Plasma 11-Deoxycorticosteroid and ACTH Response to Metyrapone (Plasma Metyrapone Test)

Simultaneous Determinations of Plasma ACTH and GH Levels after an Oral Metyrapone

“…This may also exclude the possibility that an increase in endogenous ACTH stimulates GH release, though Zahnd et al(1969) (Berson and Yalow, 1968) or longer (Berson and Yalow, 1968;Liddle et al, 1962;Strott et al, 1969;Jubiz et al, 1970a, and b) to stimulate the release of ACTH by a negative feedback mechanism. Therefore, the of metyrapone may not be caused by the negative feedback mechanism.…”

THERAPY OF ENDOCRINE DISEASE: Steroidogenesis enzyme inhibitors in Cushing's syndrome

“…For this reason metyrapone was initially considered to be ineffective for CD. Therefore, it was primarily used as a test for pituitary-adrenal reserve and the differential diagnosis of ACTH-dependent CS (48,49,50). Later studies in 18 patients with CS treated with a combination of metyrapone and aminoglutethimide, an antiepileptic and steroidogenesis enzyme inhibitor, indicated that metyrapone may be effective in improving clinical and biochemical features of CS (51).…”

A Simplified Metyrapone Test with Determination of Plasma 11-Deoxycortisol (Metyrapone Test with Plasma S)

“…With a simple, accurate and highly reproducible method, relative specificity for quantitation of plasma S in post-metyrapone samples of plasma was proved by comparison of results obtained before and after chromatographic isolation of S. This high degree of specificity was accomplished by combining a CPB radioassay with a simple solvent extraction procedure which removes from the final extract large quantities of corticosteroids both more and less polar than S. Other extraction procedures that have been reported have the disadvantage that the recovery of S is lower, and other steroids that are found in plasma and would be assayed are extracted (1,6).…”