeated hypoxic shocks. In the second experiment, it was shown that exposure to 20% air saturation for 12 h led to major physiological changes within 4 h: a significant decrease in plasma total CO 2 and increase in plasma lactate contributing in maintaining blood pH stable, and a significant increase in osmolarity and chloride concentration. When returned to normoxic water, the recovery capacity of the fish was high: plasma osmolarity and total CO 2 returned to pre-exposure levels within 1 h. The results are discussed in terms of turbot capacity to cope with repeated hypoxic shocks and to acclimate. RésuméEffets de chocs hypoxiques répétés sur la croissance et le métabolisme du turbot juvénile. Des turbots (45 g) ont été élévés pendant 41 jours (17°C, et 34 salinité) en normoxie (100-100 % de la saturation) ou hypoxie modérée (75-75), ou soumis à des chocs hypoxiques de 20 % de la saturation pendant une heure 5 fois par semaine (100-20 et 75-20). Un lot en normoxie reçoit la ration alimentaire du lot 100-20 (100-R). La croissance pondérale n'a pas été perturbée par l'hypoxie modérée à niveau constant mais l'a été par les chocs hypoxiques répétés. La baisse de croissance observée résulte d'un effet cumulé d'une baisse de l'appétit et d'une diminution de l'efficacité alimentaire (lot 75-20 significativement différent des autres lots). Aucune modification de l'état physiologique n'a été mise en évidence (absence de stress). Une mise à l'épreuve en fin d'expérience (20 % de la saturation) a montré qu'un préconditionnement à différentes conditions d'oxygénation a un effet bénéfique faible ma...

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“…This difference is probably related to the hindrance of testicular development in delayed maturing group. T and 11-KT play an important role during spermatogenesis in teleosts (Idler et al 1961,1971, Arai, 1967, Fostier et al 1982, Lou et al 1986). The very low levels of 11-KT in delayed maturing group may also explain the observations of Usui & Goto (1987) and Goto (1987) that large-sized immature males did not show secondary sexual characteristics such as large mouth and long anal fin, in contrast to the early maturing group, especially during the spawning period.…”

Section: Discussionmentioning

confidence: 99%

A comparison of the annual changes in testicular activity and serum androgen levels between the early and delayed maturing groups of male Cottus hangiongensis

1992

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SynopsisAnnual changes in testicular activity and concentration of two serum androgens were monitored in two groups of the river-sculpin Cottus hangiongensis collected from the upper and lower reaches of a river at southern Hokkaido, Japan. One of them (early maturing group) underwent testicular maturation with aberrant spermatids and spermatid masses produced during the reproductive cycle. Moreover, regular seasonal changes in serum testosterone and 11-ketotestosterone concentrations were observed. On the other hand, in the other group (delayed maturing group), although body size of the fish was large enough to undergo reproduction, annual changes in gonadosomatic index and testicular activity did not vary much. During the months of active testicular development in the early maturing group, spermatogenesis was observed to begin in some regions of the testes of delayed maturing fish, but always resulted in the formation of aberrant spermatids and spermatid masses. Moreover, concentration of serum androgens did not significantly vary throughout the year. Results suggest that low androgen production is a proximal factor for delayed sexual maturity in the delayed maturing group, and that the occurrence of aberrant spermatids and spermatid masses during spermatogenesis is not linked to the delayed maturity.

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Plasma 11-oxotestosterone and gonadotropin during the beginning of spermiation in rainbow trout (Salmo gairdneri R.)

Cited by 99 publications

References 24 publications

Fsh and Lh have common and distinct effects on gene expression in rainbow trout testis

Fsh and Lh have common and distinct effects on gene expression in rainbow trout testis

Effects of repeated hypoxic shocks on growth and metabolism of turbot juveniles

A comparison of the annual changes in testicular activity and serum androgen levels between the early and delayed maturing groups of male Cottus hangiongensis

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